Towards an improvement in the low sensitivity to chemotherapy in ovarian clear cell carcinoma

• Project/Area Number: 17K08709
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Teikyo University
• Principal Investigator: Koike Ryoko 帝京大学, 医学部, 助手 (80535063)
• Co-Investigator(Kenkyū-buntansha): 坂本 優 公益財団法人佐々木研究所, 附属研究所, 副院長(移行) (20260101) ; 綾部 琢哉 帝京大学, 医学部, 教授 (00272568) ; 中川 俊介 帝京大学, 医学部, 講師 (70270874)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 卵巣明細胞腺癌 / 蛍光標識2次元ディファレンシャルゲル電気泳動法 / パクリタキセル / NNMT / 卵巣明細胞癌 / 抗がん剤耐性 / 化学療法

Outline of Final Research Achievements

Fluorescence-labeled two-dimensional differential gel electrophoresis showed that the expression of lab-named protein 1 was higher in ovarian clear cell carcinoma cell lines 1 and 2, which are poor responders to paclitaxel, than in cell lines 3 and 4, which are good responders to paclitaxel. The expression of lab-name protein 1 was higher in cell line 1 and 2 than in cell line 3 and 4. Kaplan-Meier survival analysis of 39 cases of ovarian adenocarcinoma showed that there were no deaths in the group with low expression of lab name protein1.

Academic Significance and Societal Importance of the Research Achievements

卵巣明細胞腺癌ではラボネームprotein1が下がるとパクリタキセルの感受性が上がり、生命予後がよくなる可能性がある。

Research Products

• [Journal Article] Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-like Cells using the Three-dimensional Co-culture System, NICO-1 (2020)
  • Author(s): Miyagawa Yuko、Nagasaka Kazunori、Yamawaki Kaoru、Mori Yutaro、Ishiguro Tatsuya、Hashimoto Kei、Koike Ryoko、Fukui Siho、Sugihara Takeru、Ichinose Takayuki、Hiraike Haruko、Kido Koichiro、Okamoto Koji、Enomoto Takayuki、Ayabe Takuya
  • Journal Title: Journal of Visualized Experiments Volume: 166 Issue: 166 Pages: 166-166
  • DOI: 10.3791/61751
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Array comparative genomic hybridization analysis discloses chromosome copy number alterations as indicators of patient outcome in lymph node-negative breast cancer (2019)
  • Author(s): Ryoko Kikuchi-Koike, Kazunori Nagasaka, Hitoshi Tsuda, Yasuyuki Ishii, Masaru Sakamoto, Yoshihiro Kikuchi, Shiho Fukui, Yuko Miyagawa, Haruko Hiraike, Takayuki Kobayashi, Takayuki Kinoshita, Yae Kanai, Tatsuhiro Shibata, Issei Imoto, Johji Inazawa, Osamu Matsubara and Takuya Ayabe
  • Journal Title: BMC Cancer Volume: 19 Issue: 1 Pages: 521-521
  • DOI: 10.1186/s12885-019-5737-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The proteasome deubiquitinase inhibitor bAP15 downregulates TGF-β/Smad signaling and induces apoptosis via UCHL5 inhibition in ovarian cancer. (2019)
  • Author(s): Fukui S, Nagasaka K, Miyagawa Y, Kikuchi-Koike R, Kawata Y, Kanda R, Ichinose T, Sugihara T, Hiraike H, Wada-Hiraike O, Sasajima Y, Ayabe T
  • Journal Title: Oncotarget Volume: 10 Issue: 57 Pages: 5932-5948
  • DOI: 10.18632/oncotarget.27219
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Array comparative genomic hybridization analysis discloses chromosome copy number alterations as indicators of patient outcome in lymph node-negative breast cancer (2019)
  • Author(s): Ryoko Kikuchi-Koike1,2,3, Kazunori Nagasaka1§, Hitoshi Tsuda2, Yasuyuki Ishii4, Masaru Sakamoto3,5,6, Yoshihiro Kikuchi7, Shiho Fukui1, Yuko Miyagawa1, Haruko Hiraike1, Takayuki Kobayashi2, Takayuki Kinoshita8, Yae Kanai9, Tatsuhiro Shibata10, Issei Imoto11, Johji Inazawa12, Osamu Matsubara2, Takuya Ayabe1
  • Journal Title: BMC cancer Volume: 9 Pages: 9-9
  • Peer Reviewed / Open Access