An attempt at personalized treatment of salivary gland cancer using Liquid based cytology

• Project/Area Number: 17K08753
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Japanese Foundation for Cancer Research
• Principal Investigator: SATO Yukiko 公益財団法人がん研究会, 有明病院 病理部, 副医長 (30365712)
• Project Period (FY): 2017-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 唾液腺腫瘍 / LBC / 細胞診 / FISH / 悪性度分類 / 唾液腺 / 融合遺伝子 / 唾液腺癌

Outline of Final Research Achievements

The purpose of this study is to improve the diagnostic accuracy of cytology in salivary gland tumors and to help in treatment selection and prognosis prediction.The test performance of salivary gland fine needle aspiration (FNA) shows a range of sensitivities and specificities depending on a wide variety of factors, including the experience level of the physician performing the FNA and the pathologist.The 2021 ASCO guidelines state that pathologists may perform ancillary testing (immunohistochemistry or molecular studies) on FNA and core needle biopsies　(CNBs) to support diagnoses and risk of malignancies (ROMs).
The application of the FISH method in liquefied based cytology specimens that we are setting up can be used for ancillary testing in accordance with the guidelines.We are now at the stage of examining how to apply this technology to actual clinical practice, and plan to accumulate further examples and report on them.

Academic Significance and Societal Importance of the Research Achievements

研究を始めた当初は唾液腺癌という枠で画一された治療がなされていた。最近になって病理学的な組織型や標的となる分子変化による治療の変更が行われるようになった。術前の穿刺吸引細胞診の判定基準はミラノシステムという悪性のリスクを加味した判定様式に変更になった。時代とともに細胞診検体に対する免疫組織化学やFISH法の必要性が増してきている。我々は画一化した方法により形態情報に併せた細胞診の診断精度を向上させることが可能になると考えられる。

Research Products

• [Journal Article] Survival benefit of HER2-targeted or androgen deprivation therapy in salivary duct carcinoma (2022)
  • Author(s): Kawakita Daisuke、Nagao Toshitaka、Takahashi Hideaki、Kano Satoshi、Honma Yoshitaka、Hirai Hideaki、Saigusa Natsuki、Akazawa Kohei、Tani Kaori、Ojiri Hiroya、Tsukahara Kiyoaki、Ozawa Hiroyuki、Okami Kenji、Kondo Takahito、Togashi Takafumi、Fushimi Chihiro、Shimura Tomotaka、Matsuki Takashi、Tada Yuichiro
  • Journal Title: Therapeutic Advances in Medical Oncology Volume: 14 Pages: 175883592211195-175883592211195
  • DOI: 10.1177/17588359221119538
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of novel prognostic and predictive biomarkers in salivary duct carcinoma via comprehensive molecular profiling (2022)
  • Author(s): Kohsaka S, Tada Y, Okami K, et al.
  • Journal Title: NPJ Precis Oncol. Volume: 6 Issue: 1 Pages: 82-82
  • DOI: 10.1038/s41698-022-00324-1
  • Peer Reviewed / Open Access
• [Journal Article] The Role of the EZH2 and H3K27me3 Expression as a Predictor of Clinical Outcomes in Salivary Duct Carcinoma Patients: A Large-Series Study With Emphasis on the Relevance to the Combined Androgen Blockade and HER2-Targeted Therapy (2022)
  • Author(s): Saigusa N, Hirai H, Kohsaka S, et al.
  • Journal Title: Front Oncol Volume: 11 Pages: 779882-779882
  • DOI: 10.3389/fonc.2021.779882
  • Peer Reviewed / Open Access
• [Journal Article] Clinicopathological and genomic features in patients with head and neck neuroendocrine carcinoma. (2021)
  • Author(s): Ohmoto A, Sato Y, Asaka R, Fukuda N, Wang X, Urasaki T, Hayashi N, Sato Y, Nakano K, Yunokawa M, Ono M, Tomomatsu J, Toshiyasu T, Mitani H, Takeuchi K, Mori S, Takahashi S.
  • Journal Title: Modern pathology Volume: 34(1) Pages: 1979-1989
  • Peer Reviewed / Open Access
• [Journal Article] A Case of Buccal Clear Cell Carcinoma Caused by Rare Fusion Gene: EWSR1-CREM. (2021)
  • Author(s): Hoshino M, Inoue K, Kaneda T, Nishimura M, Kusama K, Sakashita H, Sato Y, Takeuchi K, Nagao T, Kikuchi K.
