| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
To elucidate the physiological function of HPD1 highly expressed in fat-accumulated cells, we prepared HPD1-knockdown adipocytes (3T3-L1 cells). Genechip analysis was used to analyze the gene expression of HPD1 knockdown and control cell mRNAs. The genes causing change in the expression by the knockdown of HPD1 was lipid metabolism, glucose metabolism, cytokine receptor and cytoskeleton-related genes. In these genes, hexokinase (known as glucose kinase) was focused. The hexokinase in HPD1-knockdown adipocytes led to the significant decrease of mRNA and protein levels and also glucose kinase activity compared to that in control adipocytes. The subcellular localization of HPD1 was previously demonstrated as detectable proteins in the cytoskeleton-enriched fraction. These results suggest that HPD1 in adipocytes indirectly associates with transcriptional regulation of a large number of genes.
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