Studies of the mechanism for the establishment of sexual differences in hippocampal learning processes

• Project/Area Number: 17K08577
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental physiology(including physical medicine and nutritional physiology)
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: Funabashi Toshiya 聖マリアンナ医科大学, 医学部, 教授 (70229102)
• Co-Investigator(Kenkyū-buntansha): 長谷 都 聖マリアンナ医科大学, 医学部, 准教授 (20450611) ; 萩原 裕子 聖マリアンナ医科大学, 医学部, 助教 (90468207)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 海馬 / エストロジェン / シナプス / 空間学習 / LTP / 性分化 / 性差 / 餌の形状 / テストステロン / オープンフィールド / RI / 受動的回避学習 / 雄性化 / 雌性 / パッチクランプ / スライス / AMPA受容体 / 学習 / 養育環境 / エストロゲン / 環境 / 可塑性

Outline of Final Research Achievements

In rodents, sex steroid hormones control many functions regulated by the central nervous system. The sex difference in the brain functions is at least in part as the result of steroid hormone milieu during prenatal development and immediately after birth. We examined in the present study whether this sex steroid hormone milieu affected the sex specific alteration of hippocampal neuronal function by feeding experiences. As the results, we revealed that the sex specific alteration by the feeding experience in spatial learning regulated by the hippocampus was at least in part as the result of the sex difference in steroid hormone milieu immediately after birth.

Academic Significance and Societal Importance of the Research Achievements

実験動物のラットなどの齧歯類は，脳によって調節される機能の少なくとも一部は性腺ステロイドホルモンに強く影響され，それは，出生時期の性腺ステロイドホルモン環境の違いによる脳の性分化の結果と考えられる。本研究から，性特異的な飼育環境による海馬機能の変容が，一部は出生時期の性腺ステロイドホルモン環境の違いによる性分化の結果である事がわかった意義がある。

