Immunohistochemical analyses of candidate hypothermic markers in human forensic samples

• Project/Area Number: 17K09275
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Iwate Medical University
• Principal Investigator: Takamiya Masataka 岩手医科大学, 医学部, 講師 (30364334)
• Co-Investigator(Kenkyū-buntansha): 三枝 聖 岩手医科大学, 教養教育センター, 講師 (30398490)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 低体温症 / DNA microarray法 / 免疫組織化学 / 対応分析 / 法医解剖 / DNAマイクロアレイ

Outline of Final Research Achievements

We have analyzed the murine transcriptome of heart, lung, liver, adrenal gland, kidney killed by hypothermia using DNA microarray technology. These analyses indicated several candidate genes as forensic biomarkers of hypothermia. Expressions of these factors were investigated in human samples, with immunohistochemical methods. In addition, correspondence analysis was applied to murine DNA microarray datum of approximately 200000 genes.
Correspondence analysis demonstrated that both the increased and decreased genes affect the pathophysiology of the 5 organs. And, immunohistochemical expressions of lungs and adrenal glands would be useful in human forensic practices.

Academic Significance and Societal Importance of the Research Achievements

これまでの低体温症指標は非特異的なものが多い。分子生物学的が急速に進展している今日、新規手技の導入を積極的に進め、新規マーカーの検出を試みることが求められる。本研究は低体温症診断の進展に大きく貢献するものと考えられる。なお本研究は低体温症DNAマイクロアレイデータの実務応用への展開であるが、大規模遺伝子検索の適用は低体温症には限らない。DNAマイクロアレイを用い、低体温症以外の様々な病態における大容量遺伝子データを得ることは可能である。本研究により大規模遺伝子解析からの法医実務への応用の道が切り開かれれば、将来、他の病態における法医学的なバイオマーカー検索が急速に進展するものと考えられる。

Research Products

• [Journal Article] Molecular biological and statistical background of transcriptomic analyses for forensic pathophysiologic studies and biomarker detections (2018)
  • Author(s): Masataka Takamiya
  • Journal Title: Forensic Pathology Volume: 24 Pages: 33-52
  • Peer Reviewed
• [Journal Article] An Autopsy Case of Sudden Death in Neurofibromatosis Type 1 With Pheochromocytoma and Myocarditis (2018)
  • Author(s): Masataka Takamiya, Hisae Niitsu, Kiyoshi Saigusa
  • Journal Title: The American Journal of Forensic Medicine and Pathology Volume: 39 Pages: 78-81
  • Peer Reviewed
• [Presentation] マウス低体温症における肝臓のトランスクリプトーム解析 (2019)
  • Author(s): 高宮正隆，三枝 聖，出羽厚二
  • Organizer: 第103次日本法医学会学術全国集会
• [Presentation] Incorporation of the gene expression omnibus into forensic pathology: reanalysis and recycling of large-scale gene expression data (2019)
  • Author(s): Masataka Takamiya, Kiyoshi Saigusa, Koji Dewa
  • Organizer: The 2nd Wakayama Medical University International Symposium of Forensic Molecular Pathology
  • Int'l Joint Research / Invited
• [Presentation] Pathophysiological and transcriptomic analysis of hypothermic mouse pulmonary tissue using DNA microarray (2018)
  • Author(s): Masataka Takamiya, Kiyoshi Saigusa, Koji Dewa
  • Organizer: 24th Congress of the International Academy of Legal Medicine
  • Int'l Joint Research
• [Presentation] Large-scale gene expression analysis in hypothermia to study pathophysiology and identify forensic biomarkers: Incorporating correspondence analysis into forensic pathology (2017)
  • Author(s): Masataka Takamiya
  • Organizer: The 1st Wakayama Medical University International Symposium of Forensic Molecular Pathology
  • Int'l Joint Research