| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In this project, I developed electrophysiological indices for human iPS cell-derived cardiomyocytes’ monolayer sheets to outline the pro- and/or anti-arrhythmic properties of drugs. Each cell sheet was prepared on each microelectrode-array probe, and then paced with programmed stimuli via a pair of adjacent electrodes before and after drug treatment. I examined the electrophysiological indices of the cell sheet for detecting Na+ channel blockade with its characteristics, blockade of K+ and Ca2+ channels, and their balance in the repolarization phase using clinical antiarrhythmics. I then assessed an anticancer agent and a Kampo preparation with the indices and detected their multichannel blocking actions. The obtained results indicated that the cell sheets with the indices could be valuable to predict pro- and/or anti-arrhythmic action of a substance in the human intact heart.
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