| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Outline of Final Research Achievements |
(1) Spontaneous dermatitis occurred in keratinocyte-specific ROCK1/2 deficient mice. Histopathologically, epidermal thickening, inflammatory cell infiltration, and an increase in mast cells in the dermis were observed. Elevated levels of IgE, TSLP and IL-6 in serum were also detected. Immunohistochemical staining revealed abnormal distribution or reduction of expression of differentiation-related proteins.
(2) In keratinocyte-specific mDia1/3 deficient mice (K5Cre-mDia1/3KO mice), substance permeability test revealed that the skin permeability barrier was reduced. Levels of mRNA expression of enzymes involved in lipid metabolism, which are important in the formation of the intercellular lipid membrane of the stratum corneum, is not clearly different from those of wild-type mice. Electron microscopic analysis suggested decreased secretion of lamellar bodies in K5Cre-mDia1/3KO mice. Meanwhile, the amount of ceramide in the stratum corneum in K5Cre-mDia1/3KO mice tended to increase.
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