Research Products

• [Journal Article] Age-related decrease in muscle satellite cells is accompanied with diminished expression of early growth response 3 in mice (2020)
  • Author(s): Ogura Y., Sato S., Kurosaka M., Kotani T., Fujiya H., Funabashi T.
  • Journal Title: Mol. Biol. Rep. Volume: 47 Issue: 2 Pages: 977-986
  • DOI: 10.1007/s11033-019-05189-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reduced Luteinizing Hormone Induction Following Estrogen and Progesterone Priming in Female-to-Male Transsexuals (2018)
  • Author(s): Funabashi Toshiya、Sakakibara Hideya、Hirahara Fumiki、Kimura Fukuko
  • Journal Title: Frontiers in Endocrinology Volume: 9
  • DOI: 10.3389/fendo.2018.00212
  • Peer Reviewed / Open Access
• [Journal Article] Short-term fasting decreases excitatory synaptic inputs to ventromedial tuberoinfundibular dopaminergic neurons and attenuates their activity in male mice. (2018)
  • Author(s): Kubota T, Fukushima A, Hagiwara H, Kamiya Y, Furuta M, Miyazaki T, Fujioka H, Fujiwara SE, Funabashi T, Akema T.
  • Journal Title: Neurosci Lett. Volume: 10 (671) Pages: 70-75
  • DOI: 10.1016/j.neulet.2018.02.017
  • Peer Reviewed / Open Access
• [Journal Article] Reduced Luteinizing Hormone Induction Following Estrogen and Progesterone Priming in Female-to-Male Transsexuals (2018)
  • Author(s): Toshiya Funabashi, Naoya Sakakibara, Fumiki Hirahara and Fukuko Kimura
  • Journal Title: Frontiers in Endocrinology Volume: in press
  • Peer Reviewed / Open Access
• [Journal Article] Prostaglandin E2 modulates presynaptic regulation of GnRH neurons via EP4 receptors in accordance with estrogen milieu (2017)
  • Author(s): Fujioka H, Funabashi T, Akema T
  • Journal Title: Neuroscience Volume: 360 Pages: 139-145
  • DOI: 10.1016/j.neuroscience.2017.07.025
  • Peer Reviewed
• [Presentation] Effects of Omega-3 fatty acids feeding on high-fat induced impairment of glucose tolerance and behavioral performances (2020)
  • Author(s): Atsushi Fukushima, Miyako Furuta, Hitomi Fujioka, Toshiya Funabashi
  • Organizer: 第97回日本生理学会大会
• [Presentation] STAT6は筋芽細胞の分化・融合を負に制御する (2020)
  • Author(s): 黒坂光寿, 小倉裕司, 幸田和久, 舩橋利也
  • Organizer: 第97回日本生理学会大会
• [Presentation] Effects of the experience of pregnancy-delivery-nurturing on excitatory synaptic inputs to tuberoinfundibular dopaminergic neurons (2019)
  • Author(s): M Furuta, C Kakehashi and T Funabashi
  • Organizer: The 49th Society for Neuroscience
  • Int'l Joint Research
• [Presentation] Atypical Inhibitor of kappa beta is associated with denervation-induced muscle atrophy in mice (2019)
  • Author(s): Ogura Y., Kakehashi C., Funabashi T.
  • Organizer: 48th European muscle conference
  • Int'l Joint Research
• [Presentation] Role of CRH neurons in the dorsolateral BST on the sex difference in pain response in mice. (2019)
  • Author(s): Hiroko Hagiwara, Toshiya Funabashi
  • Organizer: 第42回日本神経科学大会
• [Presentation] ntracerebroventricular infusion of PDGF-AA induces weight gain by reducing locomotor activity and decreases MEF2C expression in the dorsomedial hypothalamus. (2019)
  • Author(s): Atsushi Fukushima, Toshiya Funabashi
  • Organizer: 第42回日本神経科学大会
• [Presentation] Involvement of kiss-1 neurons in the suppression of LH surge by LPS and amelioration of this suppression by minocycline pretreatment in ovarian steroid-primed ovariectomized rats (2019)
  • Author(s): Hitomi Fujioka, Atsushi,Fukushima, Toshiya Funabashi, Tatsuo Akema
  • Organizer: 第42回日本神経科学大会
• [Presentation] Ketogenic diet induces slow-type shift of skeletal muscle in male rat (2019)
  • Author(s): Yuji Ogura, Mitsutoshi Kurosaka, Chiaki Kakehashi, Ryo Kakigi, Tatsuo Akema, Toshiya Funabashi
  • Organizer: FAOPS2019
• [Presentation] STAT6 promotes myoblast fusion via regulating myoblast differentiation (2019)
  • Author(s): Mitsutoshi Kurosaka, Yuji Ogura, Kazuhisa Koda, Toshiya Funabashi
  • Organizer: FAOPS2019
• [Presentation] Involvement of early growth response 3 in myoblast proliferation (2018)
  • Author(s): Yuji Ogura, Mitsutoshi kurosaka, Tatsuo Akema, Toshiya Funabashi
  • Organizer: 第41回日本分子生物学会年会
• [Presentation] The effect of maternal experiences on spatial learning and hippocampal neural plasticity. (2018)
  • Author(s): M Furuta, A Fukushima, T Akema, T Funabashi
  • Organizer: The 48th Society for Neuroscience
• [Presentation] LPS急性ストレスによるGnRHサージ発生機構の活動抑制とミノサイクリン前投与による抑制解除へのkiss1ニューロンの関与 (2018)
  • Author(s): 藤岡仁美、舩橋利也、明間立雄
  • Organizer: 第45回日本神経内分泌学会学術集会
• [Presentation] Possible involvement of early growth response 3 in age-related reduction of muscle satellite cells (2018)
  • Author(s): Ogura Y, Kurosaka M, Shuichi S, Kotani T, Fujiya H, Funabashi T
  • Organizer: 2018 New Directions in Biology and Disease of Skeletal Muscle Conference
• [Presentation] 血小板由来成長因子受容体α (PDGFRα) を介した細胞内シグナルは行動量を変化させることによりマウス視床下部による摂食の調節に関与する (2018)
  • Author(s): 福島篤, 明間立雄, 舩橋利也
  • Organizer: 第45回日本神経内分泌学会
• [Presentation] 血小板由来成長因子α受容体を介した細胞内シグナルは雄マウスの視床下部による摂食の調節に関与する (2018)
  • Author(s): 福島篤, 萩原裕子, 明間立雄, 舩橋利也
  • Organizer: 第41回日本神経科学大会
• [Presentation] Expression of platelet-derived growth factor receptor α (PDGFRα)-immunoreactivity (ir) is suppressed by refeeding after 48-h fasting in the hypothalamus of male mice (2018)
  • Author(s): Atsushi Fukushima, Hiroko Hagiwara & Toshiya Funabashi
  • Organizer: 第95回生理学会大会
• [Presentation] 骨格筋再生過程におけるTRPV1の役割 (2017)
  • Author(s): 小倉 裕司, 黒坂 光寿, 明間 立雄, 舩橋 利也
  • Organizer: 生命科学系学会合同年次大会（ConBio2017）
• [Presentation] ACL切断ラットにおける膝関節周囲筋群の長期経過観察―ACL切断肢, 対側肢および健常肢の比較― (2017)
  • Author(s): 大野真弘, 藤谷博人, 後藤勝正, 黒坂光寿, 小倉裕司, 谷田部かなか, 工藤貴章, 小林創, 内野彩, 油井直子, 船橋利也, 幸田和久, 明間立雄, 仁木久照, 武者春樹
  • Organizer: 第32回日本整形外科学会基礎学術集会
• [Presentation] 微弱電流刺激と高気圧酸素療法の併用による骨格筋損傷の修復促進効果 (2017)
  • Author(s): 工藤貴章, 藤谷博人, 後藤勝正, 黒坂光寿, 小倉裕司, 谷田部かなか, 大野真弘, 小林創, 船橋利也, 幸田和久, 仁木久照, 武者春樹
  • Organizer: 第32回日本整形外科学会基礎学術集会
• [Presentation] Egr3はNF-kB経路を介して筋芽細胞の増殖を制御する (2017)
  • Author(s): 小倉裕司, 黒坂光寿, 明間立雄, 舩橋利也
  • Organizer: 第72回日本体力医学会大会
• [Presentation] CRF-Venus ΔNeoマウスの分界条床核外側部CRHニューロンのシナプス入力の性差ーホルマリンテストを用いて (2017)
  • Author(s): 萩原裕子, 崎村建司, 井樋慶一, 舩橋利也
  • Organizer: 第40回日本神経科学大会
• [Book] 標準生理学 第9版 (2019)
  • Author(s): 本間 研一
  • Total Pages: 1172
  • Publisher: 医学書院
  • ISBN: 9784260034296
• [Book] ガイトン生理学 (2018)
  • Author(s): John E Hall
  • Total Pages: 1060
  • Publisher: エルゼビア・ジャパン
  • ISBN: 9784860347741