  • Journal Title: Case reports in dentistry Volume: 2021 Pages: 5557247-5557247
  • Peer Reviewed / Open Access
• [Journal Article] Clinical Impact of Cachexia in Head and Neck Cancer Patients Who Received Chemoradiotherapy. (2021)
  • Author(s): Naomi Hayashi, Yasuyoshi Sato, Yu Fujiwara, Naoki Fukuda, Xiaofei Wang, Kenji Nakano, Testuya Urasaki, Akihiro Ohmoto, Makiko Ono, Junichi Tomomatsu, Yukiko Sato, Hiroki Mitani, Takashi Toshiyasu, Shunji Takahashi
  • Journal Title: Cancer management and research Volume: 13 Pages: 8377-8385
  • Peer Reviewed / Open Access
• [Journal Article] The Role of the EZH2 and H3K27me3 Expression as a Predictor of Clinical Outcomes in Salivary Duct Carcinoma Patients: A Large-Series Study With Emphasis on the Relevance to the Combined Androgen Blockade and HER2-Targeted Therapy. (2021)
  • Author(s): Saigusa N, Hirai H, Tada Y, Kawakita D, Nakaguro M, Tsukahara K, Kano S, Ozawa H, Kondo T, Okami K, Togashi T, Sato Y, Urano M, Kajiwara M, Shimura T, Fushimi C, Saito Y, Nagao T.
  • Journal Title: Frontiers in oncology Volume: 11 Pages: 779882-779882
  • Peer Reviewed / Open Access
• [Journal Article] <p>Efficacy of Nivolumab for Head and Neck Cancer Patients with Primary Sites and Histological Subtypes Excluded from the CheckMate-141 Trial</p> (2020)
  • Author(s): Sato Yasuyoshi、Fukuda Naoki、Wang Xiaofei、Urasaki Tetsuya、Ohmoto Akihiro、Nakano Kenji、Yunokawa Mayu、Ono Makiko、Sato Yukiko、Mitani Hiroki、Tomomatsu Junichi、Takahashi Shunji
  • Journal Title: Cancer Management and Research Volume: 12 Pages: 4161-4168
  • DOI: 10.2147/cmar.s249393
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Secretory carcinoma around Stensen’s duct misdiagnosed as salivary duct cyst (2020)
  • Author(s): Shibata E, Morita KI, Kayamori K, Maruiwa M, Michi Y, Sato Y, Takeuchi K, Ikeda T, Harada H, Yoda T.
  • Journal Title: Int J Clin Exp Pathol. Volume: 13 Pages: 2211-2217
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The clinicopathological significance of the adipophilin and fatty acid synthase expression in salivary duct carcinoma. (2020)
  • Author(s): Hirai H、Tada Y、Nakaguro M、Kawakita D、Sato Y、Shimura T、Tsukahara K、Kano S、Ozawa H、Okami K、Sato Y、Fushimi C、Shimizu A、Okamoto I、Takase S、Okada T、Sato H、Imanishi Y、Otsuka K、Watanabe Y、Sakai A、Ebisumoto K、Togashi T、Ueki Y、Ota H、Saigusa N、Takahashi H、Ando M、Urano M、Hanazawa T、Nagao T
  • Journal Title: Virchows Arch Volume: X Issue: 2 Pages: 291-299
  • DOI: 10.1007/s00428-020-02777-w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] TERT Promoter Mutation and Extent of Thyroidectomy in Patients with 1?4 cm Intrathyroidal Papillary Carcinoma (2020)
  • Author(s): Ebina Aya、Togashi Yuki、Baba Satoko、Sato Yukiko、Sakata Seiji、Ishikawa Masashi、Mitani Hiroki、Takeuchi Kengo、Sugitani Iwao
  • Journal Title: Cancers Volume: 12 Issue: 8 Pages: 2115-2115
  • DOI: 10.3390/cancers12082115
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prognostic Implication of Histopathologic Indicators in Salivary Duct Carcinoma (2020)
  • Author(s): Nakaguro Masato、Sato Yukiko、Tada Yuichiro、Kawakita Daisuke、Nagao Toshitaka et al.
