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Identification of tissue-resident macrophage progenitors and the development of reprogramming technology to induce the cells

Research Project

Project/Area Number 17K09950
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Hematology
Research InstitutionMie University

Principal Investigator

YAMANE TOSHIYUKI  三重大学, 医学系研究科, 准教授 (30452220)

Co-Investigator(Kenkyū-buntansha) 山崎 英俊  三重大学, 医学系研究科, 教授 (00283987)
Project Period (FY) 2017-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsマクロファージ / 造血発生 / 免疫発生 / 卵黄嚢 / 細胞分化 / リプログラミング / マウス / 組織滞在型マクロファージ / 破骨細胞 / 転写因子 / サイトカイン / 幹細胞 / 造血 / 発生・分化 / 再生医学
Outline of Final Research Achievements

Tissue-resident macrophages are derived from the yolk sac tissue present during embryonic period. It is known that tissue-resident macrophages self-renew independently from hematopoietic stem cells. However, it was not precisely known which type of hematopoietic cells in the yolk sac give rise to macrophage lineage cells. In this study, our analysis showed that the earliest hematopoietic cells that generate primitive erythroid cells and lympho-myeloid lineage cells are the initial source of macrophage lineage cells in the yolk sac. A technology that allows the development of macrophages from mouse fibroblasts was also developed based on the knowledges obtained from the analysis of yolk sac hematopoiesis.

Academic Significance and Societal Importance of the Research Achievements

成体に存在するマクロファージ系譜の多起源性を明らかにするとともに、リンパ球系譜や成体型の赤血球系譜、好中球などの他のミエロイド系譜に先立ち、マクロファージ系譜が発生し、胎仔型赤血球とともに、早期にマクロファージ系譜が全身に播種することを示し、マクロファージ系譜の正常な個体発生への寄与が示唆された。また転写因子によるリプログラミングによって、線維芽細胞からマクロファージを作製できることから、ヒトへと応用できれば、テーラーメイドのマクロファージによるドラッグスクリーニングなど、諸々の疾患の治療法の改善へ寄与する可能性もある。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (17 results)

All 2022 2021 2020 2019 2018 2017 Other

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (9 results) Remarks (3 results)

  • [Journal Article] Multiple cell populations generate macrophage progenitors in the early yolk sac2022

    • Author(s)
      Ito C, Hikosaka-Kuniishi M, Yamazaki H, Yamane T
    • Journal Title

      Cellular and Molecular Life Sciences

      Volume: 79 Issue: 3 Pages: 159-159

    • DOI

      10.1007/s00018-022-04203-7

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Isolation of CD35+ follicular dendritic cells and its role in the differentiation from B cells to IgA+GL7+ cells2022

    • Author(s)
      Hikosaka-Kuniishi M, Yamane T, Isono K, Tetteh DN, Yamazaki H
    • Journal Title

      Immunology Letters

      Volume: 243 Pages: 53-60

    • DOI

      10.1016/j.imlet.2022.02.005

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous Fluorescent Identification of Odontoblasts and Ameloblasts2021

    • Author(s)
      Isono K, Takahashi E, Miyoshi I, Tsuneto M, Hikosaka-Kuniishi M, Yamane T, Yamazaki H
    • Journal Title

      J. Dent. Res.

      Volume: 100 Issue: 5 Pages: 532-541

    • DOI

      10.1177/0022034520974576

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cellular Basis of Embryonic Hematopoiesis and Its Implications in Prenatal Erythropoiesis2020

    • Author(s)
      Yamane T
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 21 Issue: 24 Pages: 9346-9346

    • DOI

      10.3390/ijms21249346

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mouse yolk sac hematopoiesis2018

    • Author(s)
      Toshiyuki Yamane
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 6 Pages: 80-80

    • DOI

      10.3389/fcell.2018.00080

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Follicular dendritic cell-mediated enhancement of the differentiation into IgA+GL7+ cells2021

    • Author(s)
      Hikosaka-Kuniishi M, Yamane T, Yamazaki H
    • Organizer
      第50回 日本免疫学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Yolk sac progenitors for tissue-resident macrophages2019

    • Author(s)
      Toshiyuki Yamane
    • Organizer
      第81回 日本血液学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] Multiple cell populations generate macrophage progenitors in the early yolk sac2019

    • Author(s)
      Toshiyuki Yamane, Mari Hikosaka-Kuniishi, Hidetoshi Yamazaki
    • Organizer
      第48回 日本免疫学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] The role of neural crest-derived cells in thymic regeneration2019

    • Author(s)
      Doris Narki Tetteh, Mari Hikosaka-Kuniishi, Toshiyuki Yamane, Hidetoshi Yamazaki
    • Organizer
      第48回 日本免疫学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] Involvement of follicular dendritic cells in each LNs with IgA production2019

    • Author(s)
      Mari Hikosaka-Kuniishi, Toshiyuki Yamane, Hidetoshi Yamazaki
    • Organizer
      第48回 日本免疫学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] Yolk sac progenitors for tissue-resident macrophages2018

    • Author(s)
      Toshiyuki Yamane, Mari Hikosaka, Hidetoshi Yamazaki
    • Organizer
      第47回 日本免疫学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] Depletion of neural crest-derived cells leads to reduction of plasma noradrenalin and alters T lymphopoiesis2018

    • Author(s)
      Hidetoshi Yamazaki, Mari Hikosaka, Toshiyuki Yamane
    • Organizer
      第47回 日本免疫学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] An attempt to detect follicular dendritic cells in ectopic lymphoid tissues2018

    • Author(s)
      Mari Hikosaka, Toshiyuki Yamane, Hidetoshi Yamazaki
    • Organizer
      第47回 日本免疫学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 卵黄嚢造血の発生過程およびその分子メカニズムの解明2017

    • Author(s)
      山根利之
    • Organizer
      第40回 日本分子生物学会年会 (生命科学系学会合同年次大会)
    • Related Report
      2017 Research-status Report
  • [Remarks] 三重大学大学院医学系研究科幹細胞発生学ホームページ

    • URL

      https://www.medic.mie-u.ac.jp/physiol_regener/

    • Related Report
      2021 Annual Research Report
  • [Remarks] 三重大学大学院医学系研究科幹細胞発生学分野

    • URL

      https://www.medic.mie-u.ac.jp/physiol_regener/

    • Related Report
      2020 Research-status Report
  • [Remarks] 三重大学大学院医学系研究科幹細胞発生学分野ホームページ

    • URL

      http://www.medic.mie-u.ac.jp/physiol_regener/

    • Related Report
      2018 Research-status Report 2017 Research-status Report

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Published: 2017-04-28   Modified: 2023-01-30  

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