• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Investigation of the role of microRNAs in the formation of liver fibrosis

Research Project

Project/Area Number 17K09860
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Metabolomics
Research InstitutionKyoto University

Principal Investigator

Takahiro Horie  京都大学, 医学研究科, 助教 (20565577)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords非アルコール性脂肪肝炎 / 非コードRNA / マイクロRNA / コレステロール / 脂肪酸 / NASH / SREBP / microRNA / 治療応用 / 内科 / 脂質 / トランスレーショナルリサーチ
Outline of Final Research Achievements

The number of patients with non-alcoholic steatohepatitis is glowing with the westernization of life in our country. Although progression from non-alcoholic steatohepatitis to the development of liver cirrhosis and liver cancer is a concerning issue, the cause has not been clarified and the treatment has not been established. In this study, we investigated the role of microRNAs, a small non-coding RNA that is not translated into proteins of about, in the formation of non-alcoholic steatohepatitis. It became clear that microRNAs could have important roles in the development of non-alcoholic steatohepatitis.

Academic Significance and Societal Importance of the Research Achievements

非アルコール性脂肪肝炎は脂肪肝の患者の1割程度に見られる、肝臓の炎症と線維化を主体とする肝炎である。この病態が進行すると肝硬変、肝がんなどへつながることが示されているが、その成因が明らかでなく根本治療法はない。近年、蛋白にならない遺伝子として非コードRNAに注目が集まっている。本研究から非コードRNAの一つであるマイクロRNAがその成因に大きな役割を果たしていることが明らかとなった。マイクロRNAの発現や機能を制御することが新たな治療法の開発につながると考えられる。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Products Report
  • Research Products

    (35 results)

All 2021 2020 2019 2018 2017 2016 Other

All Journal Article (15 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 15 results,  Open Access: 14 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Int'l Joint Research: 6 results,  Invited: 4 results) Remarks (3 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 1 results)

  • [Journal Article] ABLE-MET Investigators. Effects of Metformin on Left Ventricular Size and Function in Hypertensive Patients with Type 2 Diabetes Mellitus: Results of a Randomized, Controlled, Multicenter, Phase IV Trial.2020

    • Author(s)
      Ono K, Wada H, Satoh-Asahara N, Inoue H, Uehara K, Funada J, Ogo A, Horie T, Fujita M, Shimatsu A, Hasegawa K.
    • Journal Title

      Am J Cardiovasc Drugs.

      Volume: 20 Issue: 3 Pages: 283-293

    • DOI

      10.1007/s40256-019-00381-1

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MiR-33a is a therapeutic target in SPG4-related hereditary spastic paraplegia human neurons2019

    • Author(s)
      Nakazeki F、Tsuge I、Horie T、Imamura K、Tsukita K、Hotta A、Baba O、Kuwabara Y、Nishino T、Nakao T、Nishiga M、Nishi H、Nakashima Y、Ide Y、Koyama S、Kimura M、Tsuji S、Naitoh M、Suzuki S、Izumi Y、Kawarai T、Kaji R、Kimura T、Inoue H、Ono K
    • Journal Title

      Clinical Science

      Volume: 133 Issue: 4 Pages: 583-595

    • DOI

      10.1042/cs20180980

    • NAID

      120006937614

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The identification of differential roles of microRNA-33a and -33b during atherosclerosis progression with genetically modified mice.2019

    • Author(s)
      Koyama S, Horie T, Nishino T, Baba O, Sowa N, Miyasaka Y, Kuwabara Y, Nakao T, Nishiga M, Nishi H, Nakashima Y, Nakazeki F, Ide Y, Kimura M, Tsuji S, Rodriguez RR, Xu S, Yamasaki T, Otani C, Watanabe T, Nakamura T, Hasegawa K, Kimura T, Ono K.
    • Journal Title

      J Am Heart Assoc.

