| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In this study, we have developed a technique for an efficient conversion of peripheral blood mononuclear cells into neurons using Sendai virus system. It is faster and more survival efficient than previously reported. This method produced glutamatergic neurons as the main population as the previous methods, but it also included GABAergic and dopaminergic neurons. These results suggested that it will be possible in the future to create selective neural subtypes by adjusting or adding another differentiation factors. In addition, we were successfully able to construct efficient platforms using high-content cell-image analyzers and a single-cell RNA sequencing for validating the character of the cells produced by the culture systems like this study.
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