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Physiological mechanism and social function of vasopressin and oxytocin neuron

Research Project

Project/Area Number 17K08582
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental physiology(including physical medicine and nutritional physiology)
Research InstitutionUniversity of Occupational and Environmental Health, Japan

Principal Investigator

Maruyama Takashi  産業医科大学, 医学部, 准教授 (20533194)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsバゾプレッシン / オキシトシン / 薬理遺伝学 / 視床下部 / 社会行動 / 脳組織透明化 / 環境生理 / 行動生理
Outline of Final Research Achievements

In order to elucidate the physiological role of vasopressin (AVP) neuron and oxytocin (OXT) neuron on social behavior and stress responses, we conducted experiments to observe changes in expression of AVP and OXT neurons in hypothalamus. Regarding AVP, the expression after various stresses was observed, in particular, changes in AVP expression and various feeding-related neuropeptides during renal dysfunction were revealed. In addition, with the aim of establishing an experimental model using chemogenetic techniques (DREADDs), we have established a novel transgenic rat line which expresses hM3Dq of which ligand is clozapine-N-oxide (CNO) in AVP neurons. This AVP-hM3Dq-mCherry transgenic rat was demonstrated to be activated the AVP neuron after CNO injection. Now we are also developing the OXT-hM3Dq-mCherry transgenic rat. These transgenic rats must be useful experimental animal model to elucidate the physiological role of AVP and OXT neuron on social behavior.

Academic Significance and Societal Importance of the Research Achievements

視床下部で合成される神経ペプチドであるバゾプレッシン及びオキシトシンの生理的役割を解明するため、様々なストレス負荷を与えた際の神経細胞の反応を確認し、腎機能障害の際の反応など新たな知見が得られた。また、薬理遺伝学的にバゾプレッシン及びオキシトシンニューロンを活性化する遺伝子改変ラットが開発されることにより、バゾプレッシン及びオキシトシンの社会行動における役割解明を行う際の有用な動物実験モデルになると考えられる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (5 results)

All 2019 2017

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 3 results)

  • [Journal Article] Changes in gene expressions of hypothalamic neuropeptides controlling feeding behaviors in bilateral nephrectomized rats2019

    • Author(s)
      Ueno Hiromichi、Miyamoto Tetsu、Sanada Kenya、Nakazono Kazutoshi、Tanaka Kentaro、Nishimura Haruki、Nishimura Kazuaki、Sonoda Satomi、Yoshimura Mitsuhiro、Maruyama Takashi、Serino Ryota、Otsuji Yutaka、Ueta Yoichi
    • Journal Title

      Neuroscience Letters

      Volume: 711 Pages: 134426-134426

    • DOI

      10.1016/j.neulet.2019.134426

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The fluorescent visualization of oxytocin neurons and pain control study in rat model2019

    • Author(s)
      Takashi Maruyama、Yoichi Ueta
    • Journal Title

      Neurology

      Volume: 90(3) Pages: 239-245

    • NAID

      40022273385

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Effects of acute kidney dysfunction on hypothalamic arginine vasopressin synthesis in transgenic rats2019

    • Author(s)
      Ueno Hiromichi、Serino Ryota、Sanada Kenya、Akiyama Yasuki、Tanaka Kentaro、Nishimura Haruki、Nishimura Kazuaki、Sonoda Satomi、Motojima Yasuhito、Saito Reiko、Yoshimura Mitsuhiro、Maruyama Takashi、Miyamoto Tetsu、Tamura Masahito、Otsuji Yutaka、Ueta Yoichi
    • Journal Title

      The Journal of Physiological Sciences

      Volume: 69 Issue: 3 Pages: 531-541

    • DOI

      10.1007/s12576-019-00675-8

    • NAID

      40021874305

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of oestrogen-dependent vasopressin on HPA axis in the median eminence of female rats2019

    • Author(s)
      Nishimura Kazuaki、Yoshino Kiyoshi、Sanada Kenya、Beppu Hiroki、Akiyama Yasuki、Nishimura Haruki、Tanaka Kentaro、Sonoda Satomi、Ueno Hiromichi、Yoshimura Mitsuhiro、Maruyama Takashi、Ozawa Hitoshi、Ueta Yoichi
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1

    • DOI

      10.1038/s41598-019-41714-z

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Activation of endogenous arginine vasopressin neurons inhibit food intake: by using a novel transgenic rat line with DREADDs system2017

    • Author(s)
      Yoshimura Mitsuhiro、Nishimura Kazuaki、Nishimura Haruki、Sonoda Satomi、Ueno Hiromichi、Motojima Yasuhito、Saito Reiko、Maruyama Takashi、Nonaka Yuki、Ueta Yoichi
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 15728-15728

    • DOI

      10.1038/s41598-017-16049-2

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access

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Published: 2017-04-28   Modified: 2021-02-19  

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