Reversible and irreversible damages of hepatocyte after DAA treatment for HCV carriers

• Project/Area Number: 17K09447
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Wakita Takaji 国立感染症研究所, 所長, 所長 (40280789)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: HCV / ＨＣＶ

Outline of Final Research Achievements

Development of DAA for HCV has dramatically changed its prognosis. On the other hand, persistence of liver damage after elimination of virus and HCC are problems after viral clearance. In this study, we analyzed the ultrastructural changes and gene expression changes in liver injury using HCV infection models. HCV uninfected, HCV infected and post-HCV infected excluded cells were constructed. In these cells, there were genes that changed reversibly or irreversibly in the process of HCV infection and HCV elimination. For further study, analysis of human liver chimera mice and patient specimens reveals genes that reversibly and irreversibly change in common in the three models, and thus liver damage after DAA treatment.

Academic Significance and Societal Importance of the Research Achievements

ＨＣＶ感染は肝臓に持続感染して慢性肝炎、肝硬変、肝臓癌といった慢性肝障害を引き起こす。慢性肝障害は肝細胞のアポトーシス、リンパ球浸潤、脂肪肝などを伴う。さらに線維化が進行すると肝小葉が再構築され、肝臓の血液循環動態も変化する。従って、ＨＣＶ持続感染による肝障害はウイルス感染そのもの、および宿主の免疫反応だけによるものではなく、肝臓の組織再構築による変化も関与することが考えられる。従って、DAA治療によりウイルス排除が達成された後も、引き続き肝障害が持続し、肝発がんに至ることもあり得る。

Research Products

• [Journal Article] Establishment of infectious genotype 4 cell culture-derived hepatitis C virus (2020)
  • Author(s): Watanabe Noriyuki、Suzuki Takaya、Date Tomoko、Hussan Hussein Aly、Hmwe Su Su、Aizaki Hideki、Sugiyama Masaya、Mizokami Masashi、Delaney IV William、Cheng Guofeng、Muramatsu Masamichi、Wakita Takaji
  • Journal Title: Journal of General Virology Volume: 101 Issue: 2 Pages: 188-197
  • DOI: 10.1099/jgv.0.001378
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Surfeit 4 contributes to the replication of hepatitis C virus using double membrane vesicles. (2020)
  • Author(s): Kong L, Aoyagi H, Yang Z, Ouyang T, Matsuda M, Fujimoto A, Watashi K, Suzuki R, Arita M, Yamagoe S, Dohmae N, Suzuki T, Suzuki T, Muramatsu M, Wakita T, Aizaki H.
  • Journal Title: Journal of Virology Volume: 94 Issue: 2
  • DOI: 10.1128/jvi.00858-19
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The Beginning of Ending Hepatitis C Virus: A Summary of the 26th International Symposium on Hepatitis C Virus and Related Viruses (2020)
  • Author(s): Shin Eui-Cheol、Han Ji Won、Kang Wonseok、Kato Takanobu、Kim Seong-Jun、Zhong Jin、Kim Seungtaek、Park Su-Hyung、Sung Pil Soo、Watashi Koichi、Park Jun Yong、Windisch Marc P.、Oh Jong-Won、Wakita Takaji、Han Kwang-Hyub、Jang Sung Key
  • Journal Title: Viruses Volume: 12 Issue: 3 Pages: 302-302
  • DOI: 10.3390/v12030302
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ledipasvir and Sofosbuvir for Acute Hepatitis C Virus Monoinfection Associated with a High Risk of Acute Liver Failure (2019)
  • Author(s): Hatanaka Takeshi、Naganuma Atsushi、Tateyama Yumeo、Yoshinari Fukiko、Hoshino Takashi、Sato Ken、Hmwe Su Su、Aizaki Hideki、Wakita Takaji、Kakizaki Satoru、Uraoka Toshio