      Volume: 8(13) Issue: 13

    • DOI

      10.1161/jaha.119.012609

    • NAID

      120006818702

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cardioprotective Effects of VCP?Modulator KUS121 in Murine and Porcine Models of Myocardial Infarction2019

    • Author(s)
      Ide Yuya, at al.
    • Journal Title

      JACC: Basic to Translational Science

      Volume: 4 Issue: 6 Pages: 701-714

    • DOI

      10.1016/j.jacbts.2019.06.001

    • NAID

      120006734885

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hepatokine α1-Microglobulin Signaling Exacerbates Inflammation and Disturbs Fibrotic Repair in Mouse Myocardial Infarction2018

    • Author(s)
      Hakuno Daihiko、Kimura Masahiro、Ito Shinji、Satoh Junko、Nakashima Yasuhiro、Horie Takahiro、Kuwabara Yasuhide、Nishiga Masataka、Ide Yuya、Baba Osamu、Nishi Hitoo、Nakao Tetsushi、Nishino Tomohiro、Nakazeki Fumiko、Koyama Satoshi、Hanada Ritsuko、Randolph Ruiz R.、Endo Jin、Kimura Takeshi、Ono Koh
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 1-17

    • DOI

      10.1038/s41598-018-35194-w

    • NAID

      120006645320

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] SREBF1/MicroRNA-33b Axis Exhibits Potent Effect on Unstable Atherosclerotic Plaque Formation In Vivo2018

    • Author(s)
      Nishino T、Horie T、Baba O、Sowa N、Hanada R、Kuwabara Y、Nakao T、Nishiga M、Nishi H、Nakashima Y、Nakazeki F、Ide Y、Koyama S、Kimura M、Nagata M、Yoshida K、Takagi Y、Nakamura T、Hasegawa K、Miyamoto S、Kimura T、Ono K.
    • Journal Title

      Arteriosclerosis, Thrombosis, and Vascular Biology

      Volume: 38 Issue: 10 Pages: 2460-2473

    • DOI

      10.1161/atvbaha.118.311409

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Circulating markers of collagen types I, III, and IV in patients with dilated cardiomyopathy: relationships with myocardial collagen expression2018

    • Author(s)
      Nagao Kazuya、Inada Tsukasa、Tamura Akinori、Kajitani Kenji、Shimamura Kiyotaka、Yukawa Hiroshi、Aida Kenji、Sowa Naoya、Nishiga Masataka、Horie Takahiro、Makita Toshinori、Ono Koh、Tanaka Masaru
    • Journal Title

      ESC Heart Failure

      Volume: 5 Issue: 6 Pages: 1044-1051

    • DOI

      10.1002/ehf2.12360

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Loss of periostin ameliorates adipose tissue inflammation and fibrosis in vivo2018

    • Author(s)
      Nakazeki Fumiko、Nishiga Masataka、Horie Takahiro、Nishi Hitoo、Nakashima Yasuhiro、Baba Osamu、Kuwabara Yasuhide、Nishino Tomohiro、Nakao Tetsushi、Ide Yuya、Koyama Satoshi、Kimura Masahiro、Tsuji Shuhei、Sowa Naoya、Yoshida Shigeo、Conway Simon J.、Yanagita Motoko、Kimura Takeshi、Ono Koh
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 8553-8553

    • DOI

      10.1038/s41598-018-27009-9

    • NAID

      120006510274

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] MicroRNA-33 regulates the population of peripheral inflammatory Ly6Chigh monocytes through dual pathways.2018

    • Author(s)
      Baba O, Horie T, Hakuno D, Nakashima Y, Nishi H, Kuwabara Y, Nakao T, Nishiga M, Nishino T, Ide Y, Nakazeki F, Koyama S, Kimura M, Hanada R, Kawahara M, Kimura T, and Ono K.
    • Journal Title

      Mol Cell Biol.

      Volume: 38 Issue: 14 Pages: 1-17

    • DOI

      10.1128/mcb.00604-17

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Long Non-Coding RNAs as Key Regulators of Cardiovascular Diseases2018

    • Author(s)
      Ono K, Kuwabara Y, Horie T, Kimura T.
    • Journal Title

      Circulation Journal

      Volume: 82 Issue: 5 Pages: 1231-1236

    • DOI

      10.1253/circj.CJ-18-0169

    • NAID

      130006725985

    • ISSN
      1346-9843, 1347-4820
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prevention of neointimal formation using miRNA-126-containing nanoparticle-conjugated stents in a rabbit model.2017

    • Author(s)
      Izuhara M, Kuwabara Y, Saito N, Yamamoto E, Hakuno D, Nakashima Y, Horie T, Baba O, Nishiga M, Nakao T, Nishino T, Nakazeki F, Ide Y, Kimura M, Kimura T, Ono K.
    • Journal Title

