| Project/Area Number |
17K10022
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 克也 長崎大学, 医歯薬学総合研究科(保健学科), 教授 (70398147)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 孤発性CJD / QUIC法 / クロイツフェルト・ヤコブ病 / プリオン高感度検出法 |
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| Outline of Final Research Achievements |
Sporadic Creutzfeldt-Jakob disease (sCJD), a majority of human prion diseases, is classified in different subtypes which contribute to the occurrence of distinct clinical-pathological phenotypes. In this study, we tested whether a modification of the real-time quaking-induced conversion (RT-QUIC) assay, a sensitive diagnostic test for prion disease, could distinguish subtypes of sCJD. We prepared various PrP peptides and used them in the QUIC assay to derive those that show a promoting or inhibiting effect. RT-QUICs were performed with brain homogenates from several types of prion disease patients. As a result, by using several PrP peptide, there were slight differences in the RT-QUIC signaling between specimens. Furthermore, the use of multiple peptides further affected the RT-QUIC signal between specimens.
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| Academic Significance and Societal Importance of the Research Achievements |
急速な神経細胞変性をきたす致死性の疾患、プリオン病。その代表的な病気である孤発性クロイツフェルト・ヤコブ病（CJD）はタイプにより予後の進行状況が異なることから、発症早期にタイプを含めた孤発性CJDを正確に診断することが臨床現場から切望されている。ここでプリオンタイプによりRT-QUIC法でのPrP重要配列が異なることが示唆されたことは、予後診断法の確立において大きな意義があると考える。
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