Functional analysis of SLCO2A1 transporter
| Project/Area Number |
17K09394
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
久松 理一 杏林大学, 医学部, 教授 (60255437)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 小腸潰瘍 / SLCO2A1遺伝子 / プロスタグランジン / トランスポーター / SLCO2A1 / CEAS |
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| Outline of Final Research Achievements |
We performed transport experiment of PGE2 using the oocyte which is expressing mutant SLCO2A1 gene (940+1GA, 664GA, 1807CT, 421GT, 1372GT, 547GA, 1647GT, 97GC, 770GA, 830dupT, 830delT) which have been identified in CEAS patients. In 9 mutations (940+1GA, 664GA, 1807CT, 421GT, 547GA, 1647GT, 770GA, 830dupT, 830delT), PGE2 transport was lost completely. In 1372GT, PGE2 transport remained partially lost. In 97GC, PGE2 transport was maintained at the same level of wild-type. It was also revealed that the mutant SLCO2A1 protein had a difference in the Vmax value, which is the uptake amount per hour, rather than the Km value indicating affinity.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究においてCEAS症例で報告されているSLCO2A1遺伝子の変異による各変異型の特性が明らかとなったことにより、重症度を含めた臨床像や尿中プロスタグランジンE代謝物値との照合が可能となった。今後、厚生労働省難治性疾患研究事業難治性炎症性腸疾患に関する調査研究により現在作成されているCEASデータベースと本研究結果の照合により、遺伝子情報と臨床像が結びつくことが期待される。
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Report
(4 results)
Research Products
(3 results)
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[Journal Article] Measurement of prostaglandin metabolites is useful in diagnosis of small bowel ulcerations.2019
Author(s)
Matsuno Y, Umeno J, Esaki M, Hirakawa Y, Fuyuno Y, Okamoto Y, Hirano A, Yasukawa S, Hirai F, Matsui T, Hosomi S, Watanabe K, Hosoe N, Ogata H, Hisamatsu T, Yanai S, Kochi S, Kurahara K, Yao T, Torisu T, Kitazono T, Matsumoto T.
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Journal Title
World J Gastroenterol
Volume: Apr 14;25(14):
Issue: 14
Pages: 1753-1763
DOI
Related Report
Peer Reviewed
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