Antigen epitopes of serum anti-Helicobacter pylori antibody along H. pylori infection to gastric cancer onset
| Project/Area Number |
17K09353
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Oita University |
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Principal Investigator |
Akada Junko 大分大学, 医学部, 助教 (30346548)
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| Co-Investigator(Kenkyū-buntansha) |
村上 和成 大分大学, 医学部, 教授 (00239485)
山岡 吉生 大分大学, 医学部, 教授 (00544248)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ヘリコバクター・ピロリ / 血清 / 抗体 / 胃癌 / アジア / 既感染 / 血清抗体 / CagA / 抗原エピトープ / ペプチド / エピトープ |
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| Outline of Final Research Achievements |
Helicobacter pylori induce gastric cancer after life-long chronic infection. To promote gastric cancer prevention, it is necessary to find gastric cancer patients in very early stage, or high-risk persons of gastric cancer before onset, at the occasion of population-based mass-screening of gastric cancer. Using non-invasive serum samples, we evaluated regional characters and disease-related characters of anti-H. pylori and anti-CagA antibodies. We identified two of major antigenic epitopes of anti-CagA antibody using comprehensive CagA antigen epitope peptide ELISA array. We could not analyze transition of antigen epitopes variations along the process of gastric cancer onset.
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| Academic Significance and Societal Importance of the Research Achievements |
ピロリ菌抗体情報量を飛躍的に広げ、ピロリ菌感染症抗体変遷の全体像が掌握し、ピロリ菌抗体検査で問題となっている陰性高値検体の科学的実態を明確にできれば、ピロリ菌抗体検査の改良点を見出せるのではないかと考えるが、そこまで到達することができなかった。学術的には抗体の背景にあるエピトープ特異的免疫反応の研究へと進む必要がある。血清抗体を手掛かりに、ピロリ菌慢性感染症においていかに炎症発癌が誘導されるのか、研究の進展を期待する。
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Report
(5 results)
Research Products
(24 results)
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[Journal Article] Serum Helicobacter pylori antibody reactivity in seven Asian countries using an automated latex aggregation turbidity assay2021
Author(s)
Akada J,Tshibangu‐K,Tuan V,Kurogi S,Matsuo Y,Ansari S,Doohan D,Phuc B,Subsomwong P,Waskito L,Binh T,Nguyen L,Khien V,Dung H,Miftahussurur M,Syam A,Tshering L,Vilaichone R,Mahachai V,Ratanachu‐ek T,Shrestha P,Yee T,Htet K,Aftab H,Matsuhisa T,Uchida T,Okimoto T,Mizukami K,Kodama M,Murakami K,Takahashi N,Yamaoka Yoshio
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Journal Title
Journal of Gastroenterology and Hepatology
Volume: -
Issue: 8
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Characterization of a novel Helicobacter pylori East Asian-type CagA ELISA for detecting patients infected with various cagA genotypes2020
Author(s)
Doohan D, Miftahussurur M, Matsuo Y, Kido Y, Akada J, Matsuhisa T, Yee TT, Htet K, Aftab H, Vilaichone RK, Mahachai V, Ratanachu-Ek T, Tshering L, Waskito LA, Fauzia KA, Uchida T, Syam AF, Rezkitha YAA, Yamaoka Y
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Journal Title
Medcal Microbiology and Immunology
Volume: 209
Issue: 1
Pages: 29-40
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Novel CagA ELISA exhibits enhanced sensitivity of Helicobacter pylori CagA antibody.2017
Author(s)
Matsuo Y, Kido Y, Akada J, Shiota S, Binh TT, Trang TT, Dung HD, Tung PH, Tri TD, Thuan NP, Tam LQ, Nam BC, Khien VV, Yamaoka Y.
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Journal Title
World J Gastroenterol
Volume: 23(1)
Issue: 1
Pages: 48-59
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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