Proteomic analysis of the mode of action of antidepressive-like effect of a Kampo (traditional Japanese) medicine "kososan" extract.
| Project/Area Number |
17K09319
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General internal medicine(including psychosomatic medicine)
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| Research Institution | Kitasato University |
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Principal Investigator |
NAGAI Takayuki 北里大学, 大学院感染制御科学府, 准教授 (00172487)
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| Co-Investigator(Kenkyū-buntansha) |
伊藤 直樹 北里大学, 東洋医学総合研究所, 上級研究員 (00370164)
小寺 義男 北里大学, 理学部, 教授 (60265733)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 漢方薬 / 香蘇散 / うつ / 社会的ストレス / マウスモデル / 海馬 / プロテオーム解析 / 安定同位体標識法 / 脳・神経 / プロテオーム / 薬学 / 薬理学 |
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| Outline of Final Research Achievements |
We examined whether “kososan”, a Kampo (traditional Japanese herbal) medicine, affected social behavior in mice exposed to chronic social defeat stress (CSDS), an animal model of psychosocial stress, and then, proteomic analysis with stable isotope labeling was used to identify brain hippocampus proteins that are affected by kososan treatment.
As a result, oral administration of kososan extract alleviated social avoidance, a depression-like behavior, caused by CSDS exposure, and it was revealed that the animal feed was involved in its activity. The expression of 185 proteins was increased or decreased in the hippocampus of the model mice, but 24 proteins, including psychiatric disorder- and inflammation-related proteins, recovered on kososan treatment.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、社会的敗北ストレス(CSDS)誘発うつ様モデルマウスで漢方方剤「香蘇散」の抗うつ様活性が評価できることが明らかとなったことから、対人ストレスで惹起されるうつ症状に有効な漢方薬などの薬剤の評価に本モデルが有用であることが示された。
また、網羅的にタンパク質を解析できるプロテオーム解析により、うつ様症状に伴って脳（海馬）で発現量が変化し、香蘇散によるうつ様症状の改善によって発現量が正常状態に回復するタンパク質が同定されたことから、同解析法がうつ症状の発症機序や抗うつ薬の薬効機序の解明に有用であることが示唆された。
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Stimulation of entorhinal cortex-dentate gyrus circuitry is antidepressive.2018
Author(s)
Yun S, Reynolds RP, Petrof I, White A, Rivera PD, Segev A, Gibson AD, Suarez M, DeSalle MJ, Ito N, Mukherjee S, Richardson DR, Kang CE, Ahrens-Nicklas RC, Soler I, Chetkovich DM, Kourrich S, Coulter DA, Eisch AJ
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Journal Title
Nature Medicine
Volume: 24
Issue: 5
Pages: 658-666
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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