| Project/Area Number |
17K09028
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Teikyo University (2018-2021)
Aichi Cancer Center Research Institute (2017) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中西 速夫 愛知県がんセンター(研究所), 分子腫瘍学分野, 研究員 (20207830)
谷田部 恭 国立研究開発法人国立がん研究センター, 中央病院, 科長 (90280809)
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| Project Period (FY) |
2017-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | CTC / liquid biopsy / 肺癌 / CTC自動分離転写装置 / スライドグラス標本 / リキッドバイオプシー / 光学顕微鏡検出 / EGFR遺伝子変異解析 / 血液中循環腫瘍細胞 / 自動CTC分離・検出装置 / 細胞診 / 肺がん患者 / 肺静脈血 / Liquid Biopsy / フィルター型デバイス / Liquid biopsy / 血液循環がん細胞 |
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| Outline of Final Research Achievements |
For the separation of CTC, an automatic separation device using a metal filter developed by us was used. In this method, the cells were transferred onto a slide glass, immunostained (pan-cytokeratin antibody), and CTC was measured under an optical microscope. DNA was extracted from the identified CTC, and EGFR mutation was examined using digital PCR. Results: CTC was identified in 7 of 8 cases, of which EGFR mutations were detected in 3 cases. The results of the EGFR test from the resected tissue were consistent in 6 cases (2 cases with mutation and 4 cases without mutation), and 2 cases with disagreement were mutated in tissue / no mutation in CTC. One case was L858R in Ex19del / CTC in the tissue. We were able to confirm the applicability in clinical practice.
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| Academic Significance and Societal Importance of the Research Achievements |
本方法は簡易であるばかりでなく、効率の良いliquid biopsy としての応用の可能性も示された。院内での簡便なCTC検査＋liquid biopsy の可能性が広がった。
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