Establishing Novel Embryonic and Neonatal Treatments using Mice with Atopic Dermatitis and Ichthyosis as Models

• Project/Area Number: 17K10234
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: University of Fukui
• Principal Investigator: Chino Takenao 福井大学, 学術研究院医学系部門, 助教 (20521397)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: マウス胎仔・新生児治療 / DMKN / 皮下投与 / マウス胎仔新生児治療 / 魚鱗癬モデルマウス / デルモカイン / マウス胎仔治療 / 移植・再生医療

Outline of Final Research Achievements

Obtaining improvements to the postnatal skin phenotype of DMKN α/β/γ deficient mice which are considered to be animal models of atopic dermatitis and ichthyosis from the embryonic to neonatal periods by administering therapeutics, proteins, cells, etc. are continuing to be researched. Currently, whether or not improvement of skin symptoms could be obtained by subcutaneously administering keratinocyte cells that express DMKN proteins was inspected. The subcutaneous administration to fetuses and newborn mice suggests that the skin phenotype at the administration site was improved. It is thought that this procedure could link to the treatment of newborn human fetuses with skin diseases.

Academic Significance and Societal Importance of the Research Achievements

出生前に全身の皮膚に治療効果のある物質・蛋白・細胞が定着し、出生後の皮膚表現型の改善がみられれば、将来的にアトピー性皮膚炎や魚鱗癬だけでなく、様々な皮膚疾患のヒト胎児・新生児治療につながるものと考えられる。この研究を通して出生後のマウス皮膚の改善が認められれば、様々な難治性皮膚疾患に適用可能な画期的な治療法になりうる点で、大きな意義があると考えられる。将来、拒絶反応が少なく効果が持続しやすいヒト胎児・新生児治療のメリットを生かした皮膚疾患の治療に発展させたい。

Research Products

• [Journal Article] Homeostatic Function of Dermokine in the Skin Barrier and Inflammation. (2020)
  • Author(s): Akira Utsunomiya, Takenao Chino, Natsuko Utsunomiya, Vu Huy Luong, Atsushi Tokuriki, Tatsuro Naganuma, Makoto Arita, Kiyoshi Higashi, Koichi Saito, Noriyuki Suzuki, Ayako Ohara, Manabu Sugai, Koji Sugawara, Daisuke Tsuruta, Noritaka Oyama, Minoru Hasegawa.
  • Journal Title: Joural of Investigative Dermatology. Volume: 140(4) Pages: 838-849
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Dual function of Dermokine in regulating Skin Barrier and Innate immunity. (2019)
  • Author(s): Akira Utsunomiya, Takenao Chino, Natsuko Utsunomiya, Vu Huy Luong, Atsushi Tokuriki, Tatsuro Naganuma, Makoto Arita, Kiyoshi Higashi, Koichi Saito, Manabu Sugai, Koji Sugawara, Daisuke Tsuruta, Noritaka Oyama, Minoru Hasegawa.
  • Organizer: The 44th Annual Meeting of the Japanese Society for Investigative Dermatology.
  • Int'l Joint Research