Establishment of detection methods of early cancers with chronic inflammation by non-viral mechanism

• Project/Area Number: 17K09004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Chiba University
• Principal Investigator: Matsushita Kazuyuki 千葉大学, 医学部附属病院, 准教授 (90344994)
• Co-Investigator(Kenkyū-buntansha): 西村 基 千葉大学, 医学部附属病院, 講師 (80400969) ; 星野 忠次 千葉大学, 大学院薬学研究院, 准教授 (90257220)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: がんバイオマーカー / 慢性炎症 / 発がん / 慢性炎症刺激 / 発癌動物モデル / 自己抗体 / FIRΔexon2 / ECLIA法 / ドットブロット / 消化器癌 / AlphaLisa / EIA / イムノアッセイ / スプライシング変異体 / 動物モデル / 非ウイルス性慢性炎症 / バイオマーカー / がん治療 / がん診断 / スプライシング変異 / マウスモデル / Wntシグナル / 発がんメカニズム / 早期がんの診断

Outline of Final Research Achievements

FIR, a splicing variant of the PUF60 gene, is a transcriptional repressor of c-myc. Previously, large-scale SEREX (serological identification of antigens by recombinant cDNA expression cloning) screenings have identified anti-FIR auto-antibodies in the sera of cancer patients. In the present study, we revealed the presence and significance of anti-FIR (FIR/FIRΔexon2) antibodies (Abs) in the sera of patients with gastric cancer (GC). Our results were validated by performing an Alpha-LISA (Amplified Luminescence Proximity Homogeneous Assay) using sera of patients with various cancers types. We revealed that anti-FIRΔexon2 Ab had higher sensitivity than anti-FIR Ab. ROC (receiver operating curve) analysis was performed for evaluating the use of anti-FIRΔexon2 Ab as candidate markers such as CEA and CA19-9, and the highest area under the ROC analysis was observed in combination of anti-FIRΔexon2 Ab and CEA.

Academic Significance and Societal Importance of the Research Achievements

本研究では慢性炎症刺激による発癌のカギ分子となる候補として、FIRの機能異常に着目して検討を行った。種々の癌患者（千葉大学医学部附属病院通院中）の血清中のFIRΔexon2に対する自己抗体が検出されることを確認し、新しい癌診断法の開発を行った。FIRΔexon2（513アミノ酸）の全長タンパク質を抗原を精製した。癌患者と健常者血清を用いて、抗FIRΔexon2自己抗体が検出可能であり論文報告した。ヒト胃癌組織ではp-ERK, FBW7,　E-cadherinの発現変化が炎症から癌化への形質転換に重要であることが強く示唆された。

Research Products

• [Journal Article] Anti-FIRΔexon2 autoantibody as a novel indicator for better overall survival in gastric cancer (2021)
  • Author(s): Kobayashi S, Hiwasa T, Ishige T, Kano M, Hoshino T, Rahmutulla B, Seimiya M, Shimada H, Nomura F, Matsubara H, Matsushita K.
  • Journal Title: Cancer Science Volume: 112 Issue: 2 Pages: 847-858
  • DOI: 10.1111/cas.14767
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Post-transcriptional regulation of BRG1 by FIRΔexon2 in gastric cancer (2020)
  • Author(s): Ailiken Guzhanuer、Kitamura Kouichi、Hoshino Tyuji、Satoh Mamoru、Tanaka Nobuko、Minamoto Toshinari、Rahmutulla Bahityar、Kobayashi Sohei、Kano Masayuki、Tanaka Tomoaki、Kaneda Atsushi、Nomura Fumio、Matsubara Hisahiro、Matsushita Kazuyuki
  • Journal Title: Oncogenesis Volume: 9 Issue: 2 Pages: 26-26
  • DOI: 10.1038/s41389-020-0205-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An in-house centrifugation and membrane filtration technique for identifying microorganisms from positive blood culture bottles with high identification rates using matrix-assisted laser desorption ionization?Time-of-flight mass spectrometry: A preliminary report (2020)
  • Author(s): Tsuchida Sachio、Murata Syota、Miyabe Akiko、Satoh Mamoru、Takiwaki Masaki、Matsushita Kazuyuki、Nomura Fumio
  • Journal Title: Journal of Infection and Chemotherapy Volume: 26 Issue: 3 Pages: 266-271
  • DOI: 10.1016/j.jiac.2019.09.017
  • Peer Reviewed / Open Access
• [Journal Article] MT Seminar これだけはおさえておきたい免疫チェックポイント阻害剤 (2020)
  • Author(s): 松下 一之
  • Journal Title: Medical Technology 48(2) 198 - 205 2020年2月 Volume: 48 Pages: 198-205
  • Peer Reviewed / Open Access
• [Journal Article] Diagnostic Challenges Posed by Preceding Peripheral Neuropathy in Very Late-onset Spinocerebellar Ataxia Type 3 (2019)
  • Author(s): Sugiyama A, Sekiguchi Y, Beppu M, Ishige T, Matsushita K, Kuwabara S.
