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Extensive exploration of Helicobacter pylori virulence genes using next generation sequencer

Research Project

Project/Area Number 17K08834
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including mycology)
Research InstitutionOita University

Principal Investigator

Suzuki Rumiko  大分大学, 医学部, 客員研究員 (70599092)

Co-Investigator(Kenkyū-buntansha) 山岡 吉生  大分大学, 医学部, 教授 (00544248)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsピロリ菌 / ゲノム解析 / 病原遺伝子 / 系統関係 / 人類移動 / ゲノムシーケンス / 系統解析 / 次世代シーケンサー / 胃がん / 胃MALTリンパ腫 / Helicobacter pylori / 疾患関連遺伝子 / 次世代シーケンシング / バイオインフォマティクス
Outline of Final Research Achievements

This study compared genome sequences of Helicobacter pylori read by next generation sequencers to explore new virulence genes among East Asian strains. We identified difference of genes between gastric adenocarcinoma and MALT lymphoma other than known virulence genes. We also compared H. pylori genomes before and after inoculation to Mongolian gerbils, which is a model animal of infection, and identified mutations during the incubation in the animal stomach. Our preceding study observed weak virulence strains in Okinawa, however, its origin was unknown. This study discovered that the Okinawa strain originate from Central Asia and that it reached to Okinawa around 30,000 years ago.

Academic Significance and Societal Importance of the Research Achievements

東アジア型のピロリ菌は、大部分が強毒型の既知病原遺伝子を持っており、それが胃がん発症率の高さにつながっている。しかし、同じ強毒型のピロリ菌に感染しても発症する疾患が同じとは限らず、既知病原遺伝子だけでは疾患の違いが説明できなかった。我々は胃がん株と胃MALTリンパ腫株のゲノム比較から遺伝子の新規な差異を見出し、疾患の違いにつながる因子の手がかりを得た。また、モデル動物への適応に伴う遺伝子の変化も同定した。系統解析からは弱毒型の沖縄株が中央アジアに起源を持つことを明らかにし、先史時代における日本への人類移動に関しても新たな視点を加える結果を得た。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (7 results)

All 2019 2018 2017

All Journal Article (4 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (3 results)

  • [Journal Article] Genome-wide mutation analysis of Helicobacter pylori after inoculation to Mongolian gerbils2019

    • Author(s)
      Suzuki Rumiko、Satou Kazuhito、Shiroma Akino、Shimoji Makiko、Teruya Kuniko、Matsumoto Takashi、Akada Junko、Hirano Takashi、Yamaoka Yoshio
    • Journal Title

      Gut Pathogens

      Volume: 11 Issue: 1 Pages: 1-6

    • DOI

      10.1186/s13099-019-0326-5

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Helicobacter pylori virulence genes of minor ethnic groups in North Thailand2017

    • Author(s)
      Subsomwong Phawinee、Miftahussurur Muhammad、Vilaichone Ratha-korn、Ratanachu-ek Thawee、Suzuki Rumiko、Akada Junko、Uchida Tomohisa、Mahachai Varocha、Yamaoka Yoshio
    • Journal Title

      Gut Pathogens

      Volume: 9 Issue: 1 Pages: 56-60

    • DOI

      10.1186/s13099-017-0205-x

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Rapid evolution of distinct Helicobacter pylori subpopulations in the Americas.2017

    • Author(s)
      Thorell K, Yahara K, Berthenet E, Lawson DJ, Mikhail J, Kato I, Mendez A, Rizzato C, Bravo MM, Suzuki R, Yamaoka Y, Torres J, Sheppard SK, Falush D.
    • Journal Title

      PLoS Genet

      Volume: 13(2) Issue: 2 Pages: 1-21

    • DOI

      10.1371/journal.pgen.1006546

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Two populations of less-virulent Helicobacter pylori genotypes in Bangladesh2017

    • Author(s)
      Aftab Hafeza、Miftahussurur Muhammad、Subsomwong Phawinee、Ahmed Faruque、Khan A. K. Azad、Matsumoto Takashi、Suzuki Rumiko、Yamaoka Yoshio
    • Journal Title

      PLOS ONE

      Volume: 12 Issue: 8 Pages: 1-17

    • DOI

      10.1371/journal.pone.0182947

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ピロリ菌から探る旧石器時代の人類移動ユーラシア大陸から日本列島へ2019

    • Author(s)
      鈴木留美子
    • Organizer
      日本遺伝学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 旧石器時代人類の内陸移動 Helicobacter pyloriからの推測2018

    • Author(s)
      鈴木留美子、山岡吉生、斎藤成也、平野隆、佐藤万仁
    • Organizer
      日本進化学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 沖縄特異的なヘリコバクター・ピロリの由来を探る2017

    • Author(s)
      鈴木留美子,松成修,斎藤成也,山岡吉生
    • Organizer
      日本進化学会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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