肝臓特異的ハイドロダイナミック法による核酸医薬送達と肝癌遺伝子治療法への応用

• Project/Area Number: 17K09408
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Niigata University
• Principal Investigator: 上村 顕也 新潟大学, 医歯学総合病院, 講師 (00579146)
• Co-Investigator(Kenkyū-buntansha): 寺井 崇二 新潟大学, 医歯学系, 教授 (00332809) ; 坂牧 僚 新潟大学, 医歯学系, 助教 (40792289) ; 横尾 健 新潟大学, 医歯学総合研究科, 特任助教 (80750629)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Discontinued (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 遺伝子治療 / ハイドロダイナミック遺伝子導入法 / 肝癌 / 核酸医薬 / ジフテリアトキシン / 肝癌遺伝子治療 / 肝癌遺伝子治療法 / 肝臓特異的遺伝子導入

Outline of Annual Research Achievements

本研究は、ハイドロダイナミック遺伝子導入法(HGD)を肝癌治療に応用するための方法論、抗腫瘍効果を学術的に検証するためのステップと位置づけ、HGDパラメーターの確立、治療遺伝子の選択、効果と安全性の検証、肝癌モデル動物に対する治療効果の検証を行うことを目的とした。

今年度は前二年度に肝癌の増殖制御に有用であることを明らかにした、ジフテリアトキシンA遺伝子を用いた肝癌に対する遺伝子治療確立のためのマウス肝癌モデルに対する遺伝子治療効果を検証した。
これまでに、in vitroでは、ジフテリアトキシンA発現遺伝子が肝細胞癌に対して抗腫瘍効果を有することを明らかにし、同遺伝子を肝癌で発現するAFPのプロモーター制御下に発現するコンストラクトを作成した。今年度は、発がん遺伝子をハイドロダイナミック遺伝子導入法でマウス肝に遺伝子導入して作製したマウス肝癌モデルに対する、ジフテリアトキシン遺伝子Aの遺伝子治療効果を検討した。また、プロモーター選択性による遺伝子治療の安全性も検証した。
具体的には、肝癌マウスモデルに対して、腫瘍発生の各段階において、ジフテリアトキシンA遺伝子発現コンストラクトによる遺伝子治療を行い、腫瘍数、腫瘍径、腫瘍マーカー、などにより治療効果を、血液生化学的評価による安全性の検討を行った。
その結果、肝癌モデルマウスにおいても肝発癌率の抑制、腫瘍マーカーの低下を認め、ジフテリアトキシンA遺伝子による肝癌遺伝子治療の効果を実証することができた。またプロモーター選択性により、肝障害の観点からも安全な遺伝子治療が可能であった。本研究の成果により、プロモータ選択性によりジフテリアトキシンA遺伝子を用いた種々の悪性疾患治療が可能であることが示唆された。

Research Products

• [Journal Article] Ghrelin-insulin-like growth factor-1 axis is activated via autonomic neural circuits in the non-alcoholic fatty liver disease. (2020)
  • Author(s): Nagoya T, Kamimura K, Inoue R, Ko M, Owaki T, Niwa Y, Sakai N, Setsu T, Sakamaki A, Yokoo T, Kamimura H, Nakamura Y, Ueno M, Terai S.
  • Journal Title: Neurogastroenterol Motil Volume: 32 Issue: 5
  • DOI: 10.1111/nmo.13799
  • Peer Reviewed / Open Access
• [Journal Article] Prostaglandin E2 and its receptor EP2 trigger signaling that contributes to YAP-mediated cell competition. (2020)
  • Author(s): Ishihara E, Nagaoka Y, Okuno T, Kofuji S, Ishigami-Yuasa M, Kagechika H, Kamimura K, Terai S, Yokomizo T, Sugimoto Y, Fujita Y, Suzuki A, Nishina H.
