| Project/Area Number |
17K10504
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
後藤 昌史 東北大学, 医学系研究科, 教授 (50400453)
戸子台 和哲 東北大学, 大学病院, 助教 (50581641)
原 康之 東北大学, 医学系研究科, 非常勤講師 (50636008)
中西 渉 東北大学, 大学病院, 助教 (50636024)
宮澤 恒持 東北大学, 医学系研究科, 非常勤講師 (80757237)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Muse細胞 / 心停止ドナーグラフト / 過小グラフト / 酸素化灌流 / 類洞微小循環 / 移植・再生医療 |
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| Outline of Final Research Achievements |
The treatment for the sinusoidal microcirculatory injury (SMI) induced by excessive shear stress to liver sinusoidal endothelial cells (LSECs) is necessary for the regeneration. Multilineage-differentiating Stress Enduring (Muse) cells selectively home to the injured organ and repair by spontaneously differentiation. We hypothesized that intravenous administration of Muse cells might improve SMI the sinusoid. Then we evaluated the protective effects of Muse cells using an extra-small partial liver transplantation model in rats. The analysis of liver sinusoidal images, indocyanine green test, immunohistochemical stain of LSECs revealed that sinusoidal continuity, volume and surface area were protected significantly by administrated Muse cells. Furthermore, Ki-67 positive ratio of hepatocyte is higher in Muse group. The combination of cell transplantation using Muse cells and organ transplantation of small-for-size grafts is effective as a treatment for end-stage of liver disease.
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| Academic Significance and Societal Importance of the Research Achievements |
肝不全治療における新たな手法、臓器移植と細胞移植のハイブリッド移植という新たな治療の臨床応用へのきっかけを作るこ事ができた。Muse細胞のすぐれた組織修復能力と抗炎症効果が確認でき、今後の再生医療におけるきっかけとなった。過小肝グラフト移植などにおける類洞内皮障害や門脈圧亢進症状（SFSS）の改善というレベルでの効果は、Museの組織修復能力は有効だと確認できた。今後の研究継続が必要である。
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