| Project/Area Number |
17K08742
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Hiroshima University |
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Principal Investigator |
Takeshima Yukio 広島大学, 医系科学研究科(医), 教授 (70236462)
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| Co-Investigator(Kenkyū-buntansha) |
AMATYA VISHWA・J 広島大学, 医系科学研究科(医), 講師 (90403625)
櫛谷 桂 広島大学, 医系科学研究科(医), 助教 (00508179)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | mesothelioma / adenocarcinoma / antibody / differential diagnosis / immunohistochemistry / 中皮腫 / 遺伝子発現解析 / マイクロアレー / 免疫組織化学的染色 / 網羅的遺伝子発現解析 / 病理診断 / 免疫組織化学的診断 / 新規マーカー |
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| Outline of Final Research Achievements |
By using whole genone expression anaysis, we found gene showing differentially between epithelioid mesothelioma and lung adenocarcinoma, and staind mesothelioma and adenocarcinoma tissues, and clarified sensitivity and specificity. As the result, in mesothelioma, DAB2, Intelectin-1, SOX6, PRG4, Annexin-8 gene and protein expression in mesothelioma, and MUC4, MUC21 in adenocarcinoma were significantly higher. These novel markers have higher sensititity and specificity than convential differential markers. Therefore, these novel markes could be used for differential diagnosis between mesothelioma and lung adenocarcinoma.
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| Academic Significance and Societal Importance of the Research Achievements |
中皮腫の病理診断は各種抗体を用いて行うが、現在用いられている抗体のみでは鑑別診断が困難な場合がある。そこで、網羅的遺伝子発現解析を基本にして、鑑別診断に有用なマーカーを見いだした。その結果、中皮腫ではDAB2, Intelectin-1, SOX6, PRG4, Annexin-8が、 肺腺癌ではMUC4, MUC21が発現が有意に高いことが明らかとなり、これら新規マーカーが、中皮腫と肺腺癌の鑑別診断に利用可能であることが示された。
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