Developing the method for induction of hypothalamic and pituitary unit
| Project/Area Number |
17K09878
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Endocrinology
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
Suga Hidetaka 名古屋大学, 医学系研究科, 准教授 (20569818)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
有馬 寛 名古屋大学, 医学系研究科, 教授 (50422770)
|
|---|
| Project Period (FY) |
2017-04-01 – 2020-03-31
|
| Project Status |
Completed (Fiscal Year 2019)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
|
|---|
| Keywords | 視床下部 / 下垂体 / ヒト多能性幹細胞 / オルガノイド / 低血糖 / 再生医学 / 発生学 / ヒトiPS細胞 / 視床下部-下垂体ユニット / CRH-ACTH / ヒト分化モデル / 視床下部ニューロン / CRH / 内分泌学 |
|---|
| Outline of Final Research Achievements |
The pituitary gland plays an important role in maintaining homeostasis in the living body. It functions under the control of the hypothalamus. In the current hormone replacement therapy for clinical hypopituitarism, it may not be possible to reproduce the blood hormone changes that should be finely tuned, because the function of the hypothalamus cannot be compensated. To solve this problem in regenerative medicine using human ES / iPS cells, we have developed a technique for simultaneous generation of the pituitary gland and the hypothalamus, which is the upper control organ to pituitary. In the induced cell-aggregate, hypothalamic tissue and pituitary tissue coexist and linked functionally.
|
| Academic Significance and Societal Importance of the Research Achievements |
視床下部と下垂体が機能的に連携する仕組みを試験管内で初めて再現可能とし、低血糖など周囲の環境に反応する機能的な組織となっていることが判明した。下垂体機能低下症の再生医療においてより精密なホルモン分泌を再現できる新しい技術になり得る。加えて、下垂体と視床下部の両方を有した組織を試験管内で再現できているため、下垂体と視床下部の関連を解明する上でのモデルとしても期待でき、下垂体疾患の発症メカニズムの解明にも役立つと考えられる。
|
Report
(4 results)
Research Products
(52 results)
-
[Journal Article] Hypothalamic Contribution to Pituitary Functions Is Recapitulated In Vitro Using 3D-Cultured Human iPS Cells.2020
Author(s)
Kasai T, Suga H, Sakakibara M, Ozone C, Matsumoto R, Kano M, Mitsumoto K, Ogawa K, Kodani Y, Nagasaki H, Inoshita N, Sugiyama M, Onoue T, Tsunekawa T, Ito Y, Takagi H, Hagiwara D, Iwama S, Goto M, Banno R, Takahashi J, Arima H
-
Journal Title
Cell Reports
Volume: 30
Issue: 1
Pages: 18-24
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Journal Article] Tanycyte-like cells derived from mouse embryonic stem culture show hypothalamic neural stem/progenitor cell functions.2019
Author(s)
Kano M, Suga H, Ishihara T, Sakakibara M, Soen M, Yamada T, Ozaki H, Mitsumoto K, Kasai T, Sugiyama M, Onoue T, Tsunekawa T, Takagi H, Hagiwara D, Ito Y, Iwama S, Goto M, Banno R, and Arima H.
-
Journal Title
Endoclinology
Volume: 160
Issue: 7
Pages: 1701-1718
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Improved methods for the differentiation of hypothalamic vasopressin neurons using mouse induced pluripotent stem cells.2019
Author(s)
Mitsumoto K, Suga H, Sakakibara M, Soen M, Yamada T,Ozaki H, Nagai T, Kano M, Kasai T, Ozone C, Ogawa K, Sugiyama M, Onoue T, Tsunekawa T, Takagi H, Hagiwara D, Ito Y, Iwama S, Goto M, Banno R, Arima H.
-
Journal Title
Stem Cell Research
Volume: 40:101572
Pages: 101572-101572
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
[Journal Article] Chemical chaperone 4-phenylbutylate reduces mutant protein accumulation in the endoplasmic reticulum of arginine vasopressin neurons in a mouse model for familial neurohypophysial diabetes insipidus.2018
Author(s)
Tochiya M, Hagiwara D, Azuma Y, Miyata T, Morishita Y, Suga H, Onoue T, Tsunekawa T, Takagi H, Ito Y, Iwama S, Goto M, Banno R, Arima H.
-
Journal Title
Neurosci Lett.
Volume: 24
Pages: 50-55
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors2018
Author(s)
Koichiro Ogawa, Hidetaka Suga, Chikafumi Ozone, Mayuko Kano, Kazuki Mitsumoto, Takatoshi Kasai, Yu Kodani, Hiroshi Nagasaki, Naoki Yamamoto, Daisuke Hagiwara, Motomitsu Goto, Ryoichi Banno, Yoshihisa Sugimura, and Hiroshi Arima
-
Journal Title
Scientific Reports
Volume: 8
Issue: 1
Pages: 3615-3615
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
