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Novel diagnostic assay for drug hypersensitivity by using basophils

Research Project

Project/Area Number 17K10208
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Dermatology
Research InstitutionMie University

Principal Investigator

Kakeda Masato  三重大学, 医学系研究科, リサーチアソシエイト (00422866)

Co-Investigator(Kenkyū-buntansha) 水谷 仁  三重大学, 医学部, 非常勤講師 (30115737)
山中 恵一  三重大学, 医学系研究科, 教授 (70314135)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords好塩基球 / 薬疹
Outline of Final Research Achievements

In this study, Basophil activation test (BAT) and Drug lymphocyte stimulation test (DLST) were performed in patients with various types of drug hypersensitivity and food allergy. The high sensitivity was detected with both BAT and DLST in severe cutaneous drug reaction such as SJS/TEN and DIHS. Some samples showed positive results for BAT despite negative for DLST. As a result, our results showed BAT is a possible diagnostic assay for drug hypersensitivity and food allergy.

Academic Significance and Societal Importance of the Research Achievements

薬疹は時に生命に関わるほか、重篤な合併症や後遺症をきたすことがあり、正確な対応、そしてその被疑薬剤の同定が臨床上最重要であるが、現在臨床で用いられている各種被疑薬剤同定法にはそれぞれ一長一短がある。我々の研究結果はDLSTと比し少量の採血で可能な好塩基球活性化試験(BAT)が、薬疹の新たな被疑薬剤同定法としての可能性を示した。今後、BATが薬疹被疑薬同定法として健康保険適応となるべく研究を継続したいと考えている。

Report

(3 results)
  • 2019 Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (17 results)

All 2019 2018 2017

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Elevated plasma D-dimer levels in dermatomyositis patients with cutaneous manifestations2019

    • Author(s)
      Habe K, Higashiyama A, Akeda T, Mori R, Kakeda M, Yamanaka K, Mizutani H
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 1410-1410

    • DOI

      10.1038/s41598-018-38108-y

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Infantile bullous pemphigoid successfully treated with i.v. immunoglobulin and cyclosporin2019

    • Author(s)
      Okada K, Kakeda M, Yamamoto S, Yokoyama T, Habe K, Nakato D, Hirayama M, Mizutani H, Yamanaka K
    • Journal Title

      The Journal of Dermatology

      Volume: 46 Issue: 6 Pages: 213-214

    • DOI

      10.1111/1346-8138.14726

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Strong correlation between cancer progression and anti-transcription intermediary factor 1γ antibodies in dermatomyositis patients.2018

    • Author(s)
      Ogawa-Momohara M, Muro Y, Mitsuma T, Katayama M, Yanaba K, Nara M, Kakeda M, Kono M, Akiyama M.
    • Journal Title

      Clin Exp Rheumatol

      Volume: 36 Pages: 990-995

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Plasma Levels of ADAMTS-13, von Willebrand Factor, VWFpp, and Fibrin-Related Markers in Patients With Systemic Sclerosis Having Thrombosis2017

    • Author(s)
      Habe K, Wada H, Higashiyama A, Akeda T, Tsuda K, Mori R, Kakeda M, Matsumoto T, Ohishi K, Yamanaka K, Katayama N, Mizutani H.
    • Journal Title

      Clin Appl Thromb Hemost

      Volume: 23 Issue: 6 Pages: 622-630

    • DOI

      10.1177/1076029617736382

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Successful combination therapy of propranolol and prednisolone for a case with congenital Kasabach-Merritt syndrome.2017

    • Author(s)
      Mizutani K, Umaoka A, Tsuda K, Kakeda M, Habe K, Yamanaka K, Suyama M, Mizutani H.
    • Journal Title

      J Dermatol.