  • Journal Title: Internal Medicine Volume: 58 Issue: 20 Pages: 2969-2975
  • DOI: 10.2169/internalmedicine.2982-19
  • NAID: 130007728784
  • ISSN: 0918-2918, 1349-7235
  • Year and Date: 2019-10-15
  • Peer Reviewed
• [Journal Article] Inhibition Mechanisms of Hepatitis C Virus Infection by Caffeic Acid and Tannic Acid (2019)
  • Author(s): Shirasago Yoshitaka、Inamori Yoko、Suzuki Takeru、Tanida Isei、Suzuki Tetsuro、Sugiyama Kazuo、Wakita Takaji、Hanada Kentaro、Fukasawa Masayoshi
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 42 Issue: 5 Pages: 770-777
  • DOI: 10.1248/bpb.b18-00970
  • NAID: 130007641446
  • ISSN: 0918-6158, 1347-5215
  • Year and Date: 2019-05-01
  • Peer Reviewed
• [Journal Article] Bardoxolone methyl as a novel potent antiviral agent against hepatitis B and C viruses in human hepatocyte cell culture systems. (2019)
  • Author(s): Nio Yasunori、Sasai Machiko、Akahori Yuichi、Okamura Hitomi、Hasegawa Hikari、Oshima Mizuki、Watashi Koichi、Wakita Takaji、Ryo Akihide、Tanaka Yasuhito、Hijikata Makoto
  • Journal Title: Antiviral Research Volume: 169 Pages: 104537-104537
  • DOI: 10.1016/j.antiviral.2019.104537
  • Peer Reviewed
• [Journal Article] Human-rat chimeric anti-occludin monoclonal antibodies inhibit hepatitis C virus infection (2019)
  • Author(s): Shimizu Yoshimi、Yoneda Kohei、Shirasago Yoshitaka、Suzuki Takeru、Tada Minoru、Ishii-Watabe Akiko、Sugiyama Kazuo、Suzuki Tetsuro、Wakita Takaji、Yagi Kiyohito、Kondoh Masuo、Fukasawa Masayoshi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 514 Issue: 3 Pages: 785-790
  • DOI: 10.1016/j.bbrc.2019.05.019
  • Peer Reviewed
• [Journal Article] miR-27b-mediated suppression of aquaporin-11 expression in hepatocytes reduces HCV genomic RNA levels but not viral titers (2019)
  • Author(s): Sakurai Fuminori、Hashimoto Rina、Inoue Chieko、Wakabayashi Keisaku、Tsukamoto Tomohito、Imaizumi Tsutomu、Andres Taracena Gandara Marcos、Sakai Eiko、Itsuki Kanae、Sakamoto Naoya、Wakita Takaji、Mizuguchi Hiroyuki
  • Journal Title: Virology Journal Volume: 16 Issue: 1 Pages: 58-58
  • DOI: 10.1186/s12985-019-1160-6
  • Peer Reviewed
• [Journal Article] Establishment of Replication-Competent HCV Strain with Minimum Modifications (2019)
  • Author(s): Kato Takanobu、Murayama Asako、Wakita Takaji
  • Journal Title: Methods Mol Biol. Volume: 1911 Pages: 73-83
  • DOI: 10.1007/978-1-4939-8976-8_4
  • ISBN: 9781493989751, 9781493989768
• [Journal Article] Cell Culture Systems of HCV Using JFH-1 and Other Strains (2019)
  • Author(s): Wakita Takaji
  • Journal Title: Cold Spring Harbor Perspectives in Medicine Volume: 9 Issue: 11 Pages: a036806-a036806
  • DOI: 10.1101/cshperspect.a036806
• [Journal Article] The aryl hydrocarbon receptor?cytochrome P450 1A1 pathway controls lipid accumulation and enhances the permissiveness for hepatitis C virus assembly (2018)
  • Author(s): Ohashi Hirofumi、Nishioka Kazane、Nakajima Syo、Kim Sulyi、Suzuki Ryosuke、Aizaki Hideki、Fukasawa Masayoshi、Kamisuki Shinji、Sugawara Fumio、Ohtani Naoko、Muramatsu Masamichi、Wakita Takaji、Watashi Koichi
