Relationship between the variation of skin microbiome and atopic dermatitis

• Project/Area Number: 17K10116
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kochi University
• Principal Investigator: FUJIEDA Mikiya 高知大学, 教育研究部医療学系臨床医学部門, 教授 (60209020)
• Co-Investigator(Kenkyū-buntansha): 橋田 裕美子 高知大学, 教育研究部医療学系基礎医学部門, 助教 (00767999) ; 大畑 雅典 高知大学, 教育研究部医療学系基礎医学部門, 教授 (50263976)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: アトピー性皮膚炎 / ウイルス / 微生物 / 感染症 / 細菌 / 炎症性皮膚疾患 / アレルギー / 炎症

Outline of Final Research Achievements

Human skin microbiome has been suggested to have an association with various skin diseases. However, most studies have focused on bacterial communities and little is known about possible association between resident skin viruses and cutaneous disorders such as atopic dermatitis (AD). We investigated the infection levels of skin polyomaviruses including Merkel cell polyomavirus (MCPyV), human polyomavirus 6 (HPyV6) and HPyV7, using skin swabs collected from lesional and nonlesional skins of children with AD, and from healthy control children.
This age-matched case-control study provides the first analyses of the loads of polyomaviruses in association with AD in children. There were no significant differences in viral loads of these polyomaviruses. By contrast, in the skins of patients with AD in adults, the HPyV6 load was significantly higher and the MCPyV load was significantly lower. These results suggest that status of polyomaviruses differs between ADs in children and adults.

Academic Significance and Societal Importance of the Research Achievements

人体のさまざまな部位にはマイクロバイオーム（微生物叢）があり、疾患との関わりが注目されている。微生物叢は皮膚にも存在するが、皮膚マイクロバイオームの変化がどのような疾患に、どのように関与しているのかはいまだ不明な点が多い。これまでの研究では細菌叢に主眼が置かれることが多く、ウイルス叢と皮膚疾患の関係については明らかにされていなかった。
本研究により、成人と小児のアトピー性皮膚炎の間では皮膚常在ウイルスの量的バランスに差異があることが初めて示され、不足している本邦の皮膚マイクロバイオームデータの充足に貢献できたと考えられる。