  • Journal Title: Internal Medicine Volume: 58 Issue: 1 Pages: 119-122
  • DOI: 10.2169/internalmedicine.1382-18
  • NAID: 130007542250
  • ISSN: 0918-2918, 1349-7235
  • Year and Date: 2019-01-01
  • Peer Reviewed / Open Access
• [Journal Article] Central blood pressure and pulse wave velocity in young and middle-aged Japanese adults with isolated systolic hypertension (2019)
  • Author(s): Nakagomi Atsushi、Imazeki Fumio、Nishimura Motoi、Sawabe Yuji、Matsushita Kazuyuki、Murata Akitoshi、Watase Mariko、Okada Sho、Kobayashi Yoshio
  • Journal Title: Hypertension Research Volume: 43 Issue: 3 Pages: 207-212
  • DOI: 10.1038/s41440-019-0364-x
  • Peer Reviewed / Open Access
• [Journal Article] A simple method for sequencing the whole human mitochondrial genome directly from samples and its application to genetic testing (2019)
  • Author(s): Yao Yue、Nishimura Motoi、Murayama Kei、Kuranobu Naomi、Tojo Satomi、Beppu Minako、Ishige Takayuki、Itoga Sakae、Tsuchida Sachio、Mori Masato、Takayanagi Masaki、Yokoyama Masataka、Yamagata Kazuyuki、Kishita Yoshihito、Okazaki Yasushi、Nomura Fumio、Matsushita Kazuyuki、Tanaka Tomoaki
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 17411-17421
  • DOI: 10.1038/s41598-019-53449-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Multiplex PCR and multicolor probes melting for the simultaneous detection of five UGT1A1 variants (2019)
  • Author(s): Ishige Takayuki、Itoga Sakae、Kawasaki Kenji、Sawai Setsu、Nishimura Motoi、Nomura Fumio、Matsushita Kazuyuki
  • Journal Title: Analytical Biochemistry Volume: 587 Pages: 113448-113448
  • DOI: 10.1016/j.ab.2019.113448
  • Peer Reviewed / Open Access
• [Journal Article] Anti-FIRΔexon2, a splicing variant form of PUF60, auto-antibody is detected in the sera of esophageal squamous cell carcinoma. (2019)
  • Author(s): Kobayashi S, Hiwasa T, Ishige T, Rahmutulla B, Kano M, Hoshino T, Minamoto T, Shimada H, Nomura F, Matsubara H, Matsushita K.
  • Journal Title: Cancer Sci. Volume: 4 Issue: 6 Pages: 1-10
  • DOI: 10.1111/cas.14024
  • Peer Reviewed / Open Access
• [Journal Article] [Dawn of Cancer Clinical Sequencing in Chiba University Hospital]. (2018)
  • Author(s): Kano M, Miyauchi H, Matsushita K, Murakami K, Toyozumi T, Hayano K, Otsuka R, Takahashi M, Sekino N, Shiraishi T, Arai M, Takiguchi Y, Matsubara H.
