Establishment of quantification method of glucagon-related peptides and elucidation of pathophysiological role of the peptides
| Project/Area Number |
17K09831
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Kobe University |
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Principal Investigator |
Minami Kohtaro 神戸大学, 医学研究科, 客員准教授 (80334176)
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| Co-Investigator(Kenkyū-buntansha) |
横井 伯英 神戸大学, 医学研究科, 医学研究員 (70311610)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ペプチドホルモン / グルカゴン / 質量分析計 / 固相抽出法 / 糖尿病 / 免疫沈降法 |
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| Outline of Final Research Achievements |
Peptide hormones exert various physiological roles and dysfunction of their secretion and action closely relate with the onset and pathophysiology of various diseases. Therefore, precise quantification of peptide hormones is essential for the disease diagnosis, pathophysiological evaluation, and assessment of therapeutic effect. In this study, we established an extraction method for glucagon and glucagon-related peptides from plasma samples, and also developed a quantification method by using mass spectrometry. We further confirm the usefulness of the method by using blood samples from animal models of obesity and diabetes and humans.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究において確立されたペプチドホルモンの測定法は、従来の免疫学的な測定法では回避することが困難である抗体の交差性の問題が解消できること、複雑なプロセッシングを受けるホルモンでも高精度な測定が可能であることなど、これまでの測定法と比較して優位性かつ独創性が高いものであり、微量な生体ペプチドを定量性・特異性を担保し迅速・効率的に測定する普遍的な方法の確立に直結する。また、本測定法により新規グルカゴン関連ペプチドの病態生理学的意義が解明されれば、糖尿病などの糖代謝疾患の病態解明に大きく寄与し、その新規診断法確立の基盤となることが期待される。
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] GCN2 regulates pancreatic β-cell mass by sensing intracellular amino acid levels.2020
Author(s)
Kanno A, Asahara SI, Furubayashi A, Masuda K, Yoshitomi R, Suzuki E, Takai T, Kimura-Koyanagi M, Matsuda T, Bartolome A, Hirota Y, Yokoi N, Inaba Y, Inoue H, Matsumoto M, Inoue K, Abe T, Wei FY, Tomizawa K, Ogawa W, Seino S, Kasuga M, Kido Y.
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Journal Title
JCI Insight.
Volume: 5
Issue: 9
Pages: 128820-128820
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Tumor-like features of gene expression and metabolic profiles in enlarged pancreatic islets are associated with impaired incretin-induced insulin secretion in obese diabetes: a study of ZFDM rat.2020
Author(s)
Hayami T, Yokoi N, Yamaguchi T, Honda K, Murao N, Takahashi H, Wang S, Seino Y, Kamiya H, Yabe D, Sweet IR, Mizoguchi A, Nakamura J, Seino S
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Journal Title
J Diabetes Investig
Volume: -
Issue: 6
Pages: 1434-1447
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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