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Analyisis of regulome through E/Id protein axis in Treg cells

Research Project

Project/Area Number 17K08629
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General medical chemistry
Research InstitutionKyoto University

Principal Investigator

Kazuko Miyazaki  京都大学, ウイルス・再生医科学研究所, 研究員 (00311811)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords転写制御 / 遺伝子発現制御 / Treg / 制御性T細胞 / E2A, Id2, Id3
Outline of Final Research Achievements

The analysis of gene expression profiles and open chromatin regions in Id2/Id3-deficient Treg cells demonstrated that a set of effector molecules in effector Treg (eTreg) cells could be regulated by E-Id3 protein axis. Then, we examined the chromatin interactions in CXCR5 gene locus, which was one of the activation-related genes in eTreg cells and could be the direct target of E2A. From the result of Hi-C analysis that CXCR5 gene resided in the permissive “A” compartment in resting cells, we conclude that Id-proteins possibly function as a gatekeeper of eTreg cell differentiation.
In addition, we proved that Id2 and Id3 expression in Treg cells plays an important role in the suppression of systemic inflammation not only in childhood but also in adulthood, which was revealed by the analysis of Id2-/Id3-flox Foxp3Cre/DTR mouse line.

Academic Significance and Societal Importance of the Research Achievements

Treg細胞におけるエンハンサー機能調節の制御機構をゲノムワイドに解析した結果は、アレルギー反応の抑制における転写制御のメカニズムの解明につながり、社会的意義が深いと思われる。また、作成された新規マウスモデルで、Id2/Id3による Treg細胞の機能制御が全身性の炎症抑制に必須であることを証明したことは、学術的に意義深い。それを用いて、成獣期において実際にどのようにTh2炎症が起こるのかを詳細に解析することが可能になり、今後の研究の発展が期待できる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (6 results)

All 2019 2017 Other

All Int'l Joint Research (2 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Book (1 results) Remarks (2 results)

  • [Int'l Joint Research] UT Southwestern Medical Center(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of California, San Diego/Baylor Research Institute(米国)

    • Related Report
      2017 Research-status Report
  • [Journal Article] The E-Id Protein Axis Specifies Adaptive Lymphoid Cell Identity and Suppresses Thymic Innate Lymphoid Cell Development2017

    • Author(s)
      Miyazaki Masaki、Miyazaki Kazuko、Chen Kenian、Jin Yi、Turner Jacob、Moore Amanda J.、Saito Rintaro、Yoshida Kenichi、Ogawa Seishi、Rodewald Hans-Reimer、Lin Yin C.、Kawamoto Hiroshi、Murre Cornelis
    • Journal Title

      Immunity

      Volume: 46 Issue: 5 Pages: 818-834.e4

    • DOI

      10.1016/j.immuni.2017.04.022

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Book] 医学のあゆみ 制御性T細胞ー研究の現在ー2019

    • Author(s)
      坂口志文 堀昌平 編
    • Total Pages
      264
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2018 Research-status Report
  • [Remarks] 京都大学ウイルス・再生医科学研究所 研究成果

    • URL

      https://www.infront.kyoto-u.ac.jp/achievements/post-1717/

    • Related Report
      2017 Research-status Report
  • [Remarks] ライフサイエンス新着論文レビュー

    • URL

      http://first.lifesciencedb.jp/archives/16546

    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2022-02-21  

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