  • Journal Title: Genes Cells. Volume: 25(3) Issue: 3 Pages: 197-214
  • DOI: 10.1111/gtc.12750
  • NAID: 120006873037
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rare Brain Metastasis From a Pancreatobiliary Subtype of Intraductal Papillary Mucinous Neoplasm (2020)
  • Author(s): Arao Yoshihisa、Kamimura Kenya、Ikemi Masatoshi、Hayashi Kazunao、Takaki Masayuki、Takahashi Shunsaku、Seino Satoshi、Abe Hiroyuki、Tsuboguchi Shintaro、Otsu Yutaka、Sanpei Kazuhiro、Kohisa Junji、Kondo Shuhei、Tani Yusuke、Ito Junko、Toyoshima Yasuko、Kakita Akiyoshi、Ajioka Yoichi、Terai Shuji
  • Journal Title: Pancreas Volume: 49 Issue: 1 Pages: e8-e11
  • DOI: 10.1097/mpa.0000000000001450
  • Peer Reviewed / Open Access
• [Journal Article] Effect of Diphtheria Toxin-Based Gene Therapy for Hepatocellular Carcinoma (2020)
  • Author(s): Kamimura Kenya、Yokoo Takeshi、Abe Hiroyuki、Sakai Norihiro、Nagoya Takuro、Kobayashi Yuji、Ohtsuka Masato、Miura Hiromi、Sakamaki Akira、Kamimura Hiroteru、Miyamura Norio、Nishina Hiroshi、Terai Shuji
  • Journal Title: Cancers Volume: 12 Issue: 2 Pages: 472-472
  • DOI: 10.3390/cancers12020472
  • Peer Reviewed / Open Access
• [Journal Article] Huge liver tumor in young man (2020)
  • Author(s): Shibata Osamu、Kamimura Kenya、Azumi Rie、Shibuya Kei、Shiba Shintaro、Ohno Tatsuya、Terai Shuji
  • Journal Title: JGH Open Volume: 4 Issue: 2 Pages: 301-302
  • DOI: 10.1002/jgh3.12234
  • Peer Reviewed
• [Journal Article] Rare Mesenteric Arterial Diseases: Fibromuscular Dysplasia and Segmental Arterial Mediolysis and Literature Review (2019)
  • Author(s): Ko Masayoshi、Kamimura Kenya、Sakamaki Akira、Niwa Yusuke、Tominaga Kentaro、Mizuno Kenichi、Terai Shuji
  • Journal Title: Internal Medicine Volume: 58 Issue: 23 Pages: 3393-3400
  • DOI: 10.2169/internalmedicine.3094-19
  • NAID: 130007754642
  • ISSN: 0918-2918, 1349-7235
  • Year and Date: 2019-12-01
  • Peer Reviewed
• [Journal Article] A Rare Duodenal Carcinosarcoma: A Case Report and Literature Review (2019)
  • Author(s): Arao Yoshihisa、Kamimura Kenya、Ikemi Masatoshi、Takaki Masayuki、Takahashi Shunsaku、Seino Satoshi、Abe Hiroyuki、Kohisa Junji、Kato Takashi、Ajioka Yoichi、Terai Shuji
  • Journal Title: Internal Medicine Volume: 58 Issue: 9 Pages: 1273-1278
  • DOI: 10.2169/internalmedicine.2094-18
  • NAID: 130007641496
  • ISSN: 0918-2918, 1349-7235
  • Year and Date: 2019-05-01
  • Peer Reviewed
• [Journal Article] Usefulness of Capsule Endoscopy and Double-balloon Enteroscopy for the Diagnosis of Multiple Carcinoid Tumors in the Small Intestine: Case Reports and a Literature Review (2019)
  • Author(s): Tominaga Kentaro、Kamimura Kenya、Yokoyama Junji、Terai Shuji
  • Journal Title: Internal Medicine Volume: 58 Issue: 5 Pages: 655-659
  • DOI: 10.2169/internalmedicine.1700-18
  • NAID: 130007606549
  • ISSN: 0918-2918, 1349-7235
  • Year and Date: 2019-03-01
  • Peer Reviewed
• [Journal Article] Esophageal Stenosis by Prostate Cancer Metastasis (2019)
  • Author(s): Kamimura Kenya、Shinagawa-Kobayashi Yoko、Mizuno Kenichi、Terai Shuji
  • Journal Title: The American Journal of Gastroenterology Volume: 114 Issue: 4 Pages: 685-687
