Analysis of HBsAg and its immune response with novel high sensitivity method
| Project/Area Number |
17K09410
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | University of Toyama |
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Principal Investigator |
Tajiri Kazuto 富山大学, 学術研究部医学系, 准教授 (30512165)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | HBV感染症 / HBs抗原 / L蛋白 / B型肝炎表面抗原 / B型肝炎ウイルス / 抗原検出法 / HBｓ抗原 / モノクローナル抗体 |
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| Outline of Final Research Achievements |
We evaluated serum of patients with HBV infection by monoclonal antibodies for small-HBsAg, glycosylated middle-HBsAg and large-HBsAg, those were obtained our novel system that can effectively detect antigen-specific cells. As the results, large-HBsAg was found independent of titer of HBV-DNA or HBsAg which is recognized with polyclonal antibody and is commercially available. Our results indicate that large HBsAg has a specific role in HBsAg biology. Our novel HBsAg detection system might provide a new insight in the study of HBV infection.
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| Academic Significance and Societal Importance of the Research Achievements |
HBV感染症においてはHBV-DNAの制御が可能となったが、HBVにより引き起こされる病態は持続するため、真のHBV感染症のコントロールにはHBｓAgの低下が重要とされてきた。しかし、HBｓAgの現在の測定系はポリクローナル抗体を用いたおおまかな量の測定しかできていない。我々のモノクローナル抗体を用いたHBsAgの測定はHBsAgの質的解析を行うことができ、HBV感染症の病態をより反映したHBsAg測定系となりうる。
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Report
(5 results)
Research Products
(11 results)
