Mixture analysis and age estimation based on mitochondrial DNA polymophisms and methylation pattern
Project/Area Number |
17K09259
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Legal medicine
|
Research Institution | Asahikawa Medical College |
Principal Investigator |
Masaru Asari 旭川医科大学, 医学部, 准教授 (40360979)
|
Project Period (FY) |
2017-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 個人識別 / 混合試料 / ミトコンドリアDNA / メチル化 / 法医学 / 混合DNA / 年齢推定 / ミトコンドリアDNA多型 |
Outline of Final Research Achievements |
We developed a new method for discrimination of mitochondrial DNA haplotypes based on inhibition of amplification using designed probes including single nucleotide polymorphisms or reference sequences. Our method could effectively discriminate each haplotype from mitochondrial DNA mixtures. Although we did not find the useful methylation targets for age estimation of Japanese individuals, we confirmed that single bese extention method would be useful to detect lower methylation rates.
|
Academic Significance and Societal Importance of the Research Achievements |
本研究結果は、きわめて微量な混合試料からのミトコンドリアDNA型解析法として法医実務へ利用可能である。特に検体数が多い場合においても、効率よく被疑者および被害者など含まれる個人のミトコンドリアDNA型が検出可能であると考える。
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Report
(4 results)
Research Products
(3 results)