      Volume: 44 Issue: 12 Pages: 1389-1391

    • DOI

      10.1111/1346-8138.13984

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Increased expression of the interleukin-36 cytokines in lesions of hidradenitis suppurativa2017

    • Author(s)
      Thomi R.、Kakeda M.、Yawalkar N.、Schlapbach C.、Hunger R.E.
    • Journal Title

      Journal of the European Academy of Dermatology and Venereology

      Volume: 31 Issue: 12 Pages: 2091-2096

    • DOI

      10.1111/jdv.14389

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Neutrophils are not the dominant interleukin-17 producer in psoriasis.2017

    • Author(s)
      Yamanaka, K., A. Yamagiwa, T. Akeda, M. Kondo, M. Kakeda, K. Habe, S. Imafuku, S. Sano and H. Mizutani
    • Journal Title

      J Dermatol

      Volume: 印刷中 Issue: 7 Pages: 170-171

    • DOI

      10.1111/1346-8138.13807

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Restrictive interleukin-10 induction by an innocuous Parainfluenza virus vector ameliorates nasal allergy.2017

    • Author(s)
      Yamanaka K, Nakanishi T, Isono K, Hasegawa C, Inada Y, Mizutani K, Matsushima Y, Okada K, Mabuchi T, Kondo M, Yamagiwa A, Kakeda M, Habe K, Nosaka T, Gabazza EC, Yamazaki H, Mizutani H, Kawano M.
    • Journal Title

      J Allergy Clin Immunol

      Volume: 139 Issue: 2 Pages: 682-686

    • DOI

      10.1016/j.jaci.2016.05.044

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Is the Anti-NXP-2(ANTI-MJ) antibody a marker antibody for dysphagia in dermatomyositis?-report of three cases2017

    • Author(s)
      Masato Kakeda
    • Journal Title

      EMJ Dermatology

      Volume: 5(1) Pages: 57-58

    • Related Report
      2017 Research-status Report
  • [Presentation] 薬剤性過敏症症候群(DIHS)の7例2019

    • Author(s)
      欠田成人 水谷健人 刑部全晃 波部幸司 山中恵一
    • Organizer
      第1回アレルギー学会東海地方会
    • Related Report
      2018 Research-status Report
  • [Presentation] DIHSの7例2019

    • Author(s)
      欠田成人 水谷健人 刑部全晃 波部幸司 山中恵一
    • Organizer
      第35回三重県アレルギー研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] サラゾスルファピリジンによるDIHSの3例2018

    • Author(s)
      欠田成人 山本晋也 市川彩夏 戸澤貴久 飯田祥平 水谷健人 刑部全晃 後藤啓元 波部幸司 山中恵一
    • Organizer
      第48回日本皮膚免疫アレルギー学会
    • Related Report
      2018 Research-status Report
  • [Presentation] HLA-B*1301保有者に生じたサラゾスルファピリジンによる薬剤性過敏症症候群(DIHS)の2例2018

    • Author(s)
      欠田成人 刑部全晃 戸澤貴久 飯田祥平 水谷健人 後藤啓元 波部幸司 山中恵一
    • Organizer
      第67回日本アレルギー学会学術大会
    • Related Report
      2018 Research-status Report
  • [Presentation] HLA-B*1301-Positive two cases of salazosulfapyridine-induced drug induced hypersensitivity syndrome (DIHS)/ drug reaction with eosinophilia and systemic symptoms (DRESS)2018

    • Author(s)
      Masato Kakeda, Masaaki Gyobu, Takahisa Tozawa, Shouhei Iida, Hiroyuki Goto, Koji Habe, and Keiichi Yamanaka
    • Organizer
      EAACI DHM2018 Drug Hypersensitivity Meeting
    • Related Report
      2018 Research-status Report
  • [Presentation] HLA-B*1301保有者に生じたサラゾスルファピリジンによる薬剤性過敏症症候群(DIHS)の2例2018

    • Author(s)
      欠田成人
    • Organizer
      第67回日本アレルギー学会学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] HLA-B*1301-Positive Two Cases Of Salazosulfapyridine-Induced Drug Induced Hypersensitivity Syndrome (DIHS)/ Drug Reaction With Eosinophilia And Systemic Symptoms (DRESS)2018

    • Author(s)
      Masato Kakeda
    • Organizer
      Drug Hypersensitivity Meeting 2018
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 最近経験した重症薬疹について2018

    • Author(s)
      欠田成人
    • Organizer
      三重県アレルギー研究会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2024-12-25  

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