  • DOI: 10.14309/ajg.0000000000000185
  • Peer Reviewed
• [Journal Article] Identification of molecular transition of hepatocellular carcinoma: a novel method to predict the initiation of metastasis (2019)
  • Author(s): Kamimura Kenya
  • Journal Title: Stem Cell Investigation Volume: 6 Pages: 5-5
  • DOI: 10.21037/sci.2019.02.02
  • Peer Reviewed
• [Journal Article] Inhibition of sodium glucose cotransporter 2 (SGLT2) delays liver fibrosis in a medaka model of nonalcoholic steatohepatitis (NASH) (2019)
  • Author(s): Goto Ryo、Kamimura Kenya、Shinagawa‐Kobayashi Yoko、Sakai Norihiro、Nagoya Takuro、Niwa Yusuke、Ko Masayoshi、Ogawa Kohei、Inoue Ryosuke、Yokoo Takeshi、Sakamaki Akira、Kamimura Hiroteru、Abe Satoshi、Nishina Hiroshi、Terai Shuji
  • Journal Title: FEBS Open Bio Volume: 9 Issue: 4 Pages: 643-652
  • DOI: 10.1002/2211-5463.12598
  • Peer Reviewed / Open Access
• [Journal Article] Considerations of elderly factors to manage the complication of liver cirrhosis in elderly patients (2019)
  • Author(s): Kamimura Kenya、Sakamaki Akira、Kamimura Hiroteru、Setsu Toru、Yokoo Takeshi、Takamura Masaaki、Terai Shuji
  • Journal Title: World Journal of Gastroenterology Volume: 25 Issue: 15 Pages: 1817-1827
  • DOI: 10.3748/wjg.v25.i15.1817
  • Peer Reviewed
• [Journal Article] Efficacy and safety of ribavirin therapy for chronic hepatitis E after kidney transplantation (2019)
  • Author(s): Yoshida Tomoaki、Takamura Masaaki、Goto Ryo、Takeuchi Suguru、Tsuchiya Atsunori、Kamimura Kenya、Tasaki Masayuki、Nakagawa Yuki、Saito Kazuhide、Tomita Yoshihiko、Terai Shuji
  • Journal Title: Hepatology Research Volume: 49 Issue: 10 Pages: 1244-1248
  • DOI: 10.1111/hepr.13363
  • Peer Reviewed
• [Journal Article] Multiple Endocrine Neoplasia Type 1?Related Acute Pancreatitis (2019)
  • Author(s): Takahashi Shunsaku、Kamimura Kenya、Abe Hiroyuki、Watanabe Yukari、Hayashi Kazunao、Mizuno Kenichi、Yamamoto Takashi、Takeuchi Manabu、Natsui Masaaki、Terai Shuji
  • Journal Title: Pancreas Volume: 48 Issue: 5 Pages: e35-e38
  • DOI: 10.1097/mpa.0000000000001267
  • Peer Reviewed
• [Journal Article] Rational arrangement of measuring shear wave speed in the liver (2019)
  • Author(s): Yokoo Takeshi、Kanefuji Tsutomu、Suda Takeshi、Nagayama Itsuo、Hoshi Takahiro、Abe Satoshi、Morita Shinichi、Kamimura Hiroteru、Kamimura Kenya、Tsuchiya Atsunori、Takamura Masaaki、Yagi Kazuyoshi、Terai Shuji
  • Journal Title: World Journal of Gastroenterology Volume: 25 Issue: 20 Pages: 2503-2513
  • DOI: 10.3748/wjg.v25.i20.2503
  • Peer Reviewed
• [Journal Article] <p>Effective prevention of sorafenib-related vascular damage induced adverse events and maintenance of hepatic function by dried bonito broth and histidine</p> (2019)
  • Author(s): Sakai Norihiro、Kamimura Kenya、Shinagawa-Kobayashi Yoko、Nagoya Takuro、Niwa Yusuke、Ko Masayoshi、Setsu Toru、Sakamaki Akira、Yokoo Takeshi、Abe Satoshi、Kamimura Hiroteru、Sugitani Soichi、Yanagi Masahiko、Terai Shuji
  • Journal Title: Cancer Management and Research Volume: Volume 11 Pages: 4437-4448
  • DOI: 10.2147/cmar.s201424
  • Peer Reviewed
