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Mixture analysis and age estimation based on mitochondrial DNA polymophisms and methylation pattern

Research Project

Project/Area Number 17K09259
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Legal medicine
Research InstitutionAsahikawa Medical College

Principal Investigator

Masaru Asari  旭川医科大学, 医学部, 准教授 (40360979)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords個人識別 / 混合試料 / ミトコンドリアDNA / メチル化 / 法医学 / 混合DNA / 年齢推定 / ミトコンドリアDNA多型
Outline of Final Research Achievements

We developed a new method for discrimination of mitochondrial DNA haplotypes based on inhibition of amplification using designed probes including single nucleotide polymorphisms or reference sequences. Our method could effectively discriminate each haplotype from mitochondrial DNA mixtures. Although we did not find the useful methylation targets for age estimation of Japanese individuals, we confirmed that single bese extention method would be useful to detect lower methylation rates.

Academic Significance and Societal Importance of the Research Achievements

本研究結果は、きわめて微量な混合試料からのミトコンドリアDNA型解析法として法医実務へ利用可能である。特に検体数が多い場合においても、効率よく被疑者および被害者など含まれる個人のミトコンドリアDNA型が検出可能であると考える。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (3 results)

All 2019

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] Discrimination of haplotype in mitochondrial DNA mixtures using LNA-mediated PCR clamping.2019

    • Author(s)
      Asari M, Isozaki S, Hoshina C, Okuda K, Tanaka H, Horioka K, Shiono H, Shimizu K.
    • Journal Title

      Forensic Sci Int Genet.

      Volume: 41 Pages: 58-63

    • DOI

      10.1016/j.fsigen.2019.03.018

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] PCRクランピング法を用いた混合試料からのミトコンドリアDNA型解析2019

    • Author(s)
      浅利 優,磯崎翔太郎,保科千里,奥田勝博,田中宏樹,北村麻奈,島津雅子,堀岡希衣,塩野 寛,清水惠子
    • Organizer
      第103次日本法医学会学術全国集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 浅利 優,奥田勝博,保科千里,田中宏樹,堀岡希衣,塩野 寛,清水惠子2019

    • Author(s)
      配列特異的な増幅阻害に基づいた混合試料からのミトコンドリアDNA型解析
    • Organizer
      日本DNA多型学会第28回学術集会
    • Related Report
      2019 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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