  • Journal Title: Gan To Kagaku Ryoho. Volume: 45 Pages: 1463-1465
  • Peer Reviewed / Open Access
• [Journal Article] High-throughput genotyping of GC (vitamin D-binding protein) by melting analysis with locked nucleic acid-incorporating dual hybridization probe for improving mismatch discrimination. (2018)
  • Author(s): Ishige T, Satoh M, Itoga S, Nishimura M, Matsushita K, Nomura F.
  • Journal Title: Clin Chim Acta Volume: 487 Pages: 126-132
  • DOI: 10.1016/j.cca.2018.09.034
  • Peer Reviewed / Open Access
• [Journal Article] The frequency of promoter DNA hypermethylation is decreased in colorectal neoplasms of familial adenomatous polyposis. (2018)
  • Author(s): Takane K, Fukuyo M, Matsusaka K, Ota S, Rahmutulla B, Matsushita K, Miyauchi H, Nakatani Y, Matsubara H, Kaneda A.
  • Journal Title: Oncotarget. Volume: 9 Issue: 66 Pages: 32653-32666
  • DOI: 10.18632/oncotarget.25987
  • Peer Reviewed / Open Access
• [Journal Article] Application of the biocopolymer preparation system, rapid BACproII kit, for mass-spectrometry-based bacterial identification from positive blood culture bottles by the MALDI Biotyper system. (2018)
  • Author(s): Tsuchida S, Murata S, Miyabe A, Satoh M, Takiwaki M, Ashizawa K, Terada T, Ito D, Matsushita K, Nomura F.
  • Journal Title: J Microbiol Methods. Volume: 152 Pages: 86-91
  • DOI: 10.1016/j.mimet.2018.07.017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Assessing the Accuracy of Variant Detection in Cost-Effective Gene Panel Testing by Next-Generation Sequencing. (2018)
  • Author(s): Fujiki R, Ikeda M, Yoshida A, Akiko M, Yao Y, Nishimura M, Matsushita K, Ichikawa T, Tanaka T, Morisaki H, Morisaki T, Ohara O.
  • Journal Title: J Mol Diagn. Volume: 96 Issue: 5 Pages: 572-582
  • DOI: 10.1016/j.jmoldx.2018.04.004
  • Peer Reviewed / Open Access
• [Journal Article] Molecular Nodal Staging Using miRNA Expression in Lung Cancer Patients by Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration (2018)
  • Author(s): Inage Terunaga、Nakajima Takahiro、Itoga Sakae、Ishige Takayuki、Fujiwara Taiki、Sakairi Yuichi、Wada Hironobu、Suzuki Hidemi、Iwata Takekazu、Chiyo Masako、Yoshida Shigetoshi、Matsushita Kazuyuki、Yasufuku Kazuhiro、Yoshino Ichiro
  • Journal Title: Respiration Volume: 96 Issue: 3 Pages: 267-274
  • DOI: 10.1159/000489178
  • Peer Reviewed / Open Access
• [Journal Article] Investigation of novel biomarkers for predicting the clinical course in patients with ulcerative colitis (2018)
  • Author(s): Hamanaka Shinsaku、Nakagawa Tomoo、Hiwasa Takaki、Ohta Yuki、Kasamatsu Shingo、Ishigami Hideaki、Taida Takashi、Okimoto Kenichiro、Saito Keiko、Maruoka Daisuke、Matsumura Tomoaki、Takizawa Hirotaka、Kashiwado Koichi、Kobayashi Sohei、Matsushita Kazuyuki、Matsubara Hisahiro、Katsuno Tatsuro、Arai Makoto、Kato Naoya
  • Journal Title: Journal of Gastroenterology and Hepatology Volume: 33 Issue: 12 Pages: 1975-1983