  • Journal Title: Journal of Biological Chemistry Volume: 293 Issue: 51 Pages: 19559-19571
  • DOI: 10.1074/jbc.ra118.005033
  • Peer Reviewed
• [Journal Article] Thermostable hepatitis C virus JFH1-derived variant isolated by adaptation to Huh7.5.1 cells (2018)
  • Author(s): Shirasago Yoshitaka、Fukazawa Hidesuke、Aizaki Hideki、Suzuki Tetsuro、Suzuki Takeru、Sugiyama Kazuo、Wakita Takaji、Hanada Kentaro、Abe Ryo、Fukasawa Masayoshi
  • Journal Title: Journal of General Virology Volume: 99 Issue: 10 Pages: 1407-1417
  • DOI: 10.1099/jgv.0.001117
  • Peer Reviewed
• [Journal Article] Evaluation of in vitro screening and diagnostic kits for hepatitis C virus infection (2018)
  • Author(s): Momose Haruka、Matsuoka Sahoko、Murayama Asako、Yamada Norie、Okuma Kazu、Ikebe Emi、Hoshi Yuji、Muramatsu Masamichi、Wakita Takaji、Toyota Kuro、Kato Takanobu、Hamaguchi Isao
  • Journal Title: Journal of Clinical Virology Volume: 105 Pages: 97-102
  • DOI: 10.1016/j.jcv.2018.06.008
  • Peer Reviewed
• [Journal Article] Expression of a functional intrabody against hepatitis C virus core protein in Escherichia coli and silkworm pupae (2018)
  • Author(s): Kato Tatsuya、Hasegawa Moeko、Yamamoto Takeshi、Miyazaki Takatsugu、Suzuki Ryosuke、Wakita Takaji、Suzuki Tetsuro、Park Enoch Y.
  • Journal Title: Protein Expression and Purification Volume: 150 Pages: 61-66
  • DOI: 10.1016/j.pep.2018.05.009
  • NAID: 120006543634
  • Peer Reviewed
• [Journal Article] ULBP1 is induced by hepatitis C virus infection and is the target of the NK cell-mediated innate immune response in human hepatocytes (2018)
  • Author(s): Dansako Hiromichi、Imai Hirotaka、Ueda Youki、Satoh Shinya、Wakita Takaji、Kato Nobuyuki
  • Journal Title: FEBS Open Bio Volume: 8 Issue: 3 Pages: 361-371
  • DOI: 10.1002/2211-5463.12373
  • Peer Reviewed / Open Access
• [Journal Article] Critical challenges and emerging opportunities in hepatitis C virus research in an era of potent antiviral therapy: Considerations for scientists and funding agencies (2018)
  • Author(s): Bartenschlager Ralf、Baumert Thomas F.、Bukh Jens、Houghton Michael、Lemon Stanley M.、Lindenbach Brett D.、Lohmann Volker、Moradpour Darius、Pietschmann Thomas、Rice Charles M.、Thimme Robert、Wakita Takaji
  • Journal Title: Virus Research Volume: 248 Pages: 53-62
  • DOI: 10.1016/j.virusres.2018.02.016
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Monoclonal Antibodies against Occludin Completely Prevented Hepatitis C Virus Infection in a Mouse Model (2018)
  • Author(s): Shimizu Yoshimi、Shirasago Yoshitaka、Kondoh Masuo、Suzuki Tetsuro、Wakita Takaji、Hanada Kentaro、Yagi Kiyohito、Fukasawa Masayoshi
  • Journal Title: Journal of Virology Volume: 92 Issue: 8
  • DOI: 10.1128/jvi.02258-17
  • Peer Reviewed
• [Journal Article] Interferon sensitivity-determining region of hepatitis C virus influences virus production and interferon signaling (2017)
  • Author(s): Sugiyama R., Murayama A., Nitta S., Yamada N., Tasaka-Fujita M., Masaki T., Aly H. H., Shiina M., Ryo A., Ishii K., Wakita T. and Kato T.
