| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In this study, we investigated the effect of anti-CD20 mAb -mediated B-cell depletion on anti-donor T-cell responses and the inhibitory role of B-cells in a highly sensitized transplant model. The average stimulation index values for CD4+ T-cell responses to donor stimulation were significantly higher in the desensitization group than those in the control group. When co-cultured with various B-cells in the CFSE-MLR assay of the desensitization group, the addition of whole CD19+ B-cells and B1-cells significantly suppressed the anti-donor CD4+ T-cell responses. However, the addition of non B1-cells did not suppress the anti-donor CD4+ T-cell responses. These findings demonstrate that B-cell depletion with anti-CD20 mAb exacerbates anti-donor CD4+ T-cell responses in a highly sensitized transplant model. It might be possible that the sensitized CD5+ CD19+ B1-cells have an ability to inhibit anti-donor CD4+ T-cell responses.
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