• [Journal Article] Efficacy of gelatin hydrogels incorporating triamcinolone acetonide for (2019)
  • Author(s): Nao Nakajima, Satoru Hashimoto, Hiroki Sato, Kazuya Takahashi, Takuro Nagoya, Kenya Kamimura, Atsunori Tsuchiya, Junji Yokoyama, Yuichi Sato, Hanako Wakatsuki, Masayuki Miyata, Yusuke Akashi, Ryusuke Tanaka, Ken Matsuda, Yasuhiko Tabata, Shuji Terai
  • Journal Title: Regenerative Therapy Volume: 11 Pages: 41-46
  • DOI: 10.1016/j.reth.2019.04.001
  • Peer Reviewed / Open Access
• [Journal Article] A case report of psychiatric symptoms following direct-acting antiviral and ribavirin combination therapy for chronic hepatitis C in a patient with innate anxiety (2019)
  • Author(s): Sakamaki Akira、Kamimura Kenya、Fukui Naoki、Watanabe Haruka、Sakai Norihiro、Tominaga Kentaro、Mizuno Kenichi、Takamura Masaaki、Kawai Hirokazu、Sugai Takuro、Yamagiwa Satoshi、Someya Toshiyuki、Terai Shuji
  • Journal Title: BMC Gastroenterology Volume: 19 Issue: 1 Pages: 85-85
  • DOI: 10.1186/s12876-019-1013-1
  • Peer Reviewed
• [Journal Article] Colorectal neuroendocrine carcinoma: A case report and review of the literature (2019)
  • Author(s): Yoshida Tomoaki、Kamimura Kenya、Hosaka Kazunori、Doumori Koji、Oka Hiromitsu、Sato Akito、Fukuhara Yasuo、Watanabe Shoji、Sato Tomomi、Yoshikawa Akira、Tomidokoro Takashi、Terai Shuji
  • Journal Title: World Journal of Clinical Cases Volume: 7 Issue: 14 Pages: 1865-1875
  • DOI: 10.12998/wjcc.v7.i14.1865
  • Peer Reviewed
• [Journal Article] Change in body composition in patients with achalasia before and after peroral endoscopic myotomy (2019)
  • Author(s): Mizusawa Takeshi、Sato Hiroki、Kamimura Kenya、Hashimoto Satoru、Mizuno Ken‐ichi、Kamimura Hiroteru、Ikarashi Satoshi、Hayashi Kazunao、Takamura Masaaki、Yokoyama Junji、Terai Shuji
  • Journal Title: Journal of Gastroenterology and Hepatology Volume: 35 Issue: 4 Pages: 601-608
  • DOI: 10.1111/jgh.14847
  • Peer Reviewed
• [Journal Article] Inhibition of sodium‐glucose cotransporter 2 ameliorates renal injury in a novel medaka model of nonalcoholic steatohepatitis‐related kidney disease (2019)
  • Author(s): Nagoya Takuro、Kamimura Kenya、Goto Ryo、Shinagawa‐Kobayashi Yoko、Niwa Yusuke、Kimura Atsushi、Sakai Norihiro、Ko Masayoshi、Nishina Hiroshi、Terai Shuji
  • Journal Title: FEBS Open Bio Volume: 9 Issue: 12 Pages: 2016-2024
  • DOI: 10.1002/2211-5463.12734
  • Peer Reviewed
• [Journal Article] Methotrexate-related lymphoproliferative disorders in the liver: Case presentation and mini-review (2019)
  • Author(s): Mizusawa Takeshi、Kamimura Kenya、Sato Hiroki、Suda Takeshi、Fukunari Hiroyuki、Hasegawa Go、Shibata Osamu、Morita Shinichi、Sakamaki Akira、Yokoyama Junji、Saito Yu、Hori Yoshihisa、Maruyama Yuduru、Yoshimine Fumitoshi、Hoshi Takahiro、Morita Shinichi、Kanefuji Tsutomu、Kobayashi Masaaki、Terai Shuji
  • Journal Title: World Journal of Clinical Cases Volume: 7 Issue: 21 Pages: 3553-3561
  • DOI: 10.12998/wjcc.v7.i21.3553
  • Peer Reviewed
• [Journal Article] Gene Therapy for Liver Cancers: Current Status from Basic to Clinics (2019)
  • Author(s): Kamimura Kenya、Yokoo Takeshi、Abe Hiroyuki、Terai Shuji
