| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
We generated genome-wide human gene knockout library in Safford virus (SAFV) highly susceptible HeLa cells using the CRISPR/Cas9 system and conducted a search for SAFV receptors. As a result, we obtained three candidate genes that are thought to be receptors or promote viral infection and replication. We are currently analyzing these genes for their functions in SAFV infection, focusing on membrane proteins. We also generated a fluorescent protein-expressing recombinant SAFV that can easily detect viral infection.
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