  • Journal Title: Oncotarget Volume: 9 Issue: 5 Pages: 5627-5640
  • DOI: 10.18632/oncotarget.23562
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of PUF60 in Transcriptional and Post-transcriptional Regulation of Hepatitis B Virus Pregenomic RNA Expression (2017)
  • Author(s): Sun Suofeng、Nakashima Kenji、Ito Masahiko、Li Yuan、Chida Takeshi、Takahashi Hirotaka、Watashi Koichi、Sawasaki Tatsuya、Wakita Takaji、Suzuki Tetsuro
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 12874-12874
  • DOI: 10.1038/s41598-017-12497-y
  • Peer Reviewed / Open Access
• [Journal Article] Development of hepatoma-derived, bidirectional oval-like cells as a model to study host interactions with hepatitis C virus during differentiation (2017)
  • Author(s): Ito Masahiko、Sun Suofeng、Fukuhara Takasuke、Suzuki Ryosuke、Tamai Miho、Yamauchi Toyohiko、Nakashima Kenji、Tagawa Yoh-ichi、Okazaki Shigetoshi、Matsuura Yoshiharu、Wakita Takaji、Suzuki Tetsuro
  • Journal Title: Oncotarget Volume: 8 Issue: 33 Pages: 53899-53915
  • DOI: 10.18632/oncotarget.19108
  • Peer Reviewed / Open Access
• [Journal Article] Hepatitis C virus-induced innate immune responses in human iPS cell-derived hepatocyte-like cells (2017)
  • Author(s): Sakurai Fuminori、Kunito Takemaru、Takayama Kazuo、Hashimoto Rina、Tachibana Masashi、Sakamoto Naoya、Wakita Takaji、Mizuguchi Hiroyuki
  • Journal Title: Virus Research Volume: 242 Pages: 7-15
  • DOI: 10.1016/j.virusres.2017.09.004
  • Peer Reviewed
• [Journal Article] Reply to Padmanabhan and Dixit: Hepatitis C virus entry inhibitors for optimally boosting direct-acting antiviral-based treatments. (2017)
  • Author(s): Ohashi HK, Koizumi Y, Fukano K, Wakita T, Perelson AS, Iwami S, Watashi K
  • Journal Title: Proc Acad Natl Acad USA Volume: 114 Issue: 23
  • DOI: 10.1073/pnas.1705234114
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of miR-122-independent propagation of HCV (2017)
  • Author(s): C. Ono, T. Fukuhara, D. Motooka, S. Nakamura, D. Okuzaki, S. Yamamoto, T. Tamura, H. Mori, A. Sato, K. Uemura, Y. Fauzyah, T. Kurihara, T. Suda, A. Nishio, Su Su Hmwe, T. Okamoto, T. Tatsumi, T. Takehara, K. Chayama, T. Wakita, K. Koike, Y. Matsuura
  • Journal Title: PLoS Pathogens Volume: - Issue: 5 Pages: e1006374-e1006374
  • DOI: 10.1371/journal.ppat.1006374
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Hepatitis C virus triggers Golgi fragmentation and autophagy through the immunity-related GTPase M (2017)
  • Author(s): Hansen Marianne D.、Johnsen Ingvild B.、Stiberg Kim A.、Sherstova Tatyana、Wakita Takaji、Richard Gabriel Mary、Kandasamy Richard K.、Meurs Eliane F.、Anthonsen Marit W.