  • Journal Title: Cancers Volume: 11 Issue: 12 Pages: 1865-1865
  • DOI: 10.3390/cancers11121865
  • Peer Reviewed
• [Journal Article] Pulmonary tumor thrombotic microangiopathy of hepatocellular carcinoma: A case report and review of literature (2019)
  • Author(s): Morita Shinichi、Kamimura Kenya、Abe Hiroyuki、Watanabe-Mori Yukari、Oda Chiyumi、Kobayashi Takamasa、Arao Yoshihisa、Tani Yusuke、Ohashi Riuko、Ajioka Yoichi、Terai Shuji
  • Journal Title: World Journal of Gastroenterology Volume: 25 Issue: 48 Pages: 6949-6958
  • DOI: 10.3748/wjg.v25.i48.6949
  • Peer Reviewed
• [Journal Article] Antiprogrammed Cell Death-1 Immunotherapy-Related Secondary Sclerosing Cholangitis (2018)
  • Author(s): Ogawa Kohei、Kamimura Kenya、Terai Shuji
  • Journal Title: Hepatology Volume: 69 Issue: 2 Pages: 914-916
  • DOI: 10.1002/hep.30189
  • Peer Reviewed
• [Journal Article] Effect of histidine on sorafenib-induced vascular damage: Analysis using novel medaka fish model. (2018)
  • Author(s): Shinagawa-Kobayashi Y, Kamimura K, Goto R, Ogawa K, Inoue R, Yokoo T, Sakai N, Nagoya T, Sakamaki A, Abe S, Sugitani S, Yanagi M, Fujisawa K, Nozawa Y, Koyama N, Nishina H, Furutani- Seiki M, Sakaida I, Terai S.
  • Journal Title: Biochem Biophys Res Commun. Volume: 496 Issue: 2 Pages: 556-561
  • DOI: 10.1016/j.bbrc.2018.01.057
  • Peer Reviewed
• [Journal Article] Effect of a neural relay on liver regeneration in mice: activation of serotonin release from the gastrointestinal tract (2018)
  • Author(s): Inoue Ryosuke、Kamimura Kenya、Nagoya Takuro、Sakai Norihiro、Yokoo Takeshi、Goto Ryo、Ogawa Kohei、Shinagawa-Kobayashi Yoko、Watanabe-Mori Yukari、Sakamaki Akira、Abe Satoshi、Kamimura Hiroteru、Miyamura Norio、Nishina Hiroshi、Terai Shuji
  • Journal Title: FEBS Open Bio Volume: 8 Issue: 3 Pages: 449-460
  • DOI: 10.1002/2211-5463.12382
  • Peer Reviewed / Open Access
• [Journal Article] A case of Beh?et’s disease with various gastrointestinal findings (2018)
  • Author(s): Tominaga Kentaro、Kamimura Kenya、Takahashi Kazuya、Yokoyama Junji、Terai Shuji
  • Journal Title: Clinical Journal of Gastroenterology Volume: 11 Issue: 5 Pages: 354-358
  • DOI: 10.1007/s12328-018-0860-6
  • Peer Reviewed
• [Journal Article] Autonomic nervous system network and liver regeneration (2018)
  • Author(s): Kamimura Kenya、Inoue Ryosuke、Nagoya Takuro、Sakai Norihiro、Goto Ryo、Ko Masayoshi、Niwa Yusuke、Terai Shuji
  • Journal Title: World Journal of Gastroenterology Volume: 24 Issue: 15 Pages: 1616-1621
  • DOI: 10.3748/wjg.v24.i15.1616
  • Peer Reviewed
• [Journal Article] Diversion colitis and pouchitis: A mini-review (2018)
  • Author(s): Tominaga Kentaro、Kamimura Kenya、Takahashi Kazuya、Yokoyama Junji、Yamagiwa Satoshi、Terai Shuji
  • Journal Title: World Journal of Gastroenterology Volume: 24 Issue: 16 Pages: 1734-1747
  • DOI: 10.3748/wjg.v24.i16.1734
  • Peer Reviewed
• [Journal Article] Effective prevention of sorafenib-induced hand&ndash;foot syndrome by dried-bonito broth (2018)
