| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In this study, the morphological analysis for the enteric nervous system (ENS) was performed with a new electron microscopic analysis, a focused ion beam / scanning electron microscopy (FIB/SEM). With the FIB/SEM, we can obtain big volume and three-dimensional ultrastructural images. Especially, we focused on complicated structures of synaptic structures and their interactions with enteric glial cells surrounding them. This strategy made it possible to accelerate the understanding of basic morphological findings in order to explain a complicated system for controlling gut functions. Furthermore, high-pressure cryo fixations were performed in order to keep more native tissue structures, comparing with the normal chemical fixations. It could be a critical point how the native structures could be maintained during quantitative analyses with FIB/SEM data.
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