Research Products

• [Journal Article] Association between nausea and vomiting of pregnancy and postpartum depression: the Japan Environment and Children's Study. (2020)
  • Author(s): Muchanga SMJ, Eitoku M, Mbelambela EP, Ninomiya H, Iiyama T, Komori K, Yasumitsu-Lovell K, Mitsuda N, Tozin RR, Maeda N, Fujieda M, Suganuma N; Japan Environment and Children’s Study Group.
  • Journal Title: J Psychosom Obstet Gynaecol Volume: 印刷中 Issue: 1 Pages: 2-10
  • DOI: 10.1080/0167482x.2020.1734792
  • Peer Reviewed / Open Access
• [Journal Article] Human polyomavirus 6 with the Asian/Japanese genotype in cases of Kimura disease and angiolymphoid hyperplasia with eosinophilia. (2020)
  • Author(s): Yumiko Hashida, Tomonori Higuchi, Kimiko Nakajima, Takako Ujihara, Ichiro Murakami, Mikiya Fujieda, Shigetoshi Sano, Masanori Daibata.
  • Journal Title: Journal of Investigative Dermatology Volume: 印刷中 Issue: 8 Pages: 1650-1653.e4
  • DOI: 10.1016/j.jid.2019.12.027
  • Peer Reviewed / Open Access
• [Journal Article] Severity of Nausea and Vomiting in Singleton and Twin Pregnancies in Relation to Fetal Sex: The Japan Environment and Children’s Study (JECS) (2019)
  • Author(s): Mitsuda N, Eitoku M, Maeda N, Fujieda M, Suganuma N.
  • Journal Title: Journal of Epidemiology Volume: 29 Issue: 9 Pages: 340-346
  • DOI: 10.2188/jea.JE20180059
  • NAID: 130007703029
  • ISSN: 0917-5040, 1349-9092
  • Year and Date: 2019-09-05
  • Peer Reviewed / Open Access
• [Journal Article] Magnetocardiographic recognition of abnormal depolarization and repolarization in patients with coronary artery lesions caused by Kawasaki disease. (2019)
  • Author(s): Tamaki W, Tsuda E, Hashimoto S, Toyomasa T, Fujieda M.
  • Journal Title: Heart Vessels Volume: 34 Issue: 10 Pages: 1571-1579
  • DOI: 10.1007/s00380-019-01409-x
  • Peer Reviewed
• [Journal Article] Myosin heavy chain, a novel allergen for fish allergy in patients with atopic dermatitis. (2019)
  • Author(s): Shibata Y, Serada S, Fujimoto M, Oishi T, Ohko K, Fujieda M, Naka T, Sano S.
  • Journal Title: Br J Dermatol Volume: 181 Issue: 6 Pages: 1322-1324
  • DOI: 10.1111/bjd.18226
  • Peer Reviewed
• [Journal Article] Breastfeeding and risk of febrile seizures in infants: The Japan Environment and Children's Study. (2019)
  • Author(s): Mitsuda N, Hosokawa T, Eitoku M, Fujieda M, Suganuma N; Japan Environment and Children's Study (JECS) Group.
  • Journal Title: Brain Dev Volume: 41 Issue: 10 Pages: 839-847
  • DOI: 10.1016/j.braindev.2019.07.001
  • Peer Reviewed
• [Journal Article] Induction of regional chemokine expression in response to human umbilical cord blood cell infusion in the neonatal mouse ischemia-reperfusion brain injury model (2019)
  • Author(s): Baba Nobuyasu、Wang Feifei、Iizuka Michiro、Shen Yuan、Yamashita Tatsuyuki、Takaishi Kimiko、Tsuru Emi、Matsushima Sachio、Miyamura Mitsuhiko、Fujieda Mikiya、Tsuda Masayuki、Sagara Yusuke、Maeda Nagamasa
  • Journal Title: PLOS ONE Volume: 14 Issue: 9 Pages: e0221111-e0221111
  • DOI: 10.1371/journal.pone.0221111
  • Peer Reviewed / Open Access
• [Journal Article] Common carotid arteritis and polymyalgia with Mycoplasma pneumoniae infection (2019)