  • Journal Title: The American Journal of Surgical Pathology Volume: 44 Issue: 4 Pages: 526-535
  • DOI: 10.1097/pas.0000000000001413
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 【知っておきたい特殊な食道腫瘍・腫瘍様病変】唾液腺型食道腫瘍の臨床病理学的特徴 (2019)
  • Author(s): 河内 洋, 佐藤 由紀子, 中野 薫
  • Journal Title: 胃と腸 Volume: 54 Pages: 1419-1426
  • Peer Reviewed
• [Journal Article] Metastasizing pleomorphic adenoma in the multiple organs A case report on FDG-PET/CT imaging (2018)
  • Author(s): Masamichi Koyama、Takashi Terauchi、Mitsuru Koizumi、 Hiroko Tanaka、Yukiko Sato
  • Journal Title: Medicine (Baltimore) Volume: 97 Issue: 23 Pages: e11077-e11077
  • DOI: 10.1097/md.0000000000011077
  • Peer Reviewed / Open Access
• [Journal Article] Epigenetic inactivation of galanin receptors in salivary duct carcinoma of the parotid gland: Potential utility as biomarkers for prognosis (2018)
  • Author(s): Kanazawa Takeharu、Misawa Kiyoshi、Fukushima Hirofumi、Misawa Yuki、Sato Yukiko、Maruta Mikiko、Imayoshi Shoichiro、Kusaka Gen、Kawabata Kazuyoshi、Mineta Hiroyuki、Carey Thomas、Nishino Hiroshi
  • Journal Title: Oncology Letters Volume: 15 Pages: 904-9050
  • DOI: 10.3892/ol.2018.8525
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The high expression of FOXA1 is correlated with a favourable prognosis in salivary duct carcinomas: a study of 142 cases (2018)
  • Author(s): Urano Makoto、Hirai Hideaki、Tada Yuichiro、Otsuka Kuninori、Watanabe Yoshihiro、Sakai Akihiro、Ebisumoto Koji、Togashi Takafumi、Ueki Yushi、Ota Hisayuki、Sato Yukiko、Saigusa Natsuki、Nakaguro Masato、Hanazawa Toyoyuki、Nagao Toshitaka
  • Journal Title: Histopathology Volume: 73 Issue: 6 Pages: 943-952
  • DOI: 10.1111/his.13706
  • Peer Reviewed / Open Access
• [Journal Article] MYB and MYBL1 in adenoid cystic carcinoma: diversity in the mode of genomic rearrangement and transcripts. (2018)
  • Author(s): Togashi Y, Dobashi A, Sakata S, Sato Y, Baba S, Seto A, Mitani H, Kawabata K, Takeuchi K
  • Journal Title: Modern pathology Volume: 印刷中 Issue: 6 Pages: 934-946
  • DOI: 10.1038/s41379-018-0008-8
  • Peer Reviewed
• [Journal Article] Hyalinizing clear cell carcinoma of the bronchial glands: presentation of three cases and pathological comparisons with salivary gland counterparts and bronchial mucoepidermoid carcinomas. (2018)
  • Author(s): Takamatsu M, Sato Y, Muto M, Nagano H, Ninomiya H, Sakakibara R, Baba S, Sakata S, Takeuchi K, Okumura S, Ishikawa Y
  • Journal Title: Modern pathology Volume: 印刷中 Issue: 6 Pages: 923-933
  • DOI: 10.1038/s41379-018-0025-7
  • Peer Reviewed
• [Journal Article] Prognostic and histogenetic roles of gene alteration and the expression of key potentially actionable targets in salivary duct carcinomas (2017)
  • Author(s): Shimura Tomotaka、Tada Yuichiro、Hirai Hideaki、Sato Yukiko、et al.
  • Journal Title: Oncotarget Volume: 9 Issue: 2 Pages: 1852-1867
  • DOI: 10.18632/oncotarget.22927
  • Peer Reviewed / Open Access
• [Journal Article] Biomarker immunoprofile in salivary duct carcinomas: clinicopathological and prognostic implications with evaluation of the revised classification (2017)
  • Author(s): Takase Soichiro、Kano Satoshi、Tada Yuichiro、Sato Yukiko、et al.
