| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
By genome editing, we established epidermis-derived cultured cell lines lacking each of the four major hemidesmosome constituents. Hemidesmosomes were rarely formed in cells lacking plectin or integrin β4 subunit. In type XVII collagen-deficient cells, type II hemidesmosome-like structures were observed, but type I hemidesmosomes were no longer formed. On the other hand, BP230-deficient cells formed type I hemidesmosome-like adhesions. It was suggested that the homotrimer-forming ability of type XVII collagen is important for the assembly of hemidesmosomal constituents into the type I structure.
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