| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Addition of a dopamine synthesis inhibitor or a monoamine transporter (VMAT2) inhibitor to the medium improves the differentiation efficiency and maturity of human iPS cell-derived β-like cells. The main cause of this was suppression of dedifferentiation.
Weak insulin secretion observed during the process of β-like cell differentiation and maturation suppressed an increase in the expression of genes that promote cell maturation. Furthermore, this insulin secretion promoted dedifferentiation. In addition, the proportion of cells having dopamine synthetic ability was different for each pancreatic islet. Dopamine, which is released extracellularly during insulin secretion, may be involved in heterogeneous maintenance of β-cell differentiation levels within the islets.
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