  • Journal Title: Oncotarget Volume: 8 Issue: 35 Pages: 59023-59035
  • DOI: 10.18632/oncotarget.19812
  • Peer Reviewed / Open Access
• [Journal Article] Impact of hematological inflammatory markers on clinical outcome in patients with salivary duct carcinoma: a multi-institutional study in Japan. (2017)
  • Author(s): Kawakita D, Tada Y, Imanishi Y, Beppu S, Tsukahara K, Kano S, Ozawa H, Okami K, Sato Y, Shimizu A, Sato Y, Fushimi C, Takase S, Okada T, Sato H, Otsuka K, Watanabe Y, Sakai A, Ebisumoto K, Togashi T, Ueki Y, Ota H, Shimura T, Hanazawa T, Murakami S, Nagao T.
  • Journal Title: Oncotarget Volume: 8(1) Issue: 1 Pages: 1083-1091
  • DOI: 10.18632/oncotarget.13565
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Central mucoepidermoid carcinoma arising from glandular odontogenic cyst confirmed by analysis of MAML2 rearrangement: A case report (2017)
  • Author(s): Nagasaki Atsuhiro、Ogawa Ikuko、Sato Yukiko、Takeuchi Kengo、Kitagawa Masae、Ando Toshinori、Sakamoto Shinnichi、Shrestha Madhu、Uchisako Kaori、Koizumi Koichi、Toratani Shigeaki、Konishi Masaru、Takata Takashi
  • Journal Title: Pathology International Volume: 68 Issue: 1 Pages: 31-35
  • DOI: 10.1111/pin.12609
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Establishment and characterization of a clear cell odontogenic carcinoma cell line with EWSR1-ATF1 fusion gene. (2017)
  • Author(s): Kujiraoka S, Tsunematsu T, Sato Y, Yoshida M, Tohyama R, Tanaka M, Kobayashi Y, Kondo T, Ushio A, Otsuka K, Kurosawa M, Saito M. Yamada A, Arakaki R, Nagai H, Nikai H, Takeuchi K, Nagao T, Miyamoto Y, Ishimaru N, Kudo Y.
  • Journal Title: Oral Oncol. Volume: 69 Pages: 46-55
  • DOI: 10.1016/j.oraloncology.2017.04.003
  • Peer Reviewed / Open Access
• [Presentation] 融合遺伝子を検出したHyalinizing clear cell carcinoma(HCCC)10症例の臨床的検討 (2022)
  • Author(s): 森田琢磨、佐藤由紀子, 福島啓文, 三谷浩樹, 佐々木徹 , 新橋渉 , 瀬戸陽 , 小泉雄 , 神山亮介 , 市川千恭 , 鳥居淳一 , 垣内晃人 , 檜原浩介
  • Organizer: 第46回頭頚部癌学会
• [Presentation] 急速な転帰を辿ったhigh-grade transformationを伴う口蓋腺様嚢胞癌の1例 (2022)
  • Author(s): 原口和也, 土生学, 矢田直美, 佐藤由紀子, 古田功彦, 冨永和宏.
  • Organizer: 第40回日本口腔腫瘍学会
• [Presentation] 唾液腺導管癌における自律神経(交感神経・副交感神経)の臨床・分子病理学的意義 (2022)
  • Author(s): 梶原真奈美, 高橋秀聡 , 川北大介, 平井秀明, 内海由貴, 浦野誠 , 佐藤由紀子, 塚原清彰 , 加納里志 , 大上研二 , 小澤宏之 , 富樫孝文, 岡田拓朗, 多田雄一郎, 長尾俊孝
  • Organizer: 第66回日本唾液腺学会
