Regulation of male germ cell differentiation by selective mRNA export

• Project/Area Number: 17K08630
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Osaka Prefecture University (2018-2019); Osaka University (2017)
• Principal Investigator: Katahira Jun 大阪府立大学, 生命環境科学研究科, 准教授 (30263312)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 核―細胞質間輸送 / 遺伝子発現制御 / 核－細胞質間輸送 / mRNA / 発現制御

Outline of Final Research Achievements

The aim of present study was elucidation of the role of “selective mRNA export” for differentiation and development of male germ cells. To this end, the usefulness of a newly developed method for identification of target mRNA was evaluated. ES cell lines harboring mRNA exporter-base modifying enzyme fusion TGs were established. By using these ES cell lines several TG mouse lines were also established, however, it turned out that the TGs were only poorly expressed in testicular tissues, indicating further optimization of the expression system is required to fully exploit the system for future analysis.

Academic Significance and Societal Importance of the Research Achievements

改良の余地が依然として多く残されているものの、本研究で採用したRNA標識法が、動物個体を用いたRNA輸送の研究を行なう上で、有望なツールであることを検証することができた。また、研究過程で作成したノックアウトマウスを精子形成過程や幹細胞維持機構の研究等に利用可能な研究材料として公的機関に寄託し、広く利用できるようにすることで、研究コミュニティーに貢献することができた。

Research Products

• [Journal Article] Human THO maintains the stability of repetitive DNA (2020)
  • Author(s): Katahira J, Senokuchi K, Hieda H.
  • Journal Title: Genes to Cells Volume: 25 Issue: 5 Pages: 334-342
  • DOI: 10.1111/gtc.12760
  • Peer Reviewed / Open Access
• [Journal Article] Human THO coordinates transcription termination and subsequent transcript release from the HSP70 locus (2019)
  • Author(s): Katahira, J., Ishikawa, H., Tsujimura, K., Kurono, S., Hieda, M.
  • Journal Title: Genes Cells Volume: 24 Issue: 4 Pages: 272-283
  • DOI: 10.1111/gtc.12672
  • Peer Reviewed / Open Access
• [Journal Article] Relationship between PIWIL4-mediated H3K4me2 demethylation and piRNA-dependent DNA methylation. (2018)
  • Author(s): Nagamori, I., Kobayashi, H., Nishimura, T., Yamagishi, R., Katahira, J., Kuramochi-Miyagawa, S., Kono, T., Nakano, T.
  • Journal Title: Cell Reports Volume: 25 Issue: 2 Pages: 350-356
  • DOI: 10.1016/j.celrep.2018.09.017
  • Peer Reviewed / Open Access
• [Presentation] Human THO coordinates transcription termination and subsequent transcript release from the HSP70 locus (2018)
  • Author(s): Katahira, J., Ishikawa, H., Tsujimura, K.
  • Organizer: 日本分子生物学会
• [Presentation] SUN1バリアントがLINC 複合体の機能的多様性に担う役割の解析 (2017)
  • Author(s): 西岡優、今泉大将、今田順子、片平じゅん、松浦成昭、檜枝美紀
  • Organizer: 染色体ワークショップ