  • Author(s): Kamimura Kenya、Shinagawa-Kobayashi Yoko、Goto Ryo、Ogawa Kohei、Yokoo Takeshi、Sakamaki Akira、Abe Satoshi、Kamimura Hiroteru、Suda Takeshi、Baba Hiroshi、Tanaka Takayuki、Nozawa Yoshizu、Koyama Naoto、Takamura Masaaki、Kawai Hirokazu、Yamagiwa Satoshi、Aoyagi Yutaka、Terai Shuji
  • Journal Title: Cancer Management and Research Volume: Volume 10 Pages: 805-813
  • DOI: 10.2147/cmar.s159370
  • Peer Reviewed / Open Access
• [Journal Article] Renal Impairment in Chronic Hepatitis B: A Review (2018)
  • Author(s): Kamimura Hiroteru、Setsu Toru、Kimura Naruhiro、Yokoo Takeshi、Sakamaki Akira、Kamimura Kenya、Tsuchiya Atsunori、Takamura Masaaki、Yamagiwa Satoshi、Terai Shuji
  • Journal Title: Diseases Volume: 6 Issue: 2 Pages: 52-52
  • DOI: 10.3390/diseases6020052
  • Peer Reviewed / Open Access
• [Journal Article] Rapidly declining skeletal muscle mass predicts poor prognosis of hepatocellular carcinoma treated with transcatheter intra-arterial therapies (2018)
  • Author(s): Kobayashi Takamasa、Kawai Hirokazu、Nakano Oki、Abe Satoshi、Kamimura Hiroteru、Sakamaki Akira、Kamimura Kenya、Tsuchiya Atsunori、Takamura Masaaki、Yamagiwa Satoshi、Terai Shuji
  • Journal Title: BMC Cancer Volume: 18 Issue: 1 Pages: 756-756
  • DOI: 10.1186/s12885-018-4673-2
  • Peer Reviewed / Open Access
• [Journal Article] Prognostic value of subcutaneous adipose tissue volume in hepatocellular carcinoma treated with transcatheter intra-arterial therapy (2018)
  • Author(s): Kobayashi Takamasa、Kawai Hirokazu、Nakano Oki、Abe Satoshi、Kamimura Hiroteru、Sakamaki Akira、Kamimura Kenya、Tsuchiya Atsunori、Takamura Masaaki、Yamagiwa Satoshi、Terai Shuji
  • Journal Title: Cancer Management and Research Volume: Volume 10 Pages: 2231-2239
  • DOI: 10.2147/cmar.s167417
  • Peer Reviewed / Open Access
• [Journal Article] Diagnosis and management of fibromuscular dysplasia and segmental arterial mediolysis in gastroenterology field: A mini-review (2018)
  • Author(s): Ko Masayoshi、Kamimura Kenya、Ogawa Kohei、Tominaga Kentaro、Sakamaki Akira、Kamimura Hiroteru、Abe Satoshi、Mizuno Kenichi、Terai Shuji
  • Journal Title: World Journal of Gastroenterology Volume: 24 Issue: 32 Pages: 3637-3649
  • DOI: 10.3748/wjg.v24.i32.3637
  • Peer Reviewed / Open Access
• [Journal Article] Gene Therapy for Pancreatic Diseases: Current Status (2018)
  • Author(s): Kamimura Kenya、Yokoo Takeshi、Terai Shuji
  • Journal Title: International Journal of Molecular Sciences Volume: 19 Issue: 11 Pages: 3415-3415
  • DOI: 10.3390/ijms19113415
  • Peer Reviewed / Open Access
• [Journal Article] Efficacy and safety of pancreas-targeted hydrodynamic gene delivery in rats. (2017)
  • Author(s): Ogawa K, Kamimura K (contributed equally and corresponding), Kobayashi Y, Abe H, Yokoo T, Sakai N, Nagoya T, Sakamaki A, Abe S, Hayashi K, Ikarashi S, Kohisa J, Tsuchida M, Aoyagi Y, Zhang G, Liu D, Terai S.
  • Journal Title: Mol Ther Nucleic Acids. Volume: 9 Pages: 80-88
  • DOI: 10.1016/j.omtn.2017.08.009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Spontaneous regression of hepatocellular carcinoma: A mini-review. (2017)
  • Author(s): Sakamaki A, Kamimura K, Abe S, Tsuchiya A, Takamura M, Kawai H, Yamagiwa S, Terai S.
