| Project/Area Number |
17K09281
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | メチル化解析 / 年齢推定 / 心臓突然死 / DNAメチル化解析 / 心筋 / ストレスマーカー / ストレス定量 / ＤNAメチル化解析 / 体液同定 |
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| Outline of Final Research Achievements |
The association of the ATP10A gene was suggested by genome-wide methylation analysis in the case of sudden infant death. Furthermore, methylation analysis of 16 CpG sites of 8 genes (ELOVL2, CCDC10, C1orf1, FHL2, ASPA, F5, KLF14, PDE4C) could estimate the age of the donor within ± 6 years. Finally, the "Methylation Panel" which could analyze these at once was established.
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| Academic Significance and Societal Importance of the Research Achievements |
死因の判断が困難な乳幼児の突然死例において，メチル化解析により新たなバイオマーカーとなり得るATP10A遺伝子の関連が示唆され，今後の研究が期待される．また，現場に残された生体試料から由来者の年齢推定が±６歳の精度で可能となり，犯罪捜査において非常に有用な方法が確立された．このように本研究の成果は，即座に法医実務に応用可能なものであり，メチル化解析の応用は今後さらなる発展が期待される．
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