| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
The thymus is an important organ for T cells to establish self-tolerance and non-self responsiveness. Aire expressed in thymic medullary epithelial cells (mTECs) plays an important role in the expression of various antigens in mTEC. In addition to Aire knockout mice, we analyzed mTEC gene expression in mice that over-express Aire (huAIRE transgenic mouse, 3xAire knock-in mouse). We identified the factors that down-regulate in mTECs from both Aire knockout mice and Aire overexpressing mice. We analyzed "negative selection" in the model-system which employed OT-II and RIP-OVA transgenic mice, "negative selection" was also impaired in Aire over-expressing mice. We have newly categorized the regulation of gene expression by Aire.
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