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 114 Issue: 17
  • DOI: 10.1073/pnas.1616683114
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Ｃ型肝炎の治療戦略と病態の解明 (2019)
  • Author(s): 脇田隆字、相崎英樹、渡士幸一
  • Organizer: 第105回日本消化器病学会総会
  • Invited
• [Presentation] Ｃ型肝炎ウイルス研究 (2019)
  • Author(s): 脇田隆字
  • Organizer: 第67回日本ウイルス学会学術集会
  • Invited
• [Presentation] Identification and functional analysis of aryl hydrocarbon receptor ligands reducing lipid droplets and hepatitis C virus (2019)
  • Author(s): Kazane NISHIOKA, Hirofumi OHASHI, Ryosuke SUZUKI, Hideki AIZAKI, Masamichi MURAMATSU, Takaji Wakita, Koichi WATASHI
  • Organizer: 第67回日本ウイルス学会学術集会
• [Presentation] Role of aryl hydrocarbon receptor-dependent lipid production in the maintenance of hepatitis C virus infection and the emergence of drug resistant viruses (2019)
  • Author(s): Hirofumi Ohashi, Ryosuke Suzuki, Hideki Aizaki, Masayoshi Fukasawa, Kouji Kuramochi, Masamichi Muramatsu, Takaji Wakita, Koichi Watashi
  • Organizer: 第67回日本ウイルス学会学術集会
• [Presentation] Abnormal hepatocellular organelles are still present in patients with sustained virological response (SVR) (2019)
  • Author(s): Haruyo Aoyagi, Hiroko Iijima, Minami Kikuchi, Maiko Koyama, Mami Matsuda, Koichi Watashi, Ryo suke Suzuki, Takahiro Masaki, Noritomo Shimada, Keizo Kato, Akihito Tsubota, Kenjiro Wake, Takaji Wakita, Hideki Aizaki
  • Organizer: 26th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Establishment of neutralizing E2 antibody from E2-immunized mouse. (2019)
  • Author(s): Minami Yoshida, Noriyuki Watanabe, Hussein H Aly, Madoka Koyanagi, Yutaka Arimura, Hideki Aizaki, Masamichi Muramatsu, Takaji Wakita
  • Organizer: 26th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] The inhibition of AhR-dependent lipid accumulation pathway impacted the host permissiveness to HCV production and lead to elimination (2019)
  • Author(s): Hirofumi Ohashi, Ryosuke Suzuki, Hideki Aizaki, Masayoshi Fukasawa, Kouji Kuramochi, Masamichi Muramatsu, Takaji Wakita, Koichi Watashi
  • Organizer: 26th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Identification of aryl hydrocarbon receptor ligands as inhibitor of lipid accumulation and hepatitis C virus production (2019)
  • Author(s): Kazane NISHIOKA, Hirofumi OHASHI, Ryosuke SUZUKI, Hideki AIZAKI, Masamichi MURAMATSU, Takaji Wakita, Koichi WATASHI
  • Organizer: 26th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Screening of anti-hepatitis C virus natural compounds extracted from crude drugs using an in vitro visualization system (2019)
  • Author(s): Xin Zheng, Kousho Wakae, Noriyuki Watanabe, Aly H. Hussein, Koichi Watashi, Ryosuke Suzuki, Hideki Aizaki, Takaji Wakita, Shaojiang Song, Masamichi Muramatsu
  • Organizer: 26th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] C型慢性肝炎のウイルス学的著効後の肝組織のウイルス遺伝子 (2018)
  • Author(s): 相崎英樹、脇田隆字
  • Organizer: 第54回日本肝臓学会総会
• [Presentation] 感染性を有する4型C型肝炎ウイルスの樹立 (2018)
  • Author(s): 渡邉則幸、鈴木貴也、伊達朋子、スス・ムェー、Hussein H Aly、相崎英樹、村松正道、 脇田隆字
  • Organizer: 第28回抗ウイルス療法学会学術集会
• [Presentation] Hepatitis C virus infection triggers the transactivation of aryl hydrocarbon receptor to rearrange hepatic lipid biosynthesis (2018)
  • Author(s): Hirofumi Ohashi, Syo Nakajima, Sulyi Kim, Ryosuke Suzuki, Hideki Aizaki, Masayoshi Fukasawa, Shinji Kamisuki, Fumio Sugawara, Naoko Ohtani, Masamichi Muramatsu, Takaji Wakita, Koichi Watashi
  • Organizer: 第66回日本ウイルス学会学術集会
• [Presentation] Abnormal hepatocellular organelles remain to be observed in sustained virological response (SVR) patients (2018)
  • Author(s): Haruyo Aoyagi, Hiroko Iijima, Xin Zheng, Yu Ting Kao, Gewaid E. Hossam, Mami Matsuda, Koichi Watashi, Ryosuke Suzuki, Takahiro Masaki, Noritomo Shimada, Keizo Kato, Akihito Tsubota, Ayako Mimata, Yuriko Sakamaki, Shizuko Ichinose, Kenjiro Wake, Takaji Wakita, Hideki Aizaki.