  • Journal Title: World J Gastroenterol Volume: 23 Issue: 21 Pages: 3797-3804
  • DOI: 10.3748/wjg.v23.i21.3797
  • Peer Reviewed / Open Access
• [Journal Article] Transhepatic arterial infusion chemotherapy using a combination of miriplatin and CDDP powder versus miriplatin alone in the treatment of hepatocellular carcinoma: a randomized controlled trial (2017)
  • Author(s): Kamimura Kenya、Suda Takeshi、Yokoo Takeshi、Kamimura Hiroteru、Kanefuji Tsutomu、Tsuchiya Atsunori、Takamura Masaaki、Kawai Hirokazu、Waguri Nobuo、Yamagiwa Satoshi、Terai Shuji
  • Journal Title: BMC Cancer Volume: 17 Issue: 1 Pages: 322-322
  • DOI: 10.1186/s12885-017-3320-7
  • Peer Reviewed / Open Access
• [Journal Article] Tumor markers for early diagnosis for brain metastasis of hepatocellular carcinoma: A case series and literature review for effective loco-regional treatment (2017)
  • Author(s): Kamimura Kenya、Kobayashi Yuji、Takahashi Yoshifumi、Abe Hiroyuki、Kumaki Daisuke、Yokoo Takeshi、Kamimura Hiroteru、Sakai Norihiro、Sakamaki Akira、Abe Satoshi、Takamura Masaaki、Kawai Hirokazu、Yamagiwa Satoshi、Terai Shuji
  • Journal Title: Cancer Biology & Therapy Volume: 18 Issue: 2 Pages: 79-84
  • DOI: 10.1080/15384047.2016.1276134
  • Peer Reviewed
• [Journal Article] Drug-eluting beads in hepatocellular carcinoma (2017)
  • Author(s): Abe Hiroyuki、Kamimura Kenya、Terai Shuji
  • Journal Title: Therapeutic Advances in Gastroenterology Volume: 10 Issue: 3 Pages: 337-338
  • DOI: 10.1177/1756283x16681710
  • Peer Reviewed
• [Presentation] Development of Hydrodynamic Gene Therapy; Assessment of Liver-targeted Hydrodynamic Gene Delivery in Large Animal Disease Model (2019)
  • Author(s): Kenya Kamimura, et al.
  • Organizer: JAPAN SOCIETY OF GENE AND CELL THERAPY 2019
  • Int'l Joint Research
• [Presentation] 肝癌に対する分子標的薬の有害事象の発症予測 (2019)
  • Author(s): 上村顕也、他。
  • Organizer: 第55回日本肝臓学会総会
• [Presentation] 肝癌に対する分子標的治療中のヒスチジンによる肝予備能維持効果と副作用予防 (2019)
  • Author(s): 上村顕也 他。
  • Organizer: 第23回 日本肝臓学会大会
• [Presentation] Effective Prevention of Sorafenib-Related Adverse Events and Maintenance of Hepatic Function by Histidine (2019)
  • Author(s): Kenya Kamimura, et al.
  • Organizer: The American Association for the Study of Liver Diseases 2019
  • Int'l Joint Research
• [Presentation] NASH/NAFLDの病態における自律神経を介した消化管ホルモンの関与 (2019)
  • Author(s): 上村顕也、他。
  • Organizer: 第55回日本肝臓学会総会
• [Presentation] Effect of gastrointestinal hormone on liver regeneration via neuronal relay (2019)
  • Author(s): Kenya Kamimura, et al.
  • Organizer: GUTS FORUM 2019
• [Presentation] SGLT2阻害薬によるNASH進展抑制効果の検証：メダカNASHモデルを用いて (2019)
  • Author(s): 上村顕也、他。
  • Organizer: 第105回日本消化器病学会総会
• [Presentation] マウス肝線維化進展予防薬の探索及びレトロゾールによる線維化進展予防効果の検証 (2019)
  • Author(s): 上村顕也、他。
  • Organizer: 第105回日本消化器病学会総会
• [Presentation] 肝癌に対する放射線治療の検討 (2019)
  • Author(s): 上村顕也、他。
  • Organizer: 第55回日本肝臓学会総会
• [Presentation] 肝原発悪性腫瘍に対する放射線治療への期待－内科医の立場から (2019)
  • Author(s): 上村顕也
  • Organizer: 第28回日本定位放射線治療学会
• [Presentation] 肝癌の局所制御及び集学的治療法としての放射線治療の検討 (2019)
  • Author(s): 上村顕也、他。
  • Organizer: 第43回日本肝臓学会西部会
• [Presentation] 遺伝子治療の現況と新規遺伝子治療法開発に向けた基礎及び前臨床研究 (2019)
  • Author(s): 上村顕也
  • Organizer: 東海医学会
  • Invited
• [Presentation] Liver-targeted Hydrodynamic Gene Delivery in Hemophiliac Dogs (2018)
  • Author(s): 上村顕也
  • Organizer: 第３回日本遺伝子細胞治療学会若手研究会セミナー
• [Presentation] Assessments of Liver-Targeted Hydrodynamic Gene Delivery in Hemophiliac Dogs (2018)
  • Author(s): Kamimura K, et al.