  • DOI: 10.1111/jgh.14297
  • Peer Reviewed / Open Access
• [Journal Article] Disturbed alternative splicing of FIR (PUF60) directed cyclin E overexpression in esophageal cancers (2018)
  • Author(s): Ogura Yukiko、Hoshino Tyuji、Tanaka Nobuko、Ailiken Guzhanuer、Kobayashi Sohei、Kitamura Kouichi、Rahmutulla Bahityar、Kano Masayuki、Murakami Kentarou、Akutsu Yasunori、Nomura Fumio、Itoga Sakae、Matsubara Hisahiro、Matsushita Kazuyuki
  • Journal Title: Oncotarget Volume: 9 Issue: 33 Pages: 22929-22944
  • DOI: 10.18632/oncotarget.25149
  • Peer Reviewed / Open Access
• [Journal Article] Identification of specific and common diagnostic antibody markers for gastrointestinal cancers by SEREX screening using testis cDNA phage library (2018)
  • Author(s): Kobayashi Sohei、Hiwasa Takaki、Arasawa Takahiro、Kagaya Akiko、Ishii Sayaka、Shimada Hideaki、Ito Masaaki、Suzuki Masae、Kano Masayuki、Rahmutulla Bahityar、Kitamura Kouichi、Sawabe Yuji、Shin Hideo、Takiguchi Masaki、Nomura Fumio、Matsubara Hisahiro、Matsushita Kazuyuki
  • Journal Title: Oncotarget Volume: 9 Issue: 26 Pages: 18559-18569
  • DOI: 10.18632/oncotarget.24963
  • Peer Reviewed / Open Access
• [Journal Article] Multi-panel assay of serum autoantibodies in colorectal cancer (2018)
  • Author(s): Ushigome Mitsunori、Nabeya Yoshihiro、Soda Hiroaki、Takiguchi Nobuhiro、Kuwajima Akiko、Tagawa Masatoshi、Matsushita Kazuyuki、Koike Junichi、Funahashi Kimihiko、Shimada Hideaki
  • Journal Title: International Journal of Clinical Oncology Volume: 23 Issue: 5 Pages: 917-923
  • DOI: 10.1007/s10147-018-1278-3
  • Peer Reviewed / Open Access
• [Journal Article] An improved in-house lysis-filtration protocol for bacterial identification from positive blood culture bottles with high identification rates by MALDI-TOF MS. (2018)
  • Author(s): Tsuchida S, Murata S, Miyabe A, Satoh M, Takiwaki M, Matsushita K, Nomura F
  • Journal Title: Journal of microbiological methods Volume: 18 Pages: 1684-1688
  • DOI: 10.1016/j.mimet.2018.03.014
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Capsular serotyping of Haemophilus influenzae by using matrix-associated laser desorption ionization-time of flight mass?spectrometry. (2018)
  • Author(s): Takeuchi N, Segawa S, Ishiwada N, Ohkusu M, Tsuchida S, Satoh M, Matsushita K, Nomura F
  • Journal Title: Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy Volume: 18 Issue: 7 Pages: 300062-66
  • DOI: 10.1016/j.jiac.2018.02.007
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Surveillance evaluation of the standardization of assay values for serum total 25-hydroxyvitamin D concentration in Japan. (2018)
  • Author(s): Ihara H, Kiuchi S, Ishige T, Nishimura M, Matsushita K, Satoh M, Nomura F, Yamashita M, Kitajima I, Tsugawa N, Okano T, Hirota K, Miura M, Totani M, Hashizume N; Nutrition Division, Japan Society of Clinical Chemistry.
