Strategy for the improvement of intestinal mucosal barrier function in low birth weight infants.
Project/Area Number |
17K10192
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Embryonic/Neonatal medicine
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Research Institution | Juntendo University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
東海林 宏道 順天堂大学, 医学(系)研究科(研究院), 准教授 (30365621)
大塚 宜一 順天堂大学, 医学部, 客員准教授 (90338335)
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Project Period (FY) |
2017-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | バリア機能 / 腸管粘膜 / プロバイオティクス / 低出生体重児 / 脂肪酸 / 腸内細菌 / 栄養学 / 感染症 / 微生物 / 免疫学 / 臨床 |
Outline of Final Research Achievements |
We examined the effect of nutritional support and probiotics on the function of intestinal mucosal barrier function in low birth weight infants. The influence of probiotics, including Bifidobacterium longum, Bifidobacterium breve, and Lactobacillus reuteri, on tight-junction function using T84 culture cells. The cells were studied at confluent, pre-confluent, and growing conditions. The mRNA expression of tight-junction proteins, such as TJP1, CLDN1, OCLN, and CLDN3 was evaluated. The mRNA expression of TJP1, CLDN1, and OCLN of pre-confluent and growing conditions was significantly lower than that at confluent condition. After addition of Bifidobacterium CM the expression of TJP1, CLDN1, and OCLN was increased, and especially CLDN3. Moreover, the TJP1 expression was dose-dependently increased.
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Academic Significance and Societal Importance of the Research Achievements |
母乳栄養が低出生体重児の重症感染症やNECの予防に有効であることは臨床上経験されることであり、その機序として母乳成分による抗菌作用や免疫賦活作用、さらには腸内細菌叢に及ぼす影響などが知られている。本研究の結果を踏まえて、低出生体重児特に極低出生体重児に対する理想的な栄養法ならびにプロバイオティクス投与法を実行することで、順調な栄養摂取のみならず、致死的にもなり得る疾病の予防や後の合併症の予防にもつながると考える。
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Report
(5 results)
Research Products
(34 results)
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[Journal Article] Policy Statement of Enteral Nutrition for Preterm and Very Low Birthweight Infants.2020
Author(s)
Mizuno K, Shimizu T, Shinobu Ida, Ito S, Inokuchi M, Ohura T,Okumura A, KawaiM,Kikuchi T, Sakurai M, Sugihara S, Suzuki M,Takitani K,Tanaka D,Mushiake S,Yoshiike N,Kodama H,Kazuo Okada K, Tsutsumi C, Hara M, Hanawa Y,Kawakami K, Inomata H, Oguni T,Bito Y,Uchida K, Akihide Sugiyama A.
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Journal Title
Pediatr Int.
Volume: 62
Issue: 2
Pages: 124-127
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Intrauterine growth restriction affects z-scores of anthropometric parameters during the first 6 years in very low-birth-weight-children born at less than 30 weeks of gestation.2020
Author(s)
Shoji H, Watanabe A, Awaji A, Ikeda N, Hosozawa M, Ohkawa N, Nishizaki N, Hisata K, Kantake M, Obinata K, Shimizu T.
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Journal Title
J Dev Orig Health Dis
Volume: 11
Issue: 1
Pages: 44-48
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Neonatal oral fluid as a transmission route for bifidobacteria to the infant gut immediately after birth.2019
Author(s)
Toda K, Hisata K, Satoh T, Katsumata N, Odamaki T, Mitsuyama E, Katayama T, Kuhara T, Aisaka K, Shimizu T, Xiao JZ.
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Journal Title
Sci Rep
Volume: 9
Issue: 1
Pages: 8692-8692
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Universal recommendations for the management of acute diarrhea in nonmalnourished children.2018
Author(s)
Guarino A, Lo Vecchio A, Dias JA, Berkley JA, Boey C, Bruzzese D, Cohen MB, Cruchet S, Liguoro I, Salazar-Lindo E, Sandhu B, Sherman PM, Shimizu T.
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Journal Title
J Pediatr Gastroenterol Nutr
Volume: 67
Issue: 5
Pages: 586-593
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Rotavirus immunization: Global coverage and local barriers for implementation.2017
Author(s)
Lo Vecchio A, Liguoro I, Dias JA, Berkley JA, Boey C, Cohen MB, Cruchet S, Salazar-Lindo E, Podder S, Sandhu B, Sherman PM, Shimizu T, Guarino A.
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Journal Title
Vaccine
Volume: 35
Issue: 12
Pages: 1637-1644
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Pharmacological inhibition of Notch signaling suppresses food antigen-induced mucosal mast cell hyperplasia.2017
Author(s)
Honjo A, Nakano N, Yamazaki S, Hara M, Uchida K, Kitaura J, Nishiyama C, Yagita H, Ohtsuka Y, Ogawa H, Okumura K, Shimizu T
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Journal Title
J Allergy Clin Immunol.
Volume: 139
Issue: 3
Pages: 987-996
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] The prophylactic antimicrobial therapy criteria for very-low-birth-weight infants promote appropriate antimicrobial therapy.2020
Author(s)
Kasai Y, Hisata K, Toyama Y, Nakano S, Igarashi N, Matsunaga N, Okawa N, Nishizaki N, Komatsu M, Kantake M, Obinata K, Shimizu T.
Organizer
Virtual meeting of The European Society for Paediatric Infectious Diseases.
Related Report
Int'l Joint Research
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