      PLoS One

      Volume: 12(3) Issue: 3 Pages: e0172798-e0172798

    • DOI

      10.1371/journal.pone.0172798

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation.2017

    • Author(s)
      Satoshi Koyama, Takashi Kuragaichi, Yukihito Sato, Yasuhide Kuwabara, Shunsuke Usami, Takahiro Horie, Osamu Baba, Daihiko Hakuno, Yasuhiro Nakashima, Tomohiro Nishino, Masataka Nishiga, Tetsushi Nakao, Hidenori Arai, Takeshi Kimura, Koh Ono
    • Journal Title

      ESC Heart Failure

      Volume: 4 Issue: 2 Pages: 112-121

    • DOI

      10.1002/ehf2.12123

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic Ablation of MicroRNA-33 Attenuates Inflammation and Abdominal Aortic Aneurysm Formation via Several Anti-Inflammatory Pathways.2017

    • Author(s)
      Nakao T, Horie T, Baba O, Nishiga M, Nishino T, Izuhara M, Kuwabara Y, Nishi H, Usami S, Nakazeki F, Ide Y, Koyama S, Kimura M, Sowa N, Ohno S, Aoki H, Hasegawa K, Sakamoto K, Minatoya K, Kimura T, Ono K.
    • Journal Title

      Arterioscler Thromb Vasc Biol.

      Volume: 37 Issue: 11 Pages: 2161-2170

    • DOI

      10.1161/atvbaha.117.309768

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a Patient-Derived Induced Pluripotent Stem Cell Model for the Investigation of <i>SCN5A</i>-D1275N-Related Cardiac Sodium Channelopathy2017

    • Author(s)
      Hayano M, Makiyama T
    • Journal Title

      Circulation Journal

      Volume: 81 Issue: 12 Pages: 1783-1791

    • DOI

      10.1253/circj.CJ-17-0064

    • NAID

      130006219109

    • ISSN
      1346-9843, 1347-4820
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] MicroRNA-33 Controls Adaptive Fibrotic Response in the Remodeling Heart by Preserving Lipid Raft Cholesterol.2016

    • Author(s)
      Nishiga M, Horie T, Kuwabara Y, Nagao K, Baba O, Nakao T, Nishino T, Hakuno D, Nakashima Y, Nishi H, Nakazeki F, Ide Y, Koyama S, Kimura M, Hanada R, Nakamura T, Inada T, Hasegawa K, Conway SJ, Kita T, Kimura T, Ono K.
    • Journal Title

      Circ Res.

      Volume: - Issue: 5 Pages: 835-847

    • DOI

      10.1161/circresaha.116.309528

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] MiR-33a は、SPG4 遺伝性痙性対麻痺における新規治療標的である2019

    • Author(s)
      堀江貴裕、中関典子、津下到、今村恵子、木村剛、井上治久、尾野亘
    • Organizer
      第56回日本臨床分子医学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] MiR-33a/b are a novel therapeutical target for vascular disease.2019

    • Author(s)
      Takahiro Horie, Takeshi Kimura, Koh Ono
    • Organizer
      第51回日本動脈硬化学会総会・学術集会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] The identification of differential roles of microRNA-33a and -33b during atherosclerosis progression with genetically modified mice.2019

    • Author(s)
      Takahiro Horie, Satoshi Koyama, Takeshi Kimura, Koh Ono
    • Organizer
      2019 XXIII ISHR WORLD CONGRESS
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of Differential Roles of Microrna-33a and -33b During Atherosclerosis Progression with Genetically Modified Mice.2019

    • Author(s)
      Takahiro Horie, Satoshi Koyama, Yui Miyasaka, Takeshi Kimura, Koh Ono
    • Organizer
      Basic Cardiovascular Sciences Scientific Sessions 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of Differential Roles of Microrna-33a and -33b During Atherosclerosis Progression with Genetically Modified Mice2019

    • Author(s)
      Takahiro Horie, Satoshi Koyama, Yui Miyasaka, Takeshi Kimura, Koh Ono
    • Organizer
      ESC Congress 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] SREBF1/MicroRNA-33b axis exhibits potent effect on unstable atherosclerotic plaque formation in vivo2018

    • Author(s)
      Takahiro Horie、Tomohiro Nishino、Tetsushi Nakao、Fumiko Nakazeki、Yuya Ide、Satoshi Koyama、Masahiro Kimura、Koji Hasegawa、Takeshi Kimura、Koh Ono
    • Organizer
      Asian Pacific Society of Cardiology (APSC) Congress 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Loss of periostin ameliorates adipose tissue inflammation and fibrosis in vivo2018

    • Author(s)
      Takahiro Horie, Fumiko Nakazeki, Masataka Nishiga, Takahiro Horie, Hitoo Nishi, Yasuhiro Nakashima, Osamu Baba, Yasuhide Kuwabara, Tomohiro Nishino, Tetsushi Nakao, Yuya Ide, Satoshi Koyama, Masahiro Kimura, Shuhei Tsuji, Naoya Sowa, Takeshi Kimura, and Koh Ono
    • Organizer
      第50回日本動脈硬化学会学術集会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Loss of periostin ameliorates adipose tissue inflammation and fibrosis in vivo2018

    • Author(s)
      Takahiro Horie、Fumiko Nakazeki、Takeshi Kimura、Koh Ono
    • Organizer
      The 2nd JCS Council Forum on Basic CaridoVascular Research
    • Related Report
      2018 Research-status Report
  • [Presentation] SREBF1/MicroRNA-33b axis exhibits potent effect on unstable atherosclerotic plaque formation in vivo2018

    • Author(s)
      Takahiro Horie、Tomohito Nishino、Takeshi Kimura、Koh Ono
    • Organizer
      The 2nd JCS Council Forum on Basic CaridoVascular Research
    • Related Report
      2018 Research-status Report
  • [Presentation] MicroRNA in Atherosclerosis (JCS-KSC Joint Symposium (Basic Research)2017

    • Author(s)
      Takahiro Horie
    • Organizer
      The 61st Annual Scientific Meeting of The Korean Society of Cardiology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 動脈硬化性疾患に対する核酸医薬の可能性(シンポジウム 13 : 脂質異常症治療薬の新展開)2017

    • Author(s)
      堀江貴裕、木村剛、尾野亘
    • Organizer
      第65回日本心臓病学会学術集会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Serum HDL Cholesterol decreases in microRNA-33b knock-in mice for an intron of sterol regulatory element-binding factor 1 (Srebf1).2017

    • Author(s)
      Takahiro Horie
    • Organizer
      THE 43rd NAITO CONFERENCE Noncoding RNA :Biology, Chemistry, & Diseases
    • Related Report
      2017 Research-status Report
  • [Presentation] MicroRNA-33b promotes atherosclerotic plaque formation in Apoe-/- mice.2017

    • Author(s)
      Nishino T, Horie T, Kuwabara Y, Nakao T, Nishiga M, Ide Y, Nakazeki F, Koyama S, Kimura M, Kimura T, Ono K
    • Organizer
      American Heart Association Scientific Sessions 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Remarks]

    • URL

      http://kyoto-u-cardio.jp/kisokenkyu/metabolic/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 京都大学医学部附属病院 循環器内科 分子循環器グループ

    • URL

      http://kyoto-u-cardio.jp/kisokenkyu/metabolic/

    • Related Report
      2018 Research-status Report
  • [Remarks] 京都大学医学部附属病院 循環器内科 心血管疾患分子機構解明グループ

    • URL

      http://kyoto-u-cardio.jp/kisokenkyu/metabolic/

    • Related Report
      2017 Research-status Report
  • [Patent(Industrial Property Rights)] NASHの予防及び/又は治療剤2021

    • Inventor(s)
      尾野亘, 堀江貴裕, 西野共達, 宮川紗和, 小比賀聡, 笠原勇矢
    • Industrial Property Rights Holder
      国立大学法人京都大学 国立大学法人大阪大学 国立研究開発法人医薬基盤・健康・栄養研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-097796
    • Filing Date
      2021
    • Related Report
      Products Report
    • Overseas
  • [Patent(Industrial Property Rights)] microRNA(miR)-33aおよびmiR-33bを個別に制御できる合成核酸医薬の開発と大動脈瘤に対する効果2019

    • Inventor(s)
      尾野 亘、堀江貴裕、西野共達、山崎智弘
    • Industrial Property Rights Holder
      尾野 亘、堀江貴裕、西野共達、山崎智弘
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 心筋細胞の保護用の医薬組成物2019

    • Inventor(s)
      垣塚彰、尾野 亘、堀江貴裕、井手裕也、斉藤成達、木村剛
    • Industrial Property Rights Holder
      垣塚彰、尾野 亘、堀江貴裕、井手裕也、斉藤成達、木村剛
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] miR-33b ノックインマウスによる非アルコール性脂肪肝炎モデル2017

    • Inventor(s)
      尾野 亘、堀江貴裕、西野共達
    • Industrial Property Rights Holder
      尾野 亘、堀江貴裕、西野共達
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-059580
    • Filing Date
      2017
    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2023-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi