| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Annexins (ANXs) comprise a family of calcium- and phospholipid-binding proteins and are implicated to involve in not only a numerous of cellular processes but also life-cycle of several viral infections. Here we demonstrate a novel insight of annexin A5 (ANXA5) on the HCV life-cycles. The knockdown of ANXA5 remarkably increased the expression of both HCV proteins and HCV RNA levels in the HCV replicon cells. Using an infectious HCV clones, we confirmed the significant enhancement of HCV propagation in the ANXA5 knockdown cells, suggesting that ANXA5 acts as a negative regulator of HCV infection. Furthermore, the confocal microscopic analysis revealed that ANXA5 is required for the proper cellular localization of tight-junction (TJ) proteins such as CLDN-1 and OCLN, which are critical entry factors for HCV infection. These results provide new insights into the HCV life-cycle on the function of ANXA5 that acts via modulation of TJ proteins assembly.
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