• [Presentation] Signet-ring cellの出現を伴った口蓋粘液腺癌の1例 (2022)
  • Author(s): 矢田直美 矢田直美, 小川郁子, 佐藤由紀子 , 宮内睦美 , 長尾俊孝 , 坂口修 , 松尾拡
  • Organizer: 第66回日本唾液腺学会
• [Presentation] 術前に腺房細胞癌が除外できなかった耳下腺オンコサイトーマの1例 (2019)
  • Author(s): 佐藤 由紀子, 神山 亮介, 瀬戸 陽, 佐々木 徹, 福島 啓文, 米川 博之, 山本 智理子, 三谷 浩樹, 竹内 賢吾
  • Organizer: 第 30 回日本臨床口腔病理学会
• [Presentation] 唾液腺導管癌における脂質関連蛋白発現の臨床病理学的意義 (2019)
  • Author(s): 平井 秀明, 多田 雄一郎, 中黒 匡人, 川北 大介, 佐藤 由紀子, 志村 智隆, 塚原 清彰, 加納 里志, 安藤 瑞生, 浦野 誠, 花澤 豊行, 長尾 俊孝
  • Organizer: 第64回日本唾液腺学会
• [Presentation] 口蓋に発生したhigh-grade transformationを伴う腺様嚢胞癌の1例 (2019)
  • Author(s): 矢田 直美, 佐藤 由紀子, 竹内 賢吾, 小川 郁子, 平林 文香, 吉賀 大午, 大谷 泰志, 松尾 拡
  • Organizer: 第64回日本唾液腺学会
• [Presentation] 頭頚部領域における臨床へのfeedback (2018)
  • Author(s): 佐藤由紀子
  • Organizer: 日本臨床細胞学会
• [Presentation] 頭頸部腺様嚢胞癌 : 予後因子の解析と、遠隔転移例に対する局所治療についての検討 (2018)
  • Author(s): 瀬戸 陽 、佐藤由紀子、冨樫由紀、土橋映仁、坂田征士 、馬場郷子、蛯名 彩 、竹内賢吾、 三谷浩樹
  • Organizer: 日本耳鼻咽喉科学会
• [Presentation] Clear cell-rich salivary gland carcinoma of the buccal mucosa (2018)
  • Author(s): 星野 都, 井上 勝元, 井上 ハルミ, 菊池 建太郎, 山内 雅司, 坂下 英明, 佐藤 由紀子, 竹内 賢吾, 長尾 俊孝, 草間 薫
  • Organizer: 日本病理学会
• [Presentation] FISH法にてEWSR1‐ATF1を検出したhyalinizing clear cell carcinoma(HCCC)5症例の臨床病理学的検討 (2017)
  • Author(s): 西嶌嘉容, 福島啓文, 佐藤由紀子, 三谷浩樹, 米川博之, 佐々木徹, 新橋渉, 瀬戸陽, 小泉雄, 神山亮介, 白尾浩太郎, 日高竜太, 服部雅優, 高橋剛史, 坂井利彦
  • Organizer: 第41回日本頭頸部癌学会
• [Presentation] 唾液腺導管癌におけるGalanin受容体のメチル化解析 金澤丈治, 福島啓文, 三澤清, 佐藤由紀子, 西野宏, 峯田周幸, 川端一嘉 (2017)
  • Author(s): 金澤丈治, 福島啓文, 三澤清, 佐藤由紀子, 西野宏, 峯田周幸, 川端一嘉
  • Organizer: 第41回日本頭頸部癌学会
• [Presentation] 腺様嚢胞癌100例の検討:組織病理学,分子生物学,バイオインフォマティクスを用いた統合的解析 (2017)
  • Author(s): 冨樫由紀, 土橋映仁, 坂田征士, 佐藤由紀子, 馬場郷子, 蛯名彩, 瀬戸陽, 三谷浩樹, 川端一嘉, 竹内賢吾, 竹内賢吾
  • Organizer: 第41回日本頭頸部癌学会
• [Presentation] 耳下腺導管部に発生した乳腺相似分泌癌の一例 (2017)
  • Author(s): 森田圭一, 栢森高, 名生邦彦, 道泰之, 山口聰, 佐藤由紀子, 竹内賢吾
  • Organizer: 第41回日本頭頸部癌学会
• [Presentation] 乳腺充実型腺様嚢胞癌と考えられた一例 (2017)
  • Author(s): 内田士朗, 鈴木高祐, 宇野美恵子, 野嵜史, 阿部江利子, 林透, 佐藤由紀子, 竹内賢吾, 秋山太
  • Organizer: 第106回日本病理学会
• [Presentation] 当科で経験したMammary analogue secretory carcinomaの2例 (2017)
  • Author(s): 市川千恭, 佐藤由紀子, 小幡和史, 日高竜太, 新橋渉, 佐々木徹, 福島啓文, 米川博之, 三谷浩樹, 川端一嘉
  • Organizer: 第27回日本頭頸部外科学会
• [Presentation] 長期経過後に両側腎,肺転移を来した副咽頭間隙の転移性多形腺腫の1例 (2017)
  • Author(s): 佐藤由紀子, 山田麻里沙, 鈴木奈緒子, 池畑浩一, 星利良, 藤山淳三, 古田則行, 小松京子, 小松京子, 杉山裕子, 石川雄一
  • Organizer: 第56回日本臨床細胞学会秋季大会