  • Organizer: 第66回日本ウイルス学会学術集会
• [Presentation] Identification of aryl hydrocarbon receptor ligands inhibiting the lipid accumulation and hepatitis C virus production (2018)
  • Author(s): Kazane NISHIOKA , Hirofumi OHASHI, Ryosuke SUZUKI, Hideki AIZAKI, Masamichi MURAMATSU, Takaji WAKITA, Koichi WATASHI
  • Organizer: 第66回日本ウイルス学会学術集会
• [Presentation] Construction of chimeric reporter HCV with efficient production capacity (2018)
  • Author(s): Rika Sato, Noriyuki Watanabe, Hussein H Aly, Madoka Koyanagi, Yutaka Arimura, Hideki Aizaki, Masamichi Muramatsu, Takaji Wakita
  • Organizer: 第66回日本ウイルス学会学術集会
• [Presentation] 感染性を有する4型C型肝炎ウイルスの樹立 (2018)
  • Author(s): 渡邉則幸、鈴木貴也、伊達朋子、スス・ムェー、Hussein H Aly、相崎英樹、村松正道、脇田隆字
  • Organizer: 第66回日本ウイルス学会学術集会
• [Presentation] SVR後の肝組織のウイルス遺伝子およびオルガネラ異常の残存 (2018)
  • Author(s): 相崎英樹、村松正道、脇田隆字
  • Organizer: 第42回日本肝臓学会東部会
• [Presentation] Antiviral activity of phospholipase A2 group V (PLA2G5) against HCV (2018)
  • Author(s): Francesc PUIG-BASAGOITI, Masayoshi FUKASAWA, Ryosuke SUZUKI, Koichi WATASHI, Masamichi MURAMATSU, Takaji WAKITA, Hideki AIZAKI
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Aryl hydrocarbon receptor-cytochrome p450 1a1 pathway regulates hepatic lipid biosynthesis to maximize hepatitis C virus production (2018)
  • Author(s): Hirofumi Ohashi, Syo Nakajima, Sulyi Kim, Ryosuke Suzuki, Hideki Aizaki, Masayoshi Fukasawa, Shinji Kamisuki, Kouji Kuramochi, Fumio Sugawara, Naoko Ohtani, Masamichi Muramatsu, Takaji Wakita, Koichi Watashi
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Aryl hydrocarbon receptor ligands inhibiting the lipid accumulation and hepatitis C virus production (2018)
  • Author(s): Kazane Nishioka, Hirofumi Ohashi, Ryosuke Suzuki, Hideki Aizaki, Kouji Kuramochi, Masamichi Muramatsu, Takaji Wakita, Koichi Watashi,
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Flavivirus subviral particles-based DNA vaccine induces neutralizing antibodies against HCV (2018)
  • Author(s): Keigo Yato, Mami Matsuda, Noriyuki Watanabe, Shogo Nakajima, Akira Fujimoto, Koichi Watashi, Hideki Aizaki, Takanobu Kato, Koji Tamura, Masamichi Muramatsu, Takaji Wakita, Ryosuke Suzuki
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Construction of chimeric reporter HCV with efficient production capacity (2018)
  • Author(s): Rika Sato, Noriyuki Watanabe, Hussein H Aly, Madoka Koyanagi, Yutaka Arimura, Hideki Aizaki, Masamichi Muramatsu, Takaji Wakita
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses
• [Presentation] Establishment of infectious genotype 4a HCVcc (2018)
  • Author(s): Noriyuki Watanabe, Takaya Suzuki, Tomoko Date, Su Su Hmwe, Hussein H Aly, Hideki Aizaki, Masaya Sugiyama, Masashi Mizokami, Mohamed El Kassas, Ashraf Tabl, William IV Delaney, Guofeng Cheng, Masamichi Muramatsu, Takaji Wakita
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Optimization of hepatitis C virus treatment and post SVR syndrome (2018)
  • Author(s): Takaji Wakita
  • Organizer: 20th International Conference on Emerging Infectious Diseases in the Pacific Rim
  • Int'l Joint Research / Invited
• [Presentation] Ｃ型肝炎ウイルス基礎研究の過去、現在、未来 (2018)
  • Author(s): 脇田隆字、相崎英樹、渡士幸一
  • Organizer: 第122回日本消化器病学会北海道支部例会
  • Invited
• [Presentation] Hepatitis C virus replication, treatment optimization and post SVR syndrome (2018)
  • Author(s): Takaji Wakita
  • Organizer: Annual Conference of the Asian pacific Association for the Study of the Liver
  • Int'l Joint Research / Invited
• [Presentation] ウイルス培養系を用いた肝炎ウイルス研究 (2017)
  • Author(s): 脇田隆字、相崎英樹
  • Organizer: 第53回日本肝臓学会総会
  • Invited
• [Presentation] Molecular mechanisms of HCV membrane replication complex formation (2017)
  • Author(s): Takaji Wakita, Lingbao Kong, Hossam Gewaid, Zheng, Xin, Hideki Aizaki
  • Organizer: International Conference of the Korean Society for Molecular and Cellular Biology
  • Int'l Joint Research / Invited
• [Presentation] 最近の目覚ましいＣ型肝炎治療への道のりとその後 (2017)
  • Author(s): 脇田隆字
  • Organizer: 中部工大医工融合健康プログラム
  • Invited
• [Presentation] Induction of neutralization E2 antibody by immunization of recombinant E2 proteins (2017)
  • Author(s): 渡邉則幸、伊達朋子、フセイン・アリ、相崎英樹、脇田隆字
  • Organizer: 第65回日本ウイルス学会学術集会
• [Presentation] C型慢性肝炎のウイルス学的著効（SVR）後の肝実質細胞のオルガネラ異常の解析 (2017)
  • Author(s): 青柳東代, 飯島尋子, Francesc Puig-Basagoiti, Zheng Xin, Yu Ting Kao, Gewaid E. Hossam, 在津拓馬, 松田麻未, 渡士幸一, 鈴木亮介, 政木隆博, 島田紀明, 加藤慶三, 坪田昭人, 三又絢子, 酒巻有里子, 市野瀬志津子, 和氣健二郎, 脇田隆字, 相崎英樹
  • Organizer: 第27回抗ウイルス療法学会学術集会
• [Presentation] Abnormal organelles were continuously observed in sustained virological response (SVR) patients (2017)
  • Author(s): Aoyagi H, Iijima H, Puig-Basagoiti F, Zheng X, Kao YT, Gewaid HE, Zaitsu T, Matsuda M, Watashi K, Suzuki R, Masaki T, Shimada N, Kato K, Tsubota A, Mimata A, Sakamaki Y, Ichinose S, Wake K, Wakita T, Aizaki H
  • Organizer: he Asian Pacific Associaton for the Study of the Liver - Single Topic Conference 2017
  • Int'l Joint Research
• [Presentation] Hepatic aryl hydrocarbon receptor-mediated lipid droplets production regulates the assembly efficacy of hepatitis C virus. (2017)
  • Author(s): Hirofumi Ohashi, Syo Nakajima, Sulyi Kim, Ryosuke Suzuki, Hideki Aizaki, Masayoshi Fukasawa, Shinji Kamisuki, Fumio Sugawara, Naoko Ohtani, Takaji Wakita, Koichi Watashi
  • Organizer: 24th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Induction of neutralization E2 antibody by immunization of recombinant E2 proteins (2017)
  • Author(s): Noriyuki Watanabe, Tomoko Date, Hussein Aly, Hideki Aizaki, Takaji Wakita
  • Organizer: AASLD The Liver Meeting
  • Int'l Joint Research