| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We performed transport experiment of PGE2 using the oocyte which is expressing mutant SLCO2A1 gene (940+1GA, 664GA, 1807CT, 421GT, 1372GT, 547GA, 1647GT, 97GC, 770GA, 830dupT, 830delT) which have been identified in CEAS patients. In 9 mutations (940+1GA, 664GA, 1807CT, 421GT, 547GA, 1647GT, 770GA, 830dupT, 830delT), PGE2 transport was lost completely. In 1372GT, PGE2 transport remained partially lost. In 97GC, PGE2 transport was maintained at the same level of wild-type. It was also revealed that the mutant SLCO2A1 protein had a difference in the Vmax value, which is the uptake amount per hour, rather than the Km value indicating affinity.
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