  • Journal Title: Ann Clin Biochem. Volume: Epub ahead of print Issue: 6 Pages: 0-0
  • DOI: 10.1177/0004563218765570
  • Peer Reviewed / Open Access
• [Journal Article] Assessment by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of the Effects of Preanalytical Variables on Serum Peptidome Profiles Following Long-Term Sample Storage. (2018)
  • Author(s): Tsuchida S, Satoh M, Umemura H, Sogawa K, Takiwaki M, Ishige T, Miyabayashi Y, Iwasawa Y, Kobayashi S, Beppu M, Nishimura M, Kodera Y, Matsushita K, Nomura F
  • Journal Title: Proteomics. Clinical applications Volume: 15 Issue: 3 Pages: 47-53
  • DOI: 10.1002/prca.201700047
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Variant in C-terminal region of intestinal alkaline phosphatase associated with benign familial hyperphosphatasaemia. (2018)
  • Author(s): Ishige T, Itoga S, Utsuno E, Nishimura M, Yoshikawa M, Kato N, Matsushita K, Yokosuka O, Nomura F
  • Journal Title: Journal of medical genetics Volume: 10 Issue: 10 Pages: 47-53
  • DOI: 10.1136/jmedgenet-2017-104964
  • Peer Reviewed / Open Access
• [Journal Article] Locked Nucleic Acid Technology for Highly Sensitive Detection of Somatic Mutations in Cancer. (2018)
  • Author(s): Ishige T, Itoga S, Matsushita K
  • Journal Title: Advances in clinical chemistry Volume: 83 Pages: 53-72
  • DOI: 10.1016/bs.acc.2017.10.002
  • Peer Reviewed / Open Access
• [Journal Article] Improved sensitivity of serum/plasma 1α,25-dihydroxyvitamin D quantification by DAPTAD derivatization (2017)
  • Author(s): Ishige Takayuki, Satoh Mamoru, Ogawa Shoujiro, Nishimura Motoi, Matsushita Kazuyuki, Higashi Tatsuya, Nomura Fumio
  • Journal Title: Clinica Chimica Acta Volume: 473 Pages: 173-179
  • DOI: 10.1016/j.cca.2017.08.033
  • Peer Reviewed / Open Access
• [Journal Article] Applications of copolymer for rapid identification of bacteria in blood culture broths using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. (2017)
  • Author(s): Ashizawa K, Murata S, Terada T, Ito D, Bunya M, Watanabe K, Teruuchi Y, Tsuchida S, Satoh M, Nishimura M, Matsushita K, Sugama Y, Nomura F
  • Journal Title: Journal of microbiological methods Volume: 139 Pages: 54-60
  • DOI: 10.1016/j.mimet.2017.04.013
  • Peer Reviewed / Open Access
• [Journal Article] FIR haplodeficiency promotes splicing to pyruvate kinase M2 (2017)
  • Author(s): A. Kimura, K.Kitamura, M. Satoh, K. Matsushita, et al.
  • Journal Title: Oncotarget Volume: 40 Issue: 40 Pages: 67955-67965
  • DOI: 10.18632/oncotarget.19061
  • Peer Reviewed / Open Access
• [Journal Article] A novel KCNQ1 nonsense variant in the isoform-specific first exon causes both jervell and Lange-Nielsen syndrome 1 and long QT syndrome 1: a case report. (2017)
  • Author(s): Nishimura M, Ueda M, Ebata R, Utsuno E, Ishii T, Matsushita K, Ohara O, Shimojo N, Kobayashi Y, Nomura F
  • Journal Title: BMC medical genetics Volume: 18 Issue: 1 Pages: 66-71
  • DOI: 10.1186/s12881-017-0430-7
  • Peer Reviewed / Open Access
• [Journal Article] Rapid and cost-effective high-throughput sequencing for identification of germline mutations of BRCA1 and BRCA2 (2017)
  • Author(s): Ahmadloo S, Nakaoka H, Hayano T, Hosomichi K, You H, Utsuno E, Sangai T, Nishimura M, Matsushita K, Hata A, Nomura F, Inoue I
