Changes in Enterovirus D68 Antibody Prevalence and Association with Epidemics

• Project/Area Number: 17K09080
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Epidemiology and preventive medicine
• Research Institution: Tohoku University
• Principal Investigator: Okamoto Michiko 東北大学, 医学系研究科, 助教 (10593981)
• Co-Investigator(Kenkyū-buntansha): Dapat Clyde 東北大学, 医学系研究科, 講師 (10733826) ; 斉藤 繭子 東北大学, 医学系研究科, 准教授 (20598031)
• Project Period (FY): 2017-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: エンテロウイルスD68型 / 血清抗体 / 血清抗体価 / ELISA抗体 / 中和抗体 / ELISA / 中和 / ウイルス / 感染症

Outline of Final Research Achievements

EV-D68 has been divided into four clades, A, B, C, and D. In the Philippines, outbreaks of Clade B have occurred every few years, and detections of Clade D have increased since 2017. Serum antibody titers were measured using Clade B and Clade D viruses isolated from children with acute respiratory infections from 2014 to 2017 as antigens. The antibody titer against Clade B, which frequently detected, was high throughout the study period, but Clade D antibody titers exceeded Clade B after the Clade D epidemic. However, regardless of the clade, the antibody titers decreased before the epidemic, suggesting that the decrease in antibody levels among children in the community may be related to the epidemic.

Academic Significance and Societal Importance of the Research Achievements

EV-D68の2つの遺伝子型のウイルスを用いて、地域における小児の抗体保有状況の推移を継続的に測定した研究である。2つのウイルス抗原は同じ血清型でありながら異なる推移を示したことから、遺伝子型間の抗原性が同一ではないことが示唆された。EV-D68は呼吸器疾患のみならずClade Bによる急性弛緩性麻痺との関連も重要視されているが、近年増加しているClade Dも注視する必要がある。

Research Products

• [Int'l Joint Research] Research Institute for Tropical Medicine(フィリピン)
• [Int'l Joint Research] Research Institute of Tropical Medicine(フィリピン)
• [Int'l Joint Research] Research Institute of Tropical Medicine(フィリピン)
• [Int'l Joint Research] Research Institute of Tropical Medicine(フィリピン)
• [Int'l Joint Research] Research Institute of Tropical Medicine(フィリピン)
• [Journal Article] Complete Genome Sequences of Enterovirus D68 Clade A and D Strains in the Philippines. (2021)
  • Author(s): Okamoto M, Sakamoto M, Dapat C, Saito M, Saito-Obata M, Tamaki R, Lupisan SP, Quiambao BP, Oshitani H.
  • Journal Title: Microbiol Resour Announc. Volume: 10(39) Issue: 39
  • DOI: 10.1128/mra.00709-21
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fluctuations in Antibody Titers against Enterovirus D68 in Pediatric Sera Collected in a Community before, during, and after a Possible Outbreak (2020)
  • Author(s): Kadji FMN, Nishimura H, Okamoto M, Sato K, Ohmiya S, Ito H, Suzuki A, Nagai Y, Oshitani H.
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 73 Issue: 1 Pages: 55-57
  • DOI: 10.7883/yoken.JJID.2019.056
  • NAID: 130007789150
  • ISSN: 1344-6304, 1884-2836
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Concurrent Community Transmission of Enterovirus D68 with Human Rhinoviruses and Respiratory Syncytial Virus among Children in Sendai, Japan. (2018)
  • Author(s): Metoki T, Okamoto M, Suzuki A, Kitaoka S, Miyabayashi H, Rokugo Y, Onuma R, Noguchi R, Sato T, Watanabe Y, Ohmiya S, Sato K, Nishimura H, Oshitani H, Kumaki S.
  • Journal Title: The Pediatric Infectious Disease Journal Volume: 37 Issue: 5 Pages: 394-400
  • DOI: 10.1097/inf.0000000000001768
  • Peer Reviewed