| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We investigated which genetic alterations observed in human colorectal carcinogenesis are critical in the inflammation-related bowel carcinogenesis. The inflammation was evoked by implantation of foreign substances such as plastic plate. Male mouse intestinal organoids with K-ras activation and APC knockdown were grew lethally in the inflammation formed in female nude mice. When an organoid obtained from a homozygous p53 gene-deficient mouse and implanted them into the inflammatory region, they formed fibrous tumors. Organoids obtained from a heterozygous p53 gene-deficient mouse, they did not grow under the inflammation. Organoid obtained from a p53 gene homozygous mouse with APC knockdown formed tumors in mice, however, the histological type were fibrous ones.
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