  • Organizer: 全米遺伝子細胞治療学会
  • Int'l Joint Research
• [Presentation] 手足症候群に対するヒスチジンの効果 (2018)
  • Author(s): 上村顕也
  • Organizer: 第１８回 日本肝がん分子標的治療研究会
• [Presentation] 自律神経線維による肝，脳，腸の臓器間ネットワークを介した肝切除後の肝再生メカニズムの検討 (2018)
  • Author(s): 上村顕也
  • Organizer: 日本消化器関連学会週間 JDDW2018
• [Presentation] 肝疾患における自律神経による臓器間ネットワークの関与 (2018)
  • Author(s): 上村顕也
  • Organizer: 42回日本肝臓学会東部会
• [Presentation] Development of Safe and Effective Hydrodynamic Gene Therapy for Liver Diseases - Effects of Fibrotic Tissue on Gene Delivery Efficiency (2017)
  • Author(s): Kenya Kamimura1, Yuji Kobayashi1, Hiroyuki Abe1, Takeshi Yokoo1, Masato Ohtsuka2,3, Hiromi Miura4, Guisheng Zhang5, Dexi Liu5, and Shuji Terai1
  • Organizer: 第23回日本遺伝子治療学会
  • Int'l Joint Research
• [Presentation] 肝硬変合併肝癌への遺伝子治療確立に向けた方法論の検討 (2017)
  • Author(s): 上村顕也、横尾健、阿部寛幸、小林雄司、小川光平、品川陽子、井上良介、酒井規裕、名古屋卓郎、山際 訓、寺井崇二
  • Organizer: 第59回日本消化器病学会大会
• [Book] Nucleic acid-based therapy: Development of a non-viral-based delivery approach in In Vivo and Ex Vivo Gene Therapy for Inherited and Non-Inherited Disorders (2019)
  • Author(s): Takeshi Yokoo, Kenya Kamimura, Tsutomu Kanefuji, Takeshi Suda and Shuji Terai
  • Total Pages: 18
  • Publisher: IntechOpen Limited
• [Book] The Management of Constipation: Current Status and Future Prospects in Constipation (2019)
  • Author(s): Maruyama M, Kamimura K, Sugita M, Nakajima N, Takahashi Y, Isokawa O, Terai S
  • Total Pages: 16
  • Publisher: IntechOpen Limited
• [Remarks] 肝がんに対する遺伝子治療法を開発 －肝がん細胞に毒素遺伝子を発現し、治療効果と安全性を明らかに－
  • URL: https://www.med.niigata-u.ac.jp/contents/info/news_topics/121_index.html
• [Patent(Industrial Property Rights)] レトロゾールの線維化抑制効果 (2018)
  • Inventor(s): 上村顕也、寺井崇二、酒井規裕
  • Industrial Property Rights Holder: 上村顕也、寺井崇二、酒井規裕
  • Industrial Property Rights Type: 特許
  • Filing Date: 2018
• [Patent(Industrial Property Rights)] ジフテリアトキシンＡサブユニット発現ＤＮＡプラスミドによる肝癌遺伝子治療 (2017)
  • Inventor(s): 上村顕也
  • Industrial Property Rights Holder: 上村顕也
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-080417
  • Filing Date: 2017
• [Patent(Industrial Property Rights)] 膵臓選択的なハイドロダイナミック遺伝子導入法 (2017)
  • Inventor(s): 上村顕也
  • Industrial Property Rights Holder: 上村顕也
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-172377
  • Filing Date: 2017