| Project/Area Number |
17K10120
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
川崎 幸彦 札幌医科大学, 医学部, 教授 (00305369)
橋本 浩一 福島県立医科大学, 医学部, 准教授 (50322342)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 急性脳症 / 血液脳関門 / 血管内皮障害 / タイトジャンクション / サイトカイン / ウイルス / 血管透過性 / 血管内皮細胞 / 血管内皮電気抵抗 / ウイルス性脳症 / 脳血管内皮細胞 / TNF-α / タイト結合 / 経内皮電気抵抗 |
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| Outline of Final Research Achievements |
The pathogenesis of virus-associated acute encephalopathy (VAE) involves non-inflammatory brain edema caused by disruption of the blood brain barrier (BBB). We aimed to establish an in vitro VAE model using an in vitro BBB model and to evaluate the dynamics of vascular dysfunction caused by tumor necrosis factor (TNF)-α. A co-culture model, consisting of human brain microvascular endothelial cells and pericytes, was treated with serially diluted TNF-α. The VAE model was established by demonstrating that vascular permeability was enhanced in a TNF-α concentration-dependent manner in this evaluation system by transendothelial electrical resistance measurement and solute permeation test.
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| Academic Significance and Societal Importance of the Research Achievements |
脳血管内皮細胞と周皮細胞を用いたBBBモデルを用い、TNF-αを添加することによりin vitroのVAEモデルを作製した。TER測定と溶質透過試験はTNF-αによる血管内皮細胞障害による透過性亢進を評価するのに有用である。この評価方法は、VAEの病態解明だけではなく、血管透過性に焦点をあてた特異的治療法開発に有用であある。
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