Grasp of actual situation and analyzing the resistance mechanism of ESBL-producing enterohemorrhagic Escherichia coli in Japan

• Project/Area Number: 17K09183
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hygiene and public health
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Ishihara Tomoko 国立感染症研究所, 研究企画調整センター, 室長 (30450555)
• Project Period (FY): 2017-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 薬剤耐性 / ESBL産生菌 / 腸管出血性大腸菌 / EHEC / 細菌

Outline of Final Research Achievements

In this study, we grasped the distribution of ESBL-producing enterohemorrhagic Escherichia coli EHEC and the actual condition of bacteriological characteristics on a nationwide scale. Previous studies have examined more than 470,000 healthy individuals and have shown that the incidence of EHEC carriers (398) is approximately 84.2 per 100,000.In this study, two ESBL-producing EHECs were confirmed among these healthy carrier-derived EHEC 398 strains, suggesting that the proportion of healthy carriers of CTX-resistant EHEC in Japan is about 0.5%.As a result of examining the bacteriological characteristics and ESBL type of the strain, it was EHEC O25: H28 vtx2a vtx2d strain belonging to the CTX-M8 group and EHEC O15: H16 vtx2g strain belonging to the CTX-M2 group.

Academic Significance and Societal Importance of the Research Achievements

本研究では、全国的な規模において健常者が保有する薬剤耐性下痢原性大腸菌、特にESBL産生 EHECの分布や特徴を評価することができた。これにより、国内における薬剤耐性下痢原性大腸菌の実態が明らかとなった。本研究成果は、健康保菌者由来EHECにおける国内におけるESBL産生EHECの拡散状況ならびにその細菌学的特徴の実態を明らかにするための科学的基盤の構築に寄与することが期待される。また、薬剤耐性の対策に取組む国内外の研究者ならびに関係機関に、今後の方針・あらゆる対策の基盤となる科学的データを提示し、日本における薬剤耐性の対策に貢献し得るものである。

Research Products

• [Journal Article] Large-scale genome analysis of bovine commensal Escherichia coli reveals that bovine-adapted E. coli lineages are serving as evolutionary sources of the emergence of human intestinal pathogenic strains. (2019)
  • Author(s): Arimizu Y, Kirino Y, Sato MP, Uno K, Sato T, Gotoh Y, Auvray F, Brugere H, Oswald E, Mainil JG, Anklam KS, Dopfer D, Yoshino S, Ooka T, Tanizawa Y, Nakamura Y, Iguchi A, Morita-Ishihara T, Ohnishi M, Akashi K, Hayashi T, Ogura Y
  • Journal Title: Genome Research Volume: 29 Issue: 9 Pages: 1495-1505
  • DOI: 10.1101/gr.249268.119
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Epidemiology and genotypic characterisation of dissemination patterns of uropathogenic Escherichia coli in a community (2019)
  • Author(s): Matsukawa M.、Igarashi M.、Watanabe H.、Qin L.、Ohnishi M.、Terajima J.、Iyoda S.、Morita-Ishihara T.、Tateda K.、Ishii Y.、Saga T.、Aoki K.、Bonomo R. A.
  • Journal Title: Epidemiology and Infection Volume: 147
  • DOI: 10.1017/s0950268819000426
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Geographically Widespread Outbreak Investigation and Development of a Rapid Screening Method Using Whole Genome Sequences of Enterohemorrhagic Escherichia coli O121 (2017)
  • Author(s): Lee Ken-ichi、Morita-Ishihara Tomoko、Iyoda Sunao、Ogura Yoshitoshi、Hayashi Tetsuya、Sekizuka Tsuyoshi、Kuroda Makoto、Ohnishi Makoto、EHEC Working Group in Japan
  • Journal Title: Frontiers in Microbiology Volume: 8 Pages: 701-701
  • DOI: 10.3389/fmicb.2017.00701
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of Shiga toxin-producing <i>Escherichia coli</i> from feces of sika deer (<i>Cervus nippon</i>) in Japan using PCR binary typing analysis to evaluate their potential human pathogenicity (2017)
  • Author(s): Kabeya H, Sato S, Oda S, Kawamura M, Nagasaka M, Kuranaga M, Yokoyama E, Hirai S, Iguchi A, Ishihara T, Kuroki T, Morita-Ishihara T, Iyoda S, Terajima J, Ohnishi M, Maruyama S.
  • Journal Title: Journal of Veterinary Medical Science Volume: 79 Issue: 5 Pages: 834-841
  • DOI: 10.1292/jvms.16-0568
  • NAID: 130005635466
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular epidemiological analyses using whole genome sequencing of enterohemorrhagic Escherichia coli (2017)
  • Author(s): Lee KI, Morita-Ishihara T, Izumiya H, Iyoda S, Ohnishi M
  • Journal Title: 化学療法の領域 Volume: 33 Pages: 137-141
• [Presentation] The increased incidence and epidemic strain of Shiga toxin-producing Escherichia coli O146 infection in Japan (2018)
  • Author(s): Tomoko Morita-Ishihara, Sunao Iyoda, Jun Terajima, Hidemasa Izumiya, Makoto Ohnishi, EHEC Working group
  • Organizer: 10th International Symposium on Shiga Toxin (Verocytotoxin) Producing Escherichia coli Infections agnosticians
  • Int'l Joint Research