  • Author(s): Takahashi Ippei、Ishihara Masayuki、Oishi Taku、Yamamoto Masaki、Narita Mitsuo、Fujieda Mikiya
  • Journal Title: Journal of Infection and Chemotherapy Volume: 25 Issue: 4 Pages: 281-284
  • DOI: 10.1016/j.jiac.2018.09.001
  • Peer Reviewed
• [Journal Article] A consensus statement on health-care transition of patients with childhood-onset chronic kidney diseases: providing adequate medical care in adolescence and young adulthood (2018)
  • Author(s): Kubota Wataru、Honda Masataka、Okada Hirokazu、Hattori Motoshi、Iwano Masayuki、Akioka Yuko、Ashida Akira、Kawasaki Yukihiko、Kiyomoto Hideyasu、Sako Mayumi、Terada Yoshio、Hirano Daishi、Fujieda Mikiya、et al.
  • Journal Title: Clinical and Experimental Nephrology Volume: 22 Issue: 4 Pages: 743-751
  • DOI: 10.1007/s10157-018-1589-8
  • Peer Reviewed
• [Journal Article] Nausea and vomiting during pregnancy associated with lower incidence of preterm births: the Japan Environment and Children’s Study (JECS) (2018)
  • Author(s): Mitsuda Naomi、Eitoku Masamitsu、Yamasaki Keiko、Sakaguchi Masahiko、Yasumitsu-Lovell Kahoko、Maeda Nagamasa、Fujieda Mikiya、Suganuma Narufumi、Japan Environment & Children’s Study (JECS) Group
  • Journal Title: BMC Pregnancy and Childbirth Volume: 18 Issue: 1 Pages: 268-268
  • DOI: 10.1186/s12884-018-1911-1
  • Peer Reviewed / Open Access
• [Journal Article] A nationwide population-based surveillance of invasive Haemophilus influenzae diseases in children after the introduction of the Haemophilus influenzae type b vaccine in Japan (2018)
  • Author(s): Suga Shigeru、Ishiwada Naruhiko、Sasaki Yuko、Akeda Hideki、Nishi Junichiro、Okada Kenji、Fujieda Mikiya、Oda Megumi、Asada Kazutoyo、Nakano Takashi、Saitoh Akihiko、Hosoya Mitsuaki、Togashi Takehiro、Matsuoka Mayumi、Kimura Kouji、Shibayama Keigo
  • Journal Title: Vaccine Volume: 36 Issue: 38 Pages: 5678-5684
  • DOI: 10.1016/j.vaccine.2018.08.029
  • Peer Reviewed / Open Access
• [Journal Article] Generation and characteristics of a novel "double-hit" high grade B-cell lymphoma cell line DH-My6 with MYC/IGH and BCL6/IGH gene arrangements and potential molecular targeted therapies. (2018)
  • Author(s): Hiroaki Kikuchi, Tomonori Higuchi, Yumiko Hashida, Ayuko Taniguchi, Mikio Kamioka, Takahiro Taguchi, Akihito Yokoyama, Ichiro Murakami, Mikiya Fujieda, Masanori Daibata.
  • Journal Title: Oncotarget Volume: 9 Issue: 71 Pages: 33482-33499
  • DOI: 10.18632/oncotarget.26060
  • Peer Reviewed / Open Access
• [Journal Article] IgA polymerization contributes to efficient virus neutralization on human upper respiratory mucosa after intranasal inactivated influenza vaccine administration (2018)
  • Author(s): Terauchi Yoshihiko、Sano Kaori、Ainai Akira、Saito Shinji、Taga Yuki、Ogawa-Goto Kiyoko、Tamura Shin-ichi、Odagiri Takato、Tashiro Masato、Fujieda Mikiya、Suzuki Tadaki、Hasegawa Hideki
  • Journal Title: Human Vaccines & Immunotherapeutics Volume: - Issue: 6 Pages: 1351-1361
  • DOI: 10.1080/21645515.2018.1438791
  • Peer Reviewed
• [Journal Article] Monitoring of Epstein-Barr virus load and killer T cells in patients with juvenile idiopathic arthritis treated with methotrexate or tocilizumab. (2017)
  • Author(s): Mikiya Fujieda, Kazushi Tsuruga, Tetsuya Sato, Hiroaki Kikuchi, Wataru Tamaki, Masayuki Ishihara, Masaki Yamamoto, Taku Oishi, Hiroshi Tanaka, Masanori Daibata.
  • Journal Title: Modern Rheumatology Volume: 27 Issue: 1 Pages: 66-71
  • DOI: 10.1080/14397595.2016.1177247
  • Peer Reviewed
• [Journal Article] The true distribution volume and Bioavailability of mizoribine in children with chronic kidney disease. (2017)
  • Author(s): Nagai T, Uemura O, Kaneda H, Ushijima K, Ohta K, Gotoh Y, Satomura K, Shimizu M, Fujieda M, Morooka M, Yamada M, Wada N, Hashimoto Y.
  • Journal Title: Clin. Exp. Nephrol. Volume: 印刷中 Issue: 5 Pages: 884-888
  • DOI: 10.1007/s10157-016-1353-x
  • Peer Reviewed
• [Journal Article] A Rho-associated coiled-coil containing kinases (ROCK) inhibitor, Y-27632, enhances adhesion, viability and differentiation of human term placenta-derived trophoblasts in vitro (2017)
  • Author(s): Kenichiro Motomura, Naoko Okada, Hideaki Morita, Mariko Hara, Masato Tamari, Keisuke Orimo, Go Matsuda, Ken-Ichi Imadome, Akio Matsuda, Takeshi Nagamatsu, Mikiya Fujieda, Haruhiko Sago, Hirohisa Saito, and Kenji Matsumoto
  • Journal Title: PLOS ONE Volume: in press Issue: 5 Pages: e0177994-e0177994
  • DOI: 10.1371/journal.pone.0177994
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Preconception gynecological risk factors of postpartum depression among Japanese women: The Japan Environment and Children's Study (JECS). (2017)
  • Author(s): Muchanga SM, Yasumitsu-Lovell K, Eitoku M, Mbelambela EP, Ninomiya H, Komori K, Tozin R, Maeda N, Fujieda M, Suganuma N.
  • Journal Title: J Affect Disord. Volume: 217 Pages: 34-41
  • DOI: 10.1016/j.jad.2017.03.049
  • Peer Reviewed / Open Access
• [Journal Article] Incidence and outcomes of uterine rupture among women with prior caesarean section: WHO Multicountry Survey on Maternal and Newborn Health. (2017)
  • Author(s): Motomura K, Ganchimeg T, Nagata C, Ota E, Vogel JP, Betran AP, Torloni MR, Jayaratne K, Jwa SC, Mittal S, Dy Recidoro Z, Matsumoto K, Fujieda M, Nafiou I, Yunis K, Qureshi Z, Souza JP, Mori R.
  • Journal Title: Sci Rep. Volume: 7 Issue: 1 Pages: 44093-44093
  • DOI: 10.1038/srep44093
  • NAID: 120007134943
  • Peer Reviewed / Open Access
• [Presentation] RSウイルス入院患者における中耳炎合併症例の検討 (2019)
  • Author(s): 野村真也，齊藤志穂，寺内芳彦，菊地広朗，石原正行，佐々木剛，久川浩章，長尾明日香，伊藤広明，青井二郎，弘瀬かほり，小森正博，小林泰輔，兵藤政光，藤枝 幹也
  • Organizer: 第51回日本小児感染症学会総会・学術集会
• [Presentation] 川崎病罹患時に発症したウイルス感染症の検討．第55回日本小児循環器学会総会・学術集会 (2019)
  • Author(s): 玉城渉，山本雅樹，藤枝幹也
  • Organizer: 第55回日本小児循環器学会総会・学術集会
• [Presentation] 大きな孤立性の肝膿瘍を呈した猫ひっかき病の1例 (2019)
  • Author(s): 桑名駿介，玉城渉，濱田朋弥，濱本諒，北村祐介，石原正行，久川浩章，山口結花，前田明彦，藤枝幹也
  • Organizer: 第51回日本小児感染症学会総会・学術集会
• [Presentation] 療育上問題のあったアトピー性皮膚炎の1 歳男児2 症例の比較検討. (2018)
  • Author(s): 松岡文弥，萩野紘平，濱本 諒，菊地広朗，玉城 渉，石原正行，山本雅樹，大石 拓，堂野純孝，久川浩章，藤枝幹也.
  • Organizer: 第30回四国小児アレルギー研究会
• [Presentation] 小児腎移植とEBウイルス(EBV)―EBV動態と移植後リンパ増殖症(PTLD)を中心に―． (2018)
  • Author(s): 藤枝幹也
  • Organizer: 第40回日本小児腎不全学会学術集会
  • Invited
• [Presentation] Acetaminophen induced acute tubulointerstitinal nephritis in a 12-year-old boy. (2017)
  • Author(s): Nagao Y, Ishihara M, Fujieda M.
  • Organizer: The 15th Japan-Korea-China Pediatric Nephrology Seminar
  • Int'l Joint Research