Role of miR-21 in chronic lung disease

• Project/Area Number: 17K10186
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Fukushima Medical University
• Principal Investigator: Hayato Go 福島県立医科大学, 医学部, 講師 (30443857)
• Co-Investigator(Kenkyū-buntansha): 桃井 伸緒 福島県立医科大学, 医学部, 教授 (10285033) ; 橋本 浩一 福島県立医科大学, 医学部, 准教授 (50322342)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 新生児慢性肺疾患 / Extracellular vesicles / Exosome / microRNA / miR-21 / エクソソーム / 慢性肺疾患 / miRNA

Outline of Final Research Achievements

Serum EVs were extracted from premature infants at birth and on the 28th day of life (DOL). Using a human miRNA array, we identified 62 miRNAs that were universally expressed in CLD patients and non-CLD patients. Of these miRNAs, serum EV miR-21 was upregulated in CLD patients on DOL28 compared with levels at birth and downregulated in non-CLD patients on DOL28 compared with levels at birth. . We conclude that EV miR-21 may be a biomarker of CLD. In CLD mouse model exposed to hyperoxia, pulmonary function in mice after injection of miR-21 inhibitor was improved. This suggests that miR-21 could be a potential therapeutic target for CLD.

Academic Significance and Societal Importance of the Research Achievements

本研究では、miR-21が新生児慢性肺疾患のバイオマーカーになり得ることを示し、さらに動物実験ではmiR-21を制御することでCLDマウスの呼吸機能が改善していたため、miR-21が新生児慢性肺疾患の治療標的となり得る可能性が示唆された。

Research Products

• [Journal Article] Extracellular vesicle miRNA-21 is a potential biomarker for predicting chronic lung disease in premature infants (2020)
  • Author(s): Hayato Go, Hajime Maeda, Kyohei Miyazaki, Ryo Maeda, Yohei Kume, Fumihiko Namba, Nobuo Momoi, Koichi Hashimoto, Satoru Otsuru, Yukihiko Kawasaki, Mitsuaki Hosoya, and Phyllis A Dennery
  • Journal Title: Am J Physiol Lung Cell Mol Physiol. Volume: 318 Issue: 5 Pages: L845-L851
  • DOI: 10.1152/ajplung.00166.2019
  • Peer Reviewed / Int'l Joint Research
• [Funded Workshop] Pediatric Academic Meeting (2019)