  • Journal Title: J Hum Genetics Volume: 印刷中 Issue: 5 Pages: 561-567
  • DOI: 10.1038/jhg.2017.5
  • NAID: 40021210379
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 【臨床検査の最前線-将来の検査を展望する】 総論 臨床検査の将来 ゲノム解析技術の進歩と臨床検査への応用 (2017)
  • Author(s): 松下 一之
  • Journal Title: 医学のあゆみ Volume: 263 Pages: 991-998
  • Open Access
• [Journal Article] 国内のバイオバンクの概況・臨床検査からみたバイオバンクの意義 (iPSバンクを含む国内のバイオバンクの現状と課題) (2017)
  • Author(s): 松下 一之
  • Journal Title: 臨床病理 Volume: 65 Pages: 181-189
  • NAID: 40021119633
  • Peer Reviewed
• [Journal Article] 新規腫瘍マーカーと術後モニタリングマーカー候補としての消化器癌患者血清における抗FIRs自己抗体の検出 (2017)
  • Author(s): 小林 崇平, 松下 一之、日和佐 隆樹
  • Journal Title: 日本分子腫瘍マーカー研究会誌 Volume: 32 Pages: 39-39
  • NAID: 130005466730
• [Presentation] ゲノム医療新時代に向けて、病理部と臨床検査部の協調体制のあり方 がんゲノム医療における臨床検査部と病理部との連携 (2019)
  • Author(s): 松下 一之
  • Organizer: 臨床検査医学会
• [Presentation] 家族性腫瘍に対する千葉県内の診療施設間ネットワーク形成について (2019)
  • Author(s): 松下 一之, 石毛 崇之, 宮内 英聡, 糸賀 栄, 宇津野 恵美, 西村 基, 澤井 摂, 稲田 麻里, 横井 左奈, 中津川 智子, 三橋 暁, 小原 收, 野村 文夫, 市川 智彦
  • Organizer: 日本遺伝カウンセリング学会
• [Presentation] The significance of genetic testing for the genetic counseling to Lynch syndrome and the at-risk pre-symptomatic family members. (2019)
  • Author(s): Kazuyuki Matsushita and Takayuki Ishige.
  • Organizer: The International Society for Gastrointestinal Hereditary Tumours 2019. Kitchener Street, Auckland, New Zealand,March 21st, 2019.
• [Presentation] Lynch症候群に対する遺伝子関連検査と診療体制のための診療施設間ネットワーク形成について (2019)
  • Author(s): 松下一之, 石毛崇之, 糸賀 栄, 澤井 摂, 西村 基 ,中津川智子, 稲田麻里, 野村文夫, 内垣洋祐, 宇津野恵美, 小原 收, 市川智彦
  • Organizer: 第64回日本人類遺伝学会。2019.11.9 長崎新聞文化ホール。
• [Presentation] The significance of genetic testing for the genetic counseling to Lynch syndrome and the at-risk pre-symptomatic family members. (2019)
  • Author(s): Kazuyuki Matsushita and Takayuki Ishige
  • Organizer: The International Society for Gastrointestinal Hereditary Tumours
  • Int'l Joint Research
• [Presentation] Clinical tumor testing for precision cancer medicine ain laboratory medicine (2018)
  • Author(s): Kazuyuki Matsushita
  • Organizer: 2nd International Symposium of MEXT Program “Fostering Health Professionals for Changing Needs of Cancer” Precision Cancer Medicine
  • Int'l Joint Research / Invited
• [Presentation] 臨床検査からみたがんゲノム医療の実装と課題 (2018)
  • Author(s): 松下一之
  • Organizer: ちば胸部腫瘍カンファレンス
  • Invited
• [Presentation] Anti-FIRs (PUF60) auto-antibodies were detected in the sera of early-stage colon cancer patients. -Identification of specific and common diagnostic antibody markers for gastrointestinal cancers by SEREX screening using testis cDNA phage library (2018)
  • Author(s): Kazuyuki Matsushita and Sohei Kobayashi
  • Organizer: 3rd International Cancer Study & Therapy Conference.
  • Int'l Joint Research
• [Presentation] Anti-FIRs (PUF60) auto-antibodies are detected in the sera of early-stage colon cancer patients. (2018)
  • Author(s): Kazuyuki Matsushita
  • Organizer: Interenational conference on biomarker research in clinical medicine
  • Int'l Joint Research
• [Presentation] Anti-FIRs (PUF60) auto-antibodies are detected in the sera of early-stage colon cancer patients. (2018)
  • Author(s): Kazuyuki Matsushita and Sohei Kobayashi
  • Organizer: Biomarker Research in Clinical Medicine.
  • Int'l Joint Research
• [Presentation] microRNA発現定量解析による高感度肺癌リンパ節転移検出法の開発 (2017)
  • Author(s): 稲毛 輝長, 中島 崇裕, 藤原 大樹, 田中 教久, 坂入 祐一, 和田 啓伸, 鈴木 秀海, 岩田 剛和, 千代 雅子, 吉野 一郎, 松下 一之, 石毛 崇之, 糸賀 栄
  • Organizer: 千葉医学雑誌
• [Presentation] 血液培養陽性検体よりの複数菌由来の核酸検出 (2017)
  • Author(s): 西村 基, 村田 正太, 姚 躍, 土田 祥央, 田中 知明, 松下 一之
  • Organizer: 日本臨床微生物学雑誌
• [Presentation] 治療法のある遺伝性疾患に対する遺伝学的検査と遺伝カウンセリングネットワークの構築 (2017)
  • Author(s): 松下 一之
  • Organizer: 調査研究ジャーナル
  • Invited
• [Presentation] 大腸癌根治切除例におけるバイオマーカーとしてのRAS、BRAF、MSI statusに関する検討 (2017)
  • Author(s): 宮内 英聡, 大平 学, 加賀谷 暁子, 今西 俊介, 栃木 透, 丸山 哲郎, 松下 一之, 糸賀 栄, 石毛 崇之, 松原 久裕
  • Organizer: 日本癌治療学会学術集会抄録集
• [Presentation] 固形がん(非大腸癌)に対するMSI(microsatellite instability)検査の重要性と課題について (2017)
  • Author(s): 松下 一之, 石毛 崇之, 糸賀 栄, 西村 基, 澤部 祐司
  • Organizer: 臨床病理
  • Invited
• [Presentation] 治療法・診断法がある遺伝性がんに対する千葉県内における検診体制の確立 (2017)
  • Author(s): 松下 一之, 野村 文夫, 横井 左奈, 永瀬 浩喜, 市川 智彦
  • Organizer: 日本癌学会総会記事
• [Presentation] 家族性ポリポーシスの層別化 (2017)
  • Author(s): 高根 希世子, 松坂 恵介, 太田 聡, 福世 真樹, 西村 基, 酒井 英嗣, 松下 一之, 油谷 浩幸, 中谷 行雄, 松原 久裕, 赤木 究, 金田 篤志
  • Organizer: 日本癌学会総会記事
• [Presentation] 新規慢性肝疾患マーカーFIC5.9(Fibrinogen alpha C chain 5.9 kD fragment)は凝固線溶の変動を反映する (2017)
  • Author(s): 西村 基, 菊地 渉, 佐藤 守, 松下 一之, 小寺 義男, 田中 知明, 野村 文夫
  • Organizer: 臨床化学
• [Presentation] 生化学自動分析装置を用いたICG測定法(長波長対照法)の開発 (2017)
  • Author(s): 佐藤 有華, 清宮 正徳, 吉田 俊彦, 澤部 祐司, 松下 一之
  • Organizer: 日本医学検査学会抄録集
• [Presentation] 架橋化核酸を用いた高感度サンガー法によるRAS遺伝子変異解析 (2017)
  • Author(s): 石毛 崇之, 糸賀 栄, 松下 一之
  • Organizer: 日本医学検査学会抄録集
• [Presentation] 当科における家族性大腸ポリポーシスに対する診療状況 (2017)
  • Author(s): 大平 学, 宮内 英聡, 成島 一夫, 加賀谷 暁子, 武藤 頼彦, 斉藤 洋茂, 松原 久裕, 松下 一之
  • Organizer: 日本大腸肛門病学会雑誌
• [Presentation] メディカルチェックで先天性QT延長症候群が疑われたプロスポーツ選手に対する遺伝カウンセリングの1例 (2017)
  • Author(s): 内垣 洋祐, 西村 基, 上田 希彦, 岡田 将, 宇津野 恵美, 浦尾 充子, 別府 美奈子, 松下 一之, 野村 文夫, 長田 久夫, 羽田 明, 市川 智彦, 田中 知明, 山本 修一
  • Organizer: 日本遺伝カウンセリング学会誌
• [Presentation] リンチ症候群2家系における未発症家系員の発症前診断と課題 (2017)
  • Author(s): 松下 一之, 糸賀 栄, 石毛 崇之, 宇津野 恵美, 西村 基, 別府 美奈子, 内垣 洋祐, 野村 文夫, 澤部 祐司
  • Organizer: 日本遺伝カウンセリング学会誌
• [Presentation] 千葉大学医学部附属病院における遺伝性神経疾患の発症前診断の現状と問題点 (2017)
  • Author(s): 別府 美奈子, 宇津野 恵美, 浦尾 充子, 澤井 摂, 野村 文夫, 内垣 洋祐, 西村 基, 松下 一之, 桑原 聡, 田中 知明, 市川 智彦
  • Organizer: 日本遺伝カウンセリング学会誌
• [Presentation] microRNA発現定量解析による高感度肺癌リンパ節転移検出法の開発 (2017)
  • Author(s): 稲毛 輝長, 中島 崇裕, 藤原 大樹, 糸賀 栄, 石毛 崇之, 伊藤 祐輝, 小野里 優希, 松本 寛樹, 椎名 裕樹, 佐田 諭己, 畑 敦, 豊田 行英, 田中 教久, 坂入 祐一, 和田 啓伸, 鈴木 秀海, 岩田 剛和, 松下 一之, 千代 雅子, 吉野 一郎
  • Organizer: 日本呼吸器外科学会雑誌
• [Presentation] 胚培養液を用いたミトコンドリアDNAの非侵襲的解析 (2017)
  • Author(s): 曽根原 弘樹, 小林 達也, 石川 博士, 西村 基, 姚 躍, 西村 里美, 松下 一之, 大久保 毅, 瀬川 智也, 寺元 章吉, 田中 知明, 生水 真紀夫
  • Organizer: 日本卵子学会誌
• [Presentation] Establishment of screening network for hereditary cancers by genetic testing and counselling in Chiba Prefecture. (2017)
  • Author(s): Kazuyuki Matsushita.
  • Organizer: 日本癌学会
• [Presentation] Current condition and issues of clinical biobanks in Japan (2017)
  • Author(s): Kazuyuki Matsushita.
  • Organizer: Global Biobank Week, ISBER (Stockholm, Sweden)
  • Int'l Joint Research
• [Book] がんの臨床検査ハンドブック。がんと遺伝子検査「がん遺伝子パネル検査」 (2019)
  • Author(s): 松下一之
  • Total Pages: 150
  • Publisher: 日本医事新報社
• [Book] 「がん遺伝子パネル検査」検体検査の品質・精度確保の基準の手引き。 (2019)
  • Author(s): 松下一之
  • Total Pages: 200
  • Publisher: 医歯薬出版株式会社
• [Book] 検体検査の品質・精度確保の基準の手引き (2018)
  • Author(s): 宮地勇人、丸田秀夫、大西宏明、松下一之（分担執筆）ら。
  • Total Pages: 228
  • Publisher: 医歯薬出版株式会社
• [Book] がんの臨床検査ハンドブック (2018)
  • Author(s): 山田俊幸、前川真人、東田修二、松下一之（分担執筆）ら。
  • Total Pages: 256
  • Publisher: 日本医事新報社
• [Book] In silico 創薬におけるスクリーニングの高速化・高精度化技術 (2018)
  • Author(s): １．松下一之
  • Total Pages: 500
  • Publisher: （株）技術情報協会
• [Remarks]
  • URL: https://www.ho.chiba-u.ac.jp/section/kensa/index.html
• [Remarks] 千葉大学病院 検査部
  • URL: https://www.ho.chiba-u.ac.jp/section/kensa/index.html
• [Remarks] 千葉大学病院 遺伝子診療部
  • URL: https://www.ho.chiba-u.ac.jp/section/idenshi/index.html
• [Patent(Industrial Property Rights)] 芳香環含有化合物並びに癌の予防剤および／または治療剤 (2017)
  • Inventor(s): 松下一之、星野忠治他
  • Industrial Property Rights Holder: 国立大学法人千葉大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-110822
  • Filing Date: 2017
  • Acquisition Date: 2018