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The new method for detecting circulating tumor cells in lung cancerpatients

Research Project

Project/Area Number 17K09028
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory medicine
Research InstitutionTeikyo University (2018-2021)
Aichi Cancer Center Research Institute (2017)

Principal Investigator

sakao yukinori  帝京大学, 医学部, 教授 (00274605)

Co-Investigator(Kenkyū-buntansha) 中西 速夫  愛知県がんセンター(研究所), 分子腫瘍学分野, 研究員 (20207830)
谷田部 恭  国立研究開発法人国立がん研究センター, 中央病院, 科長 (90280809)
Project Period (FY) 2017-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsCTC / liquid biopsy / 肺癌 / CTC自動分離転写装置 / スライドグラス標本 / リキッドバイオプシー / 光学顕微鏡検出 / EGFR遺伝子変異解析 / 血液中循環腫瘍細胞 / 自動CTC分離・検出装置 / 細胞診 / 肺がん患者 / 肺静脈血 / Liquid Biopsy / フィルター型デバイス / Liquid biopsy / 血液循環がん細胞
Outline of Final Research Achievements

For the separation of CTC, an automatic separation device using a metal filter developed by us was used. In this method, the cells were transferred onto a slide glass, immunostained (pan-cytokeratin antibody), and CTC was measured under an optical microscope. DNA was extracted from the identified CTC, and EGFR mutation was examined using digital PCR. Results: CTC was identified in 7 of 8 cases, of which EGFR mutations were detected in 3 cases. The results of the EGFR test from the resected tissue were consistent in 6 cases (2 cases with mutation and 4 cases without mutation), and 2 cases with disagreement were mutated in tissue / no mutation in CTC. One case was L858R in Ex19del / CTC in the tissue. We were able to confirm the applicability in clinical practice.

Academic Significance and Societal Importance of the Research Achievements

本方法は簡易であるばかりでなく、効率の良いliquid biopsy としての応用の可能性も示された。院内での簡便なCTC検査+liquid biopsy の可能性が広がった。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2022 2021 2020 2019 2018 2017

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (6 results)

  • [Journal Article] CTCによるリキッドバイオプシーの現状と展望(総説)2022

    • Author(s)
      中西速夫、出嶋 仁、坂尾幸則、名取拓也、輪島健太郎、榊原康文、神田光朗
    • Journal Title

      泌尿器科

      Volume: 15 Pages: 22-28

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Dynamics of circulating tumor cells early after targeting therapy to human EGFR-mutated lung cancers and HER2 gene-amplified gastric cancers in mice.2019

    • Author(s)
      Ito A, Nakanishi H, Yoshimura M, Ito S, Sakao Y, Kodera Y, Yatabe Y, Kaneda N.
    • Journal Title

      Anticancer Res

      Volume: 39 Pages: 4811-4720

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Detection of abundant megakaryocytes in pulmonary artery blood in lung cancer patients using a microfluidic platform2018

    • Author(s)
      Dejima Hitoshi、Nakanishi Hayao、Kuroda Hiroaki、Yoshimura Mayumi、Sakakura Noriaki、Ueda Nanae、Ohta Yuko、Tanaka Rie、Mori Sayomi、Yoshida Tatsuya、Hida Toyoaki、Sawabata Noriyoshi、Yatabe Yasushi、Sakao Yukinori
    • Journal Title

      Lung Cancer

      Volume: 125 Pages: 128

    • DOI

      10.1016/j.lungcan.2018.09.011

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] 血中循環がん細胞(CTC)をスライドグラス上で光学顕微鏡により検出でき遺伝子解析も可能なCTC自動分離装置の開発2021

    • Author(s)
      中西速夫、坂尾幸則、出嶋 仁、名取拓也、輪島健太郎、榊原康文、神田光朗
    • Organizer
      第6回Liquid biopsy研究会
    • Related Report
      2021 Annual Research Report
  • [Presentation] フィルタ型自動CTC分離装置を用いたCTC細胞診の試みとその臨床応用2021

    • Author(s)
      中西速夫、坂尾幸則、出嶋 仁、名取拓也、輪島健太郎、榊原康文、神田光朗
    • Organizer
      第5回CTC臨床応用研究会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 血中循環がん細胞(CTC)を光学顕微鏡で検出できるCTC完全自動分離・染色システムの開発と臨床応用2020

    • Author(s)
      中西速夫、吉村真弓、筒山将之、岩田広治、坂尾幸則、松下和博
    • Organizer
      第4回Liquid Biopsy研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] Clinical study on CTC for GI tract , lung and breast cancer patients using a new cytology-based automated platform 「新規血中循環癌細胞(CTC) 自動細胞診標本作成装置の開発と消化器癌、肺癌, 乳癌CTCの臨床的検討」2019

    • Author(s)
      H. Nakanishi, H. Kuroda, H. Iwata, Y. Yatabe, S. Ito, Y. Sakao
    • Organizer
      第78回日本癌学会総会
    • Related Report
      2019 Research-status Report
  • [Presentation] 3Dフィルタ型CTC分離デバイスを用いたCTC細胞診の試みと大腸、肺, 乳がん血中循環癌細胞(CTC)に関する臨床的検討2018

    • Author(s)
      中西 速夫、筒山 将之、服部 正也、黒田 浩、谷田部 恭、伊藤 誠二、 坂尾 幸則、岩田広治
    • Organizer
      第77回日本癌学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] フィルター型分離デバイスを用いたCTC 細胞診断法の開発とその前臨床並びに臨床応用2017

    • Author(s)
      中西 速夫、伊藤 誠二、伊藤 友一、坂尾 幸則、黒田 浩、岩田広治、服部 正也、安立 弥生、筒山 将之、谷田部 恭
    • Organizer
      第76回日本癌学会学術総会.
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2023-01-30  

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