| Project/Area Number |
17K09165
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hygiene and public health
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
柳澤 裕之 東京慈恵会医科大学, 医学部, 教授 (10200536)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | ナノ粒子 / 酸化亜鉛 / 皮膚 / 分化 / 金属酸化物 / 産業保健 |
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| Outline of Final Research Achievements |
Metal oxide nanoparticles are widely used in cosmetics, but their exposure effects are also a concern. When zinc oxide nanoparticles were exposed to human skin keratinocyte HaCaT, the exosomes released by the cells were analyzed, and it was found that the particle size was smaller than usual, and that some keratins were included, suggesting that the cells were differentiated. We confirmed that the differentiation is PI3K/Akt pathway dependent with increase of involucrin. We also elucidated that zinc oxide nanoparticles induce cellular senescence. In addition, we found that the function of exosomes may differ among cells in their effect on cell migration.
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| Academic Significance and Societal Importance of the Research Achievements |
酸化亜鉛ナノ粒子に曝露された細胞が通常とは異なるエクソソームを放出していることが判明した。またエクソソームの解析を通じて、酸化亜鉛ナノ粒子に曝露された細胞が分化することも判明した。細胞の局所的な分化誘導は皮膚の恒常性、バリア機能に影響する可能性もあるが、塗布する場合は、既に分化の進行した最外層の細胞に浸透するだけなので悪影響は少ないと考えられ、むしろその中で浸透した細胞におけるインボルクリン産生増加により、皮膚バリア機能が向上する可能性も考えられる。
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