| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
It has been previously reported that KCNQ1 and EIF2AK4 were identified as T2DM susceptibility genes. We have clarified that the reduction of non-coding RNA Kcnq1ot1 expression induced pancratic beta cell failure in Kcnq1 mutant mice. Now, we analyzed the mechanism how T2DM susceptibility gene induces diabetes using human iPS cells derived pancreatic endocrine cell. As a result, we clarified that two iPS cells were differentiated into pancreatic endocrine cells. One cell has a risk allele of T2DM susceptibility gene, and the other without risl allele. The hiPS cell with a risk allele showed a reduced expression of Kcnq1ot1 compared to pancreatic endocrine cell without a risk allele.
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