| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Selenoprotein P (SeP) is a diabetes-associated hepatokine. Analysis of the interaction between SeP and its receptors makes it possible to screen new compounds as potential anti-diabetic drugs. In this study, we have analyzed the interaction between SeP and its receptors such as ApoER2 by surface plasmon resonance (SPR) binding analysis.
SPR revealed the unique binding mode; 1) SeP binding to ApoER2 was quit strong, 2) the SeP binding site would not be the ligand binding domains but the other domains such as the beta-propeller domain in ApoER2. The strong binding of SeP to the receptor would have an advantage in uptake of selenium to cells. To investigate more details of the interaction, we are now trying to crystallize SeP/receptor complex. Also, the interaction between SeP and LRP1 that is the receptor in the skeletal muscle will be analyse by SPR.
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