| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
The most serious issue in domino liver transplantation using graft liver from patients with familial amyloid polyneuropathy (FAP) is transmission of transthyretin-related amyloidosis to recipients. In this study, to elucidate pathophysiology of early-stage amyloid deposits in domino related FAP, we carried out pathological and biochemical evaluations on gastroduodenal amyloid of 8 domino recipients. Deposited amyloid was extracted using laser micro-dissection system and analyzed by tandem mass spectrometry (LC-MS/MS). In most patients, variant TTR was main component of early-stage amyloid proteins. By detailed pathological re-examination, it was revealed that early stage amyloid deposition may